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Mycopsilo
Green Thumb



Registered: 04/07/20
Posts: 41
Loc: Under RI and MA
Last seen: 1 month, 28 days
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Thanks, I have gathered that much information Lmao.
-------------------- Grow it and they will come... If you want to view paradise, simply look around and view it..
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Mycopsilo
Green Thumb



Registered: 04/07/20
Posts: 41
Loc: Under RI and MA
Last seen: 1 month, 28 days
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Re: What is this Contam on my Agar? [Re: mushpunx]
#26656220 - 05/07/20 10:23 PM (3 years, 9 months ago) |
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Got it. Thanks. I will be updating my progress and posting pics as it goes.
-------------------- Grow it and they will come... If you want to view paradise, simply look around and view it..
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Mycopsilo
Green Thumb



Registered: 04/07/20
Posts: 41
Loc: Under RI and MA
Last seen: 1 month, 28 days
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Re: What is this Contam on my Agar? [Re: Mycopsilo] 1
#26667001 - 05/12/20 04:23 PM (3 years, 9 months ago) |
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Here is the B+ transfers. I wasn’t thinking when I did it and took the myc from the center... They are looking good though from what I can tell. The first plate I dropped received two pieces of agar by accident. A little sliver fell off of the main transfer. The second plate got a single piece. The second plate has a small dot of what looks like the start of some new myc?

Edited by Mycopsilo (05/12/20 04:31 PM)
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
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Re: What is this Contam on my Agar? [Re: Mycopsilo]
#26667940 - 05/13/20 04:37 AM (3 years, 9 months ago) |
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The second plate, that dot could either juSt be a piece of myc that fell off your scalpel or if your wedge rolled, and started growing.
But it also could be a mold colony - mold colonies start out as white spots like that, in places not touching your wedge or myc germination. That's why we swipe spores onto plates in zig zag patterns, so we have an idea where germination should be and don't take from outside of the pattern.
Plates like that I view as suspect, I take a transfer from the side furthest from the colony, before the myc touches it. That's what I would do.
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 Amateur Mycologists United AMU Q&A
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Mycopsilo
Green Thumb



Registered: 04/07/20
Posts: 41
Loc: Under RI and MA
Last seen: 1 month, 28 days
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Re: What is this Contam on my Agar? [Re: mushpunx]
#26668086 - 05/13/20 06:52 AM (3 years, 9 months ago) |
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Ok that’s what I’ll do. On a similar note I made some new Agar plates and did some swipes from my syringes. 5 more B+ and 5 PE. I poured the agar at 120F and I still got condensation.. What the hell is up with that? Every post I read said it starts to solidify at 120F but that wasn’t the case. So I noticed the agar was still liquified at 100F so I guess I will pour at 100 from now on. I don’t think My IR thermometer is off.
-------------------- Grow it and they will come... If you want to view paradise, simply look around and view it..
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
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Re: What is this Contam on my Agar? [Re: Mycopsilo]
#26668152 - 05/13/20 07:50 AM (3 years, 9 months ago) |
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You could go cooler if it's still causing problems. I eyeball mine a lot of the time, when it's just about going to solidify if I leave it any longer, I pour. Never get condensation.
I usually only do one or two germination plates of each variety because I'm just gonna looking to start one one culture of each. Saves plates
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 Amateur Mycologists United AMU Q&A
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Mycopsilo
Green Thumb



Registered: 04/07/20
Posts: 41
Loc: Under RI and MA
Last seen: 1 month, 28 days
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Re: What is this Contam on my Agar? [Re: mushpunx]
#26668198 - 05/13/20 08:19 AM (3 years, 9 months ago) |
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Ok. So you you just transfer off of those 2 plates to make more plates? How do you isolate? You just transfer off until you have a culture that is completely uniform?
-------------------- Grow it and they will come... If you want to view paradise, simply look around and view it..
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