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OfflineJaymyco
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Registered: 12/18/18
Posts: 23
Last seen: 2 years, 10 months
How does my agar look? * 1
    #26666912 - 05/12/20 03:41 PM (3 years, 8 months ago)

Lately I have gathered supplies to start a bulk grow . My first step was to make some agar plates which some I inoculated with spores and others with a liquid culture I have created almost a year ago. All the work was done inside a SAB.The plates were inoculated 11 days ago .Two of my agar plates were contaminated and I proceeded two days ago to clean the contamination on the one I inoculated with spores using a cotton swab dipped in peroxide in one plate and for the other that I inoculated with LC I took a scalpel removed the small dot of contamination which wasn't touching yet with the mycelium and after rubbed with peroxide.

Here are some pictures I took today :

1) The ones I inoculated with liquid culture (the one on the right is the one I removed the small piece of agar):











2 ) The ones I inoculated with the spore(the bottom left one is the one that had a yellow contamination in the edge of the plate on its left side) :
















So I'd like to know if they look okay if I have to fix something and if I can use them to inoculate rye grains!


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OfflineJwardoin
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Registered: 02/09/15
Posts: 135
Last seen: 1 year, 11 months
Re: How does my agar look? [Re: Jaymyco]
    #26666956 - 05/12/20 04:00 PM (3 years, 8 months ago)

These all look super bacterial to my eyes. The myc should be nice and uniform; all of the spots where it gets thin and you can see more of the agar color = no good. That said, I have never worked with LC, so I have no idea if those plates are supposed to look like that


Edited by Jwardoin (05/12/20 04:01 PM)


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OfflineTstone
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Registered: 10/16/18
Posts: 944
Loc: This Planet
Last seen: 6 days, 3 hours
Re: How does my agar look? [Re: Jwardoin]
    #26667016 - 05/12/20 04:31 PM (3 years, 8 months ago)

Definitely need more transfers, non of those plates are currently healthy for grains. In the future, you don't cut out the contamination off agar. You want to transfer a clean piece of myc onto  a new plate. The contamination could all over your plate, and only some is visible. None of those plates  are clean. 2-4 more transfers. I certainly wouldn't use any of  them, as is.

Your maz plates are in really bad shape, your going to have a really tough time working with those. You made a LC? If you think these plates are clean and ready for grain, your LC must be ladden with contamination.  There's some decent myc on the Costa Rican plates, that can be transferred and worked with. Maz I think are a lost cause.

Your entire agar procedure is way outta whack. Why a q-tip with perioxide exactly?

How did you make your LC a year ago?


Edited by Tstone (05/12/20 04:48 PM)


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