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Offlineda-shrooms
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Registered: 01/19/20
Posts: 4
Last seen: 3 years, 1 month
Stalled out? * 1
    #26672379 - 05/15/20 10:18 AM (3 years, 8 months ago)

Hi all. First post here but I've been lurking for a while. I am having an issue that I cannot seem to figure out about my tub. I started to see knots about 2 weeks ago and since then nothing has happened.

This is my first attempt at doing BRF cakes to bulk. I started out with 5 1/2 pint jars that were about 80% colonized. Shredded them up and mixed in with some pasteurized coir/verm with a 1:2 ratio. I mixed it all up and it fully colonized in the tub in just over a week.

Here's a quick time table of this attempt.

3/26 inoculated 8 hp jars with spore syringes. Lost 3 due to contamination.
4/14 shredded and mixed in bulk container
4/24 substrate fully colonized
4/30 some signs of knotting (I think)

I noticed that after it was fully colonized that it started to develop a thick clear goopy substance near the edges. I remove it with some paper towels when I notice it. I would mist two or 3 times a day and leave the lid upside down. I live in a low to no humidity area and it stays around 65 in my home all day. They get ambient light throughout the day.

It started turning a yellowish brown near the corners and looking very dry. I did end up putting on a thin layer of vermiculite and misted a lot. It still looks very dry after lightly brushing away some of it today to examine it closer.

Can I same this and get it fruiting? Or toss it?





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OfflineMugnath
Male

Registered: 05/24/12
Posts: 733
Loc: Oregon
Last seen: 8 months, 16 days
Re: Stalled out? [Re: da-shrooms] * 2
    #26672397 - 05/15/20 10:30 AM (3 years, 8 months ago)

Let them colonize 100% before you take cakes out of jars. Going from a MS syringe to brf cakes to bulk is risky because generally any contaminations within a syringe will become more apparent as you provide contams with new opportunities to colonize fresh substrate.


Edited by Mugnath (05/15/20 10:33 AM)


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Offlineda-shrooms
Stranger
Registered: 01/19/20
Posts: 4
Last seen: 3 years, 1 month
Re: Stalled out? [Re: da-shrooms]
    #26672430 - 05/15/20 10:54 AM (3 years, 8 months ago)

I will be starting to use agar on my next attempt to help reduce any contaminants as well as move away from BRF tek. I'm losing 1 maybe 2 jars before being able to get them into fruiting conditions

Any thoughts if this is at all salvageable?


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OfflineMugnath
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Registered: 05/24/12
Posts: 733
Loc: Oregon
Last seen: 8 months, 16 days
Re: Stalled out? [Re: da-shrooms]
    #26672465 - 05/15/20 11:13 AM (3 years, 8 months ago)

Have you built a SAB yet? I personally would toss it and restart.


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OfflineMushit
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Registered: 02/04/04
Posts: 253
Loc: The Great White North
Last seen: 7 months, 12 days
Re: Stalled out? [Re: Mugnath]
    #26672515 - 05/15/20 11:54 AM (3 years, 8 months ago)

I wouldn't toss it until it totally looks like it is contammed.
Keep fanning and if it looks dry, mist.
Temps are a bit low and this could slow them down.
Patience......


--------------------
Up to my elbows in shit!


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Offlineda-shrooms
Stranger
Registered: 01/19/20
Posts: 4
Last seen: 3 years, 1 month
Re: Stalled out? [Re: da-shrooms]
    #26672521 - 05/15/20 11:56 AM (3 years, 8 months ago)

That is my project this weekend while doing my first agar plates. I've had very limited space and funds to use until recently.


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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,782
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Re: Stalled out? [Re: da-shrooms]
    #26672706 - 05/15/20 01:50 PM (3 years, 8 months ago)

Is that block sitting on verm?


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LAGM2020


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OnlineDispsiple
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Registered: 07/25/18
Posts: 159
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Re: Stalled out? [Re: A.k.a] * 1
    #26672853 - 05/15/20 03:24 PM (3 years, 8 months ago)

Don't move away from pf Tek before you get it right, it will teach you a lot about growing before you get too invested in pressure cookers and all the other fun tools. Is the 1:2 ratio coir:verm or spawn: substrate? It looks like a lot of verm, and as stated above shredding your jars at 80% instead of waiting until 100 was a bad idea as well.


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Offlineda-shrooms
Stranger
Registered: 01/19/20
Posts: 4
Last seen: 3 years, 1 month
Re: Stalled out? [Re: da-shrooms]
    #26672901 - 05/15/20 03:54 PM (3 years, 8 months ago)

I know the temps are on the low side. I tried to warm up the room closer to 70 but it caused issues with the subs getting too dry before I could mist again. I will keep an eye on it for a bit longer and see what it does. I'm not too hopeful for that tub at this point.

No that verm is what I brushed off to get a better view of the sub itself and get pics of it. The sub is fully colonized and it has shrunk quite a bit since I put it in the tub.

The 1:2 ratio is spawn to coir/verm mix. I did 175g coir and 55g verm in that batch.


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OfflineLogicaL ChaosM
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Re: Stalled out? [Re: da-shrooms]
    #26673075 - 05/15/20 04:58 PM (3 years, 8 months ago)

I would suggest using perlite inside of the vermiculite. It looks pretty wet in there. Also, can you raise the temp to 70F with a space heater? That should help.


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