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redhandmat
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Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] 1
#26671921 - 05/15/20 04:24 AM (3 years, 8 months ago) |
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I know I sound crazy, but I'm still not convinced Im growing myc. I have a print (my only print). Tried BRF, didn't go well. Learnt agar, got something that more experienced growers here found to be bacterial plates with myc. Transferred a bunch of time in SAB, growth looked a bit cleaner but still crazy. But growers here explained that MS just looks crazy because spores grow on top of each-other.
I put the agar to WBS jars got a couple of jars colonized, Bod took a look in the thread and told me they dont look healthy so my mileage may vary. But he didnt think it was a bad idea to birth them and clone+print from there. So I did put them to coir in unmodified shoebox following SFF tek.
But before I show pictures, I want to add that I have two more jars that have been finished for a couple weeks now. But Im letting them sit to see if something turns green or other kind of bad. And so far there is only a bit of condensation, which probably means there is a bit of bacterial contamination. And even in the shoebox we can see a couple of yellow-ish spots which Im sure is an indication of bacterial growth. And you know, that sucks, but it wasnt unexpected.
However, I have a worst feeling. I have a feeling that Im growing trich or something that looks like myc but isnt. Smell is fine, no discoloring except for a couple of tinted yellow spots. But the myc doesnt look like other pictures at all.
FYI: Since yesterday I have the lid up and down so it gets FAE. I only misted it lightly twice since I put it to coir, regretted misting though cause it doesnt need to.
Edited by redhandmat (05/16/20 04:12 AM)
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Je77Ce11ar



Registered: 01/09/19
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: redhandmat]
#26671942 - 05/15/20 04:53 AM (3 years, 8 months ago) |
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if you were growing trich itd turn green real quick, how long ago did you spawn this shoebox?
however, that looks hella overlayed, and i doubt you'll get any fruits from that
what did you spawn look like?
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Sockadin



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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: redhandmat]
#26671949 - 05/15/20 04:57 AM (3 years, 8 months ago) |
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I'm gonna guess you get short fat odd looking mushrooms. It looks like I'm healthy Mycelium that is riddle with bacteria. It doesn't look like Trich to me. It's too shiney.
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Ombisha
Transmutant



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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: Je77Ce11ar]
#26671971 - 05/15/20 05:13 AM (3 years, 8 months ago) |
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Quote:
Je77Ce11ar said: if you were growing trich itd turn green real quick, how long ago did you spawn this shoebox?
however, that looks hella overlayed, and i doubt you'll get any fruits from that
what did you spawn look like?
What do you mean by overlayed? I thought that the "overlay" is some obscure condition that almost never happens and that cubes don't even do it.
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redhandmat
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: Sockadin]
#26671975 - 05/15/20 05:16 AM (3 years, 8 months ago) |
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Quote:
Je77Ce11ar said: if you were growing trich itd turn green real quick, how long ago did you spawn this shoebox?
however, that looks hella overlayed, and i doubt you'll get any fruits from that
what did you spawn look like?
If I read the contamination thread correctly it means that myc is all over the casing, and thats why its overlayed? First I thought that it was because my casing was really thin, but thic myc has been weird since the start. And its probably my fault, but I have a feeling that the original print was bad. At least Im happy that trich reacts that fast because Im growing in a small apartment and I wouldn't want a motherload of trich spores in the air. Really sucks that I probably wont get any fruits. I was hoping to at least get one decent shroon to clone. Because this print has been hell.
Here is my spawn, WBS PCd for 2 hours and a halfm nocced with agar in SAB (yeah it doesnt look super nice):

Quote:
Sockadin said: I'm gonna guess you get short fat odd looking mushrooms. It looks like I'm healthy Mycelium that is riddle with bacteria. It doesn't look like Trich to me. It's too shiney.
So second opinion that its not trich, thats a relief. If I do get anything at all, would it be dumb to clone those weird mushrooms? Because my print has been hell to work with. I have tried everything and nothing has worked out to-date.
Edit: forgot to add that it has been 12 days since spawning to bulk.
Edited by redhandmat (05/15/20 05:18 AM)
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Je77Ce11ar



