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OfflineAtomHeart
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Agar problem...opinions?
    #26670918 - 05/14/20 05:47 PM (9 months, 12 days ago)

Any idea what's up here?

Is this bacterial?  Never seen this kind of phenomenon...maybe the agar was made too wet?  It looks normal from underneath...but looks strange from up top.  Looks...moist.

If it's bacterial...it seems confined to the germination points...does that mean the spore print is contaminated?  If it were my inoculation loop it seems it would be everywhere I zig-zagged with the loop.  There's nothing odd happening where I cooled the loop in the agar after flaming..if it were the agar itself that was bacterial, it seems like it would be everywhere, and not just perfect patches exactly matching where the spores germinated.  Trying to figure out what's up so that if it's contamed I don't repeat it and don't transfer this to another dish.  Thanks for any help from experienced eyes.



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Edited by AtomHeart (05/14/20 06:03 PM)


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OfflineOmbisha
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Re: Agar problem...opinions? [Re: AtomHeart] * 1
    #26671910 - 05/15/20 06:05 AM (9 months, 11 days ago)

I don't know what this is and would like to. I had similar growth in my plates, but i transferred from the edge and then it was ok. At the time i thought it was bacteria riding along the myc strands.

Prints are always contaminated to some extent. Not sure if you know that.


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OfflineLogicaL ChaosM
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Re: Agar problem...opinions? [Re: AtomHeart]
    #26671918 - 05/15/20 06:21 AM (9 months, 11 days ago)

Interesting. Looks like transluscent wispy mycelium mixed with bacteria would be my guess.


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OfflineJe77Ce11ar
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Re: Agar problem...opinions? [Re: LogicaL Chaos] * 1
    #26671952 - 05/15/20 06:59 AM (9 months, 11 days ago)

how long ago did you put the spores to agar? if it was 5/9 it looks like your spores are just starting to germinate...give it a cpl more days, it should grow out more


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OfflineAtomHeart
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Re: Agar problem...opinions? [Re: Je77Ce11ar]
    #26672144 - 05/15/20 10:04 AM (9 months, 11 days ago)

Well, I was thinking some really bad bacteria riding along with the mycelium too...the agar is raised and moist-looking under the mycelium.

My first concern was that my agar batch didn't get sterilized well for some reason and was swarming with bacteria but my other plates that I 'nocced with other varieties appear to be ok so far.

I'll give it a few days and see if I get any white thick mycelium out around the edges of these raised patches that I can transfer.  I'd just toss it, but if the spore print is overrun with this bacteria, it's the only sporeprint of redboy I have left so I need to try to save it.

From the underside you can see mycelium that appears to be growing through the agar just under the surface or something.  In 25 years I've never seen this.  I'll keep my fingers crossed.

Thanks for the moral support guys.  I'll try to update on how this plays out.


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Edited by AtomHeart (05/15/20 10:05 AM)


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OfflineAtomHeart
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Re: Agar problem...opinions? [Re: AtomHeart]
    #26675103 - 05/16/20 05:19 PM (9 months, 10 days ago)

Hmm...this is what it has turned into...

I guess it's gonna be fine.  Still looks strange.



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Edited by AtomHeart (05/16/20 05:20 PM)


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Invisiblejbaby007
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Re: Agar problem...opinions? [Re: AtomHeart]
    #26675110 - 05/16/20 05:22 PM (9 months, 10 days ago)

Looks bacterial


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OfflineAtomHeart
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Re: Agar problem...opinions? [Re: jbaby007]
    #26675120 - 05/16/20 05:25 PM (9 months, 10 days ago)

I'm almost sure it is.  Wonder if I should try peroxidated agar or just start doing transfers and see if it shakes it. 

Feels like it's gonna be doomed when it gets into grain is my concern.


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You once ripped a man's jaw off...I seent it!



Edited by AtomHeart (05/16/20 06:02 PM)


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