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Offlinelucidasans
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Registered: 04/18/20
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Using lids as petri dish * 1
    #26671259 - 05/14/20 07:13 PM (3 years, 8 months ago)

Hello guys, newbie here..

I'm searching an alternative to petri's dish and I found this image



How could it work? There is any way? It seems to be wrapped. Anyway, I know I can use some pp5 plastic pots to substitute, but i'm wondering how could it work.

LS


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Edited by lucidasans (05/27/20 09:48 AM)


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OnlineLogicaL ChaosM
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Re: Using lids as petri dish [Re: lucidasans]
    #26671262 - 05/14/20 07:15 PM (3 years, 8 months ago)

Should work but why not use regular dishes? Are they hard to get or somethang?


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Offlinelucidasans
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Re: Using lids as petri dish [Re: LogicaL Chaos]
    #26671281 - 05/14/20 07:31 PM (3 years, 8 months ago)

Quote:

LogicaL Chaos said:
Should work but why not use regular dishes? Are they hard to get or somethang?




It's difficult to me buying dishes now.

How could it work? Sterilize the lids then pour the agar? Sterilize wrapped? What you think?...

It's about curiosity too.


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Re: Using lids as petri dish [Re: lucidasans]
    #26671292 - 05/14/20 07:41 PM (3 years, 8 months ago)

Fair enough.

Sounds like a good plan. Do u have parafilm to wrap it with?


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Offlinelucidasans
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Re: Using lids as petri dish [Re: LogicaL Chaos] * 1
    #26671318 - 05/14/20 07:50 PM (3 years, 8 months ago)

No, i dont. It seems to be Parafilm®, right?

I was thinking it could be something like PVC. I dont know exactly the name.. Those used to wrap food.

It's really not the same. I think I can forget this technique. Parafilm is too expensive

There is not an alternative... ya? Its just a few things can go to boil, etc..


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Edited by lucidasans (05/14/20 07:52 PM)


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Re: Using lids as petri dish [Re: lucidasans]
    #26671323 - 05/14/20 07:53 PM (3 years, 8 months ago)

Ive heard people using Clingwrap.


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Offlinelucidasans
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Re: Using lids as petri dish [Re: LogicaL Chaos]
    #26671338 - 05/14/20 08:00 PM (3 years, 8 months ago)

Yes! It seems to be the same as "Plastic Wrap" is it? Clingwrap just a brand?...

I was talking about something like this. I have tons of it.

Can it go to PP/oven/steam?


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Edited by lucidasans (05/14/20 08:00 PM)


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Re: Using lids as petri dish [Re: lucidasans]
    #26671344 - 05/14/20 08:02 PM (3 years, 8 months ago)

Not sure what PP is.

Clingwrap is a type of Suran Wrap which is suitable for Petri dishes wrapping after they have cooled (use tin foil as covering in the PC).

Use a SAB box to cover the petri dishes with the Clingwrap.


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Offlinelucidasans
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Re: Using lids as petri dish [Re: LogicaL Chaos]
    #26671356 - 05/14/20 08:09 PM (3 years, 8 months ago)

Sorry, I mean PC!

Just to finish: What is the order? Sterilize wrapped with agar in or pour the agar separately? (All in a SAB)


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Re: Using lids as petri dish [Re: lucidasans]
    #26671358 - 05/14/20 08:11 PM (3 years, 8 months ago)

Wrap agar plates in tin foil, PC them, cool the PC naturally, transfer plates to SAB then use them and/or cover with Clingwrap would be my suggestion.


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Offlinelucidasans
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Re: Using lids as petri dish [Re: LogicaL Chaos]
    #26671362 - 05/14/20 08:13 PM (3 years, 8 months ago)

Thanks VERY MUCH!

I will give it a try.

:smile:


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Re: Using lids as petri dish [Re: lucidasans]
    #26671626 - 05/14/20 11:16 PM (3 years, 8 months ago)

Good luck! :thumbup:


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InvisibleApples in Mono
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Re: Using lids as petri dish [Re: LogicaL Chaos] * 1
    #26671899 - 05/15/20 03:52 AM (3 years, 8 months ago)

If you can master sterile technique with that, you'll be badass if you end up switching to normal plates


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Offlinelucidasans
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Re: Using lids as petri dish [Re: Apples in Mono]
    #26672152 - 05/15/20 08:12 AM (3 years, 8 months ago)

Quote:

Apples in Mono said:
If you can master sterile technique with that, you'll be badass if you end up switching to normal plates



It's kind of rustic, isn't? It's seems to be easier "no pour" technique. That's not an option to this method.

I dont know I'm going in a hard way trying lids like this. Wrap them after pour agar isnt inviable?

The easier method I found was those PP5 "no pour" like This.

Anxious to try. :rasta:


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Offlinerumfor69
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Re: Using lids as petri dish [Re: lucidasans]
    #26672188 - 05/15/20 08:35 AM (3 years, 8 months ago)

Also here is another great agar tek that may give you some other ideas

https://www.shroomery.org/forums/showflat.php/Number/19208976


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Offlinelucidasans
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Re: Using lids as petri dish [Re: rumfor69]
    #26672212 - 05/15/20 08:44 AM (3 years, 8 months ago)

Quote:

rumfor69 said:
Also here is another great agar tek that may give you some other ideas

https://www.shroomery.org/forums/showflat.php/Number/19208976



Thank you.

The TEK I posted is a "copy" of this one. Pastywhyte's TEK have more details, even better.


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OfflineA.k.aM
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Re: Using lids as petri dish [Re: lucidasans]
    #26672221 - 05/15/20 08:48 AM (3 years, 8 months ago)

This is actually a really good idea for people who can’t get plates easily.

I’d be kinda nervous letting them sit open while they hardened even in the SAB but you could just put foil over them I guess.


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