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OfflineJohn in WI
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Senescence--How long does it take?
    #26670672 - 05/14/20 01:33 PM (3 years, 8 months ago)

I've finally overcome my several month battle with trich.  It was a serious drag!

I'm wondering now about senescence.  Since about August, I've been maintaining agar cultures of 3 strains.  I'm doing them in Ziploc storage containers with tyvek filter patches.  They are around 4" across.  As the mycellium fills the dish, I've been selecting the best looking agar and transfering it. Then using the agar to inoculate grain (1pt jars--then doing g2g from that). 

The point is, I haven't had any fruits since August, and just today was able to collect several very nice caps and set them up for printing.  I have them in my SAB, sitting on wooden skewers so they don't touch the aluminum foil, and covered with a beaker. IT should be very clean.

I'm wondering how long it takes for senescence to become a problem?  I've transfered the agar many times.  It seems to be growing vigorously when transfered to grain, but the fact is, these are from spores germinated a year ago.

I've been careful with the agar.  I never use the same recipe twice.  I switch it up--PDA, then grain soak water with yeast, then "everything agar" using sweet potato and turnip simmering water....  I'm hoping that varying the recipe would help keep the culture vibrant.  Able to keep all of it's enzymes working, so it can eat a variety of grain food sources.

Also, when innoculating grain, I've been varying the grain.  Sometimes I use oats, sometimes oats with maybe 1/4 popcorn. Sometimes WBS. 

So, how long does senescence take?  How do you know it's happening?  Is my approach--varying the grains and the carbohydrate profile in the agar a viable strategy to help prevent it? 

I'm considering, now with some fresh spore prints, starting over from scratch.  Innocluating agar with the new spores, selecting good rhyzomorphic mycellium.... 

Alternatively, if I clone one of the mushrooms, does this "reset the clock" on senescence? Or does the mature fruit "remember" that it is the result of year old mycellium?    I know with other clones--like Dolly the Sheep--her biology remembered.  While chronologically young, on a cellular level she was he age of her mom.


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OfflinePyroboy4206
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Registered: 04/30/17
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Re: Senescence--How long does it take? [Re: John in WI]
    #26805149 - 07/05/20 12:13 PM (3 years, 6 months ago)

These are good questions. I do not understand it either. In the way past I know I have been successful doing g2g at least 3 layers down over the course of three years. In the middle having the grain refrigerated.

What did I notice in those expirements? PE definitely lost the blobs! The real ambition of the fruit comes out when it is freshest. But alas, over that project... It worked.

-pyro in mke


Edited by Pyroboy4206 (07/05/20 12:14 PM)


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