Registered: 01/09/19
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: Ombisha]
#26671979 - 05/15/20 05:19 AM (3 years, 8 months ago) |
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Quote:
Ombisha said:
Quote:
Je77Ce11ar said: if you were growing trich itd turn green real quick, how long ago did you spawn this shoebox?
however, that looks hella overlayed, and i doubt you'll get any fruits from that
what did you spawn look like?
What do you mean by overlayed? I thought that the "overlay" is some obscure condition that almost never happens and that cubes don't even do it.
maybe i just invented my own use of that word for that appearance...as in the "myc" just consumes the casing/top layer and gets all puffy
but yea regardless, you got some contaminated myc there, your spawn jars dont look good at all...you can tell by how fluffy the myc looks
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Roger Clemency
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Registered: 03/23/20
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: Je77Ce11ar]
#26672015 - 05/15/20 06:10 AM (3 years, 8 months ago) |
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I’m guessing you never really got away from the bacteria on your plates. You said they still looked funny but attributed that to spores. The spores only have that kinda funky growth on the germination plate because they’re all coming out of different areas and making a fluffy mess. Once you transfer a piece though it should start growing out normally. And especially for any subsequent transfers.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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redhandmat
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: Roger Clemency]
#26672037 - 05/15/20 06:37 AM (3 years, 8 months ago) |
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Quote:
Roger Clemency said: I’m guessing you never really got away from the bacteria on your plates. You said they still looked funny but attributed that to spores. The spores only have that kinda funky growth on the germination plate because they’re all coming out of different areas and making a fluffy mess. Once you transfer a piece though it should start growing out normally. And especially for any subsequent transfers.
That must be the issue yes. See for instance this plate is a third transfer. Whereby I had started with germination plate, hotpoured it, transferred growth to a new plate, and then transferred from that to this one.

I was happy in the start because it looked much better than the original germination plate. However, this is how it started to look some time later:

I thought at first it acted that way because of the agar recipe I had this time, where I had experimented with a tiny bit of coffee and also the agar didn't go well, had some sediments etc. But now I'm thinking that I probably have taken with me bacterial contamination all the way to the third transfer. And now it looks like this:

So my thinking is this:
I should go back to this last plate, hot pour agar over it, and transfer new growth to a new plate early on. Maybe even try to add some antibiotic if I can get some. Ill ask around (in my country antibiotics are heavily regulated). What do you guys think?
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A.k.a
Stranger



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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: redhandmat]
#26672124 - 05/15/20 07:48 AM (3 years, 8 months ago) |
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What’s in your agar?
That first picture looks like healthy myc on super high nutrient agar to me.
Have you check the other plates to make sure its not the agar itself that’s bacterial?
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LAGM2020     
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Roger Clemency
Smile


Registered: 03/23/20
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: A.k.a]
#26672184 - 05/15/20 08:33 AM (3 years, 8 months ago) |
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That first pic does look pretty good but then things definitely take an odd turn.
What are your options for inoculating? Do you have more spores. I see you have one GT print. I would make plates with a very standard agar recipe. 10g 10g 500ml agar/lme/h20 or whatever nutrient you have and swipe a pattern on it and start over there.
At the same time you could try your hot pour or maybe rub a loop across the top of some myc and try to get a few strands that way but something just seems off about that myc. I believe it’s cube myc but it just looks funny and I really can’t say why.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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redhandmat
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: Roger Clemency]
#26672261 - 05/15/20 09:10 AM (3 years, 8 months ago) |
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Quote:
A.k.a said: What’s in your agar?
That first picture looks like healthy myc on super high nutrient agar to me.
Have you check the other plates to make sure its not the agar itself that’s bacterial?
It definitely was super high nutrient. I had a drop of honey, potato flakes, tiny bit of yeast, agar ofc, some black tea water to make it antibacterial, and a bit of coffee. I know, really crazy, but it did look good to start with. another plate I noced also started to look like that one. my third plate which I only pretended to noc didnt grow anything and so it wasnt my sterile tek.
Quote:
Roger Clemency said: That first pic does look pretty good but then things definitely take an odd turn.
What are your options for inoculating? Do you have more spores. I see you have one GT print. I would make plates with a very standard agar recipe. 10g 10g 500ml agar/lme/h20 or whatever nutrient you have and swipe a pattern on it and start over there.
At the same time you could try your hot pour or maybe rub a loop across the top of some myc and try to get a few strands that way but something just seems off about that myc. I believe it’s cube myc but it just looks funny and I really can’t say why.
My options are the print ofc, but Im so sure its the print genetics or something. I got it from a pretty big supplier so it wouldn't surprise me if their genes just have been diluted to shit. But that seems like a way to offload my responsibility as a grower... I dont have lme but Im gonna order it now with some extra petri to do more pours and the standard agar then. You're right that it looks so weird. Thats why Im hoping to god Ill get a pin or something to clone. I'll also try to check the marketplace for some noob friendly giveaway, I would trust a shroomery print over any commercial one.
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sillysyban
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: redhandmat]
#26673232 - 05/15/20 06:02 PM (3 years, 8 months ago) |
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Im a total Noob but could it be monokaryotic mycelium where only one spore has germinated and it hasn't found a compatible partner? This would create fluffly tomentose type mycelium that wouldn't fruit as far as Im aware. I have a agar dish that I am wondering about too.
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Apples in Mono
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Registered: 09/21/17
Posts: 3,240
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: sillysyban]
#26673324 - 05/15/20 06:40 PM (3 years, 8 months ago) |
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Yeah, looks like it's been bacterial from the start
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redhandmat
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: Apples in Mono]
#26674083 - 05/16/20 03:56 AM (3 years, 8 months ago) |
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It looks even worse today:

