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OfflineNightPuma1
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How much growth do you actually need on an agar plate before transferring to grain?
    #26671051 - 05/14/20 06:55 PM (9 months, 19 days ago)

Since the point of agar is to ensure that you have clean grain, do you just have to wait until you can tell that there is no contamination or should you wait until there is a lot of growth on the plate?

Thanks.


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: NightPuma1]
    #26671090 - 05/14/20 07:19 PM (9 months, 19 days ago)

You want to transfer a few times to get clean growth, but you can also transfer sectors and let that grow out so your transferring organized growth. This will ensure more of the same genetic material in the jar. But as always with MS agar you can transfer 10000 times and still not get a true isolate, and even when you do it could end up being a poor fruiter or small shrooms or a ton of different variables.

So I like to run a T1 and T2 plate from the same MS transfer and then go about 4-6 times until I see healthy genetics. Them I will go to jars and grow it out to test the culture.


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OfflineLogicaL ChaosM
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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: NightPuma1]
    #26671147 - 05/14/20 07:47 PM (9 months, 19 days ago)

Quote:

NightPuma1 said:
Since the point of agar is to ensure that you have clean grain, do you just have to wait until you can tell that there is no contamination or should you wait until there is a lot of growth on the plate?

Thanks.




The point of agar is to ensure clean mycelium to put on grain. Its best to colonize a plate with clean mycelium so u can transfer all of it to grain or other agar plates. That would be the most efficient/cost effective way.


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: LogicaL Chaos]
    #26671219 - 05/14/20 08:38 PM (9 months, 19 days ago)

Yes but I'm asking how much growth do you need on your final dish before you transfer it to grain?

Just enough to make sure it is clean or should you let the whole dish grow out?

Thank you.


Edited by NightPuma1 (05/14/20 08:40 PM)


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: NightPuma1] * 1
    #26671225 - 05/14/20 08:44 PM (9 months, 19 days ago)

Thats up to you. Any growth will work but letting it grow out gives u more mycelium to work with.


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: LogicaL Chaos]
    #26671239 - 05/14/20 08:58 PM (9 months, 19 days ago)

Okay but I'm asking if it will affect how quickly the grain jar finishes or not.

Case 1: I let a small amount of mycelium grow on the agar until I can tell that it is not contaminated and then I transfer this to a grain jar as soon as possible.

Case 2: I let the entire petri dish finish colonizing and then transfer this to the grain jar.

Which would be better? In Case 1 you get the growth started on the grain earlier but it is a smaller amount of mycelium inoculating the grains, whereas in Case 2 you are starting later because you need to wait for the Petri dish to finish colonizing but you are dropping a larger amount of inoculating mycelium into the grain.

Hope that clarifies my question. Thank you.


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: NightPuma1]
    #26671255 - 05/14/20 09:09 PM (9 months, 19 days ago)

I see your issue now, your going for high-speed growth (need shrooms now protocols). I would go with Option 2, having more mycelium in the beginning would be an advantage in my opinion.

If u really wanna go high speed, cut your colonized agar into small pieces, drop into your master jar then after sealing the jar, shake well to mix up the spawn points to even faster speeds.

The fastest method is Liquid Cultures thou but its an extra step between agar and grain.


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: LogicaL Chaos]
    #26671295 - 05/14/20 09:42 PM (9 months, 19 days ago)

Okay one last question please.

So to isolate genetics you generally speaking want to slice off a hyphal node that has grown very far from the point of inoculation which implies that is an aggressive colonizer. Let's say that I do that several times and get one that is quite aggressive.

In your opinion, how often does it happen that you get some very aggressive growing mycelium that ends up being a poor fruit producer?

In other words, how frequently do you isolate some really aggressive strain "in petri" that does not hold up expectations when fruiting.

Thank you.


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: NightPuma1]
    #26671304 - 05/14/20 09:46 PM (9 months, 18 days ago)

That is a pretty advanced question that I dont know the answer to. Maybe try make a thread in Advanced Cultivation :shrug:

Typically thou, rhizomophic mycelium is a good choice for fruiting. Also, isolating strains is a very involved process. Are you ready for Advanced Growing Techniques right now?


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: LogicaL Chaos]
    #26671320 - 05/14/20 09:51 PM (9 months, 18 days ago)

I currently have some petri dishes with hyphal nodes sticking kind of far out and I am just wondering what the probability is after several transfers of ending up with a lousy harvest. I'm aware that it takes time and I'm going to be very patient, but if I have certain nodes sticking so far out it would be a little foolish not to try and potentially isolate some aggressive genetics. Just don't want it for nothing.


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: NightPuma1]
    #26671321 - 05/14/20 09:52 PM (9 months, 18 days ago)

Just pick the strongest, healthiest mycelium and it should be fine. When u get into cloning on agar, clone shrooms that are in clusters.


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: LogicaL Chaos]
    #26671331 - 05/14/20 09:56 PM (9 months, 18 days ago)

Thanks!


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Re: How much growth do you actually need on an agar plate before transferring to grain? [Re: NightPuma1] * 1
    #26671333 - 05/14/20 09:56 PM (9 months, 18 days ago)

Yeah it is still a crap shoot. Agressive mycelium doesn't translate into a good mushroom producer. The key to producing a good quality flush is more environment and growing conditions.


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