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OfflineCalicoCap
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Overgrown lc, still good?
    #26669791 - 05/14/20 01:21 AM (3 years, 8 months ago)

Hey yall i have a lc that ive kept for a lonth now. I started it with a cc from another culture and it took off. My question is: can i still stir it up and use it? Or will the pins cause some rot within whichever jar it goes in? Thank you!



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Edited by CalicoCap (05/14/20 01:31 AM)


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Offlinethewaronthemind
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Re: Overgrown lc, still good? [Re: CalicoCap]
    #26669796 - 05/14/20 01:28 AM (3 years, 8 months ago)

The lc is pinning lol?


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OfflineCalicoCap
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Re: Overgrown lc, still good? [Re: thewaronthemind]
    #26669798 - 05/14/20 01:32 AM (3 years, 8 months ago)

Forgot to post the pics! Yeah man time got away from me and i forgot about this jar


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Offlinethewaronthemind
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Re: Overgrown lc, still good? [Re: CalicoCap]
    #26669805 - 05/14/20 01:37 AM (3 years, 8 months ago)

Wow that is so fucking cool those things just want to live!


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OfflineCalicoCap
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Re: Overgrown lc, still good? [Re: thewaronthemind]
    #26669812 - 05/14/20 01:43 AM (3 years, 8 months ago)

Part of me wants to let it grow and see how big it gets lol ide like to know what to do with it tbh. Its an awful lot to just pour out but i have more. I can also take a sample of it and expand it in a new jar.


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OfflineMugnath
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Re: Overgrown lc, still good? [Re: CalicoCap] * 1
    #26669814 - 05/14/20 01:44 AM (3 years, 8 months ago)

Rot will only occur if there are prior contaminations within the LC or bad sterile technique during inoculation. You can still stir and use, be wary of contamination however, run testers with that LC first (agar if you have it). LC is notorious for hiding contamination. In vitro pinning can occur on petri as well.


Edited by Mugnath (05/14/20 01:45 AM)


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OfflineCalicoCap
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Re: Overgrown lc, still good? [Re: Mugnath]
    #26669853 - 05/14/20 02:16 AM (3 years, 8 months ago)

Okie dokie! Ill test a bit on agar first!


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OfflineA.k.aM
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Re: Overgrown lc, still good? [Re: CalicoCap]
    #26670159 - 05/14/20 07:48 AM (3 years, 8 months ago)

Lol I was just telling gizmo about an old post like this where the guy had to big full grown shrooms on top of the lc.

I say shake it up and let the pins reabsorb.
(No idea if that would work)


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Offlinepoisoned
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Re: Overgrown lc, still good? [Re: A.k.a]
    #26670486 - 05/14/20 11:51 AM (3 years, 8 months ago)

I mean, how much different is that from cloning. I think it'd work.


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OfflineCalicoCap
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Re: Overgrown lc, still good? [Re: poisoned]
    #26671484 - 05/14/20 09:34 PM (3 years, 8 months ago)

Im down to try that actually! I started tnis lc from a spore syringes and expanded it twice, both times i took a cc from the jar to test and start it over. may be able to do thay again withing disturbing them. So i got enough mileage out of to where i dont mind trying TO GROW THE BIGGEST FRUIT I CAN within it.


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Invisiblesh4d0ws
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Re: Overgrown lc, still good? [Re: Mugnath]
    #26671535 - 05/14/20 09:50 PM (3 years, 8 months ago)

Quote:

Mugnath said:
Rot will only occur if there are prior contaminations within the LC or bad sterile technique during inoculation. You can still stir and use, be wary of contamination however, run testers with that LC first (agar if you have it). LC is notorious for hiding contamination. In vitro pinning can occur on petri as well.





I honestly don't know but are you sure about this? It seems like the mushroom goes through a life cycle eventually reaching a stage where it degrades/breaks down/"rots" and that this will occur because the mushroom is no longer living, occuring in a sterile environment as well (it can't live forever can it?)

Even in my cleanest agar dishes the pins always eventually break down or rot to some extent?

I guess we could be defining two different things but, I have a couple LC here that I got real lazy with and the pins and fruits it put out always turn mushy/rot. Even though I haven't opened the thing since inoculation (too much shit going on)


Maybe all my shit just has contamination in it already. Or we mean different things by rotting and decomposing.


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OfflineMugnath
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Re: Overgrown lc, still good? [Re: sh4d0ws]
    #26671552 - 05/14/20 10:09 PM (3 years, 8 months ago)

You are correct, rot will occur even without contams, I really didn't say that well :facepalm:. The mushrooms will naturally break themselves down.

What I was trying to get at, is from what I understand (which very well may be wrong), when that mushroom breaks down, it opens up another point for any still present contaminant spores to begin growth that may not have germinated yet.


Edited by Mugnath (05/14/20 10:21 PM)


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Offlinepoisoned
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Re: Overgrown lc, still good? [Re: Mugnath]
    #26671778 - 05/15/20 01:24 AM (3 years, 8 months ago)

Even if it "rots", which I don't think is the correct phrase here, it shouldn't be a contamination vector.


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OfflineA.k.aM
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Re: Overgrown lc, still good? [Re: poisoned]
    #26672059 - 05/15/20 06:57 AM (3 years, 8 months ago)

Yeah I think rot implies being broken down by microorganisms.

This would be more reverting back to myc.


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OfflineCalicoCap
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Re: Overgrown lc, still good? [Re: A.k.a]
    #26679628 - 05/18/20 06:51 PM (3 years, 8 months ago)

Heya, sorry i got busy the last few days. By rot i was more talking about reabsorbtion for sure. I forget that i have to be vert specific my bad. Ive got more pics! It looks like two colonies in the jar with the floater being the one pinning. I named them both Patricia or patty for short. Aside from being a little starved for fae they seem fine with the biggest fruit being a full inch long. There are new pins appearing as well. What do yall think? I havnt gotten around to sampling it yet and dont want to disturb whats going on either. Thanks for your interest and advice!
P.s. i am an oddball and like to keep the lights on in the colonizing area same as i do for my tubs. Could that have caused an overgrown lc to pin?



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