Thinking of dumping it and the jars in the woods and see if something grows in a few weeks . I may be getting a new print I can start over from in a couple weeks
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poisoned
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: sillysyban]
#26674119 - 05/16/20 04:22 AM (3 years, 8 months ago) |
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Quote:
sillysyban said: Im a total Noob but could it be monokaryotic mycelium where only one spore has germinated and it hasn't found a compatible partner? This would create fluffly tomentose type mycelium that wouldn't fruit as far as Im aware. I have a agar dish that I am wondering about too.
Chances of that happening are practically zero.
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redhandmat
Dude


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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: Apples in Mono]
#26674190 - 05/16/20 05:32 AM (3 years, 8 months ago) |
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Quote:
Apples in Mono said: Yeah, looks like it's been bacterial from the start
Yeah, it started to look even sadder today. So I put it all in a bag, even the contents of the jar and some of the plates. Im gonna burry it all in the woods later today, and in the meantime gonna clean the entire house thoroughly. Will be waiting for two prints that some amazing shroomery colleagues are sending me and start from scratch.
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A.k.a
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: redhandmat] 1
#26674357 - 05/16/20 08:11 AM (3 years, 8 months ago) |
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Nooooo
It’s not that bad man ride it out til it goes green.
Unless you’ve got clean spawn you need to space for.
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LAGM2020     
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sillysyban
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: poisoned]
#26675333 - 05/16/20 05:07 PM (3 years, 8 months ago) |
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Quote:
sillysyban said: Im a total Noob but could it be monokaryotic mycelium where only one spore has germinated and it hasn't found a compatible partner? This would create fluffly tomentose type mycelium that wouldn't fruit as far as Im aware. I have a agar dish that I am wondering about too.
Chances of that happening are practically zero.
OK cool. Thanks. Been wondering what the chances are for some time now.
Edited by sillysyban (05/16/20 05:08 PM)
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redhandmat
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: A.k.a] 1
#26678196 - 05/18/20 05:04 AM (3 years, 8 months ago) |
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Quote:
A.k.a said: Nooooo
It’s not that bad man ride it out til it goes green.
Unless you’ve got clean spawn you need to space for.
After I read your comment I thought that maybe I was overreacting, so I took a look into the bag and dude, it was the most awful smell ever. Like no way you can live in a house with that smell. Now they are burried somewhere with shade, and I even watered the soil a little. Maybe if we are lucky it will fruit something. If not its ok, a new print is on its way, and this time the germination plate will have some antibiotics in it which hopefully will help a little. Even though, ofc sterile tek is everything.
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Je77Ce11ar



Registered: 01/09/19
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Re: Am I growing trich or myc? First growth [MS agar] [shoebox] [day 12] [Re: redhandmat]
#26679190 - 05/18/20 03:21 PM (3 years, 8 months ago) |
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Antibiotic plates do help, but arent necessary. In 3-4 transfers you should be able to clean up almost anything. Maybe try plates with a little less nutes?
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