Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Offlinerobertplant
Stranger
 User Gallery
Registered: 12/21/19
Posts: 27
Last seen: 4 years, 28 days
First transfer wedge selection help
    #26668374 - 05/13/20 10:18 AM (4 years, 8 months ago)

I have finally succeeded transferring a tissue culture to agar!  It is now time to make some transfers in an effort to isolate.  I would love to hear your opinions on the best positions to target for taking wedges. 

Thanks ahead of time for any help!






Extras: Filter Print Post Top
OfflineOmbisha
Transmutant
I'm a teapot User Gallery


Registered: 02/17/20
Posts: 667
Loc: Tartaria
Last seen: 10 months, 2 days
Re: First transfer wedge selection help [Re: robertplant] * 1
    #26668397 - 05/13/20 10:32 AM (4 years, 8 months ago)

at this point, just pick a spot that you like and go for it. make the transfer as small as possible. I also only recently learned, that further isolation from a clone tissue might not desirable in most cases. You really just want to get a clean, nice looking plate, which you seem to have.


--------------------

Extras: Filter Print Post Top
OfflineOmbisha
Transmutant
I'm a teapot User Gallery


Registered: 02/17/20
Posts: 667
Loc: Tartaria
Last seen: 10 months, 2 days
Re: First transfer wedge selection help [Re: Ombisha]
    #26668402 - 05/13/20 10:36 AM (4 years, 8 months ago)

What i mean by "learned" is, that some TC wrote it in some thread. I haven't really looked into it further yet.


--------------------

Extras: Filter Print Post Top
OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 8 days, 14 hours
Re: First transfer wedge selection help [Re: Ombisha]
    #26668408 - 05/13/20 10:39 AM (4 years, 8 months ago)

The reason is that as you isolate more, you may be isolating traits you dont want.

If you have alot of different genetics then its really just a 100% clean ms inoculation.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

Extras: Filter Print Post Top
Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 2 years, 2 months
Re: First transfer wedge selection help [Re: The Mycologist]
    #26668445 - 05/13/20 11:05 AM (4 years, 8 months ago)

That almost looks like an isolate already. If you're set on getting a monoculture, you should probably wait a few more days to get some sectoring and then transfer whatever you feel is looking like a desirable growth.

I'd first make a transfer to another plate and a slant to keep your master that you can return to at any time.

Isolating is hard work with possibly a lot of undesirable results.


--------------------
How I do glass dishes

Extras: Filter Print Post Top
OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 8 days, 14 hours
Re: First transfer wedge selection help [Re: poisoned]
    #26668460 - 05/13/20 11:13 AM (4 years, 8 months ago)

Yea I say next plates are good for inoculating some grains as long as they look good still.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

Extras: Filter Print Post Top
Offlinerobertplant
Stranger
 User Gallery
Registered: 12/21/19
Posts: 27
Last seen: 4 years, 28 days
Re: First transfer wedge selection help [Re: The Mycologist]
    #26668917 - 05/13/20 04:47 PM (4 years, 8 months ago)

Thanks for the feedback. 

So further isolation is not really needed but I should still do one transfer just to ensure I have clean mycelium before going to grain?

Also, I don't have any slants, how long can I save a colonized agar dish in the refrigerator?

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Opinions about which agar plates should be transferred *DELETED*
( 1 2 all )
mr.s420 693 20 05/25/20 02:08 PM
by Domno
* transferring agar meatman 2,574 3 10/02/02 01:13 PM
by tripndicular
* G2G transfers...an endless cycle? ADoobie 7,509 18 11/12/20 07:10 PM
by FredsGotSlacks
* Agar to Liquid Culture. How much tissue to transfer? TheFish 1,705 3 02/12/08 10:10 AM
by TheFish
* transferring mycelium wikedanjel 4,251 13 01/10/03 03:54 AM
by debianlinux
* agar transfers to hpoo wutang 950 6 11/21/07 11:53 AM
by BreakfastClub
* Innoculation with agar wedge Visigoth 5,665 6 01/15/23 12:59 PM
by Screwup
* A small triangle or a large wedge? the_pigdog 754 4 09/02/06 11:31 PM
by creamcorn

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
108 topic views. 23 members, 139 guests and 20 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.008 seconds on 12 queries.