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Offlinespank0r
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Can you inoculate a rye grain jar with a spore syringe? * 1
    #26666754 - 05/12/20 02:38 PM (3 years, 8 months ago)

Hi,

I'm getting back into this hobby again.  I'm curious if one can simply inoculate a sterilized jar of rye grain with a 10ml spore syringe you get from any of the vendors.  I know a lot of pros use agar or other grain transfers which I won't be doing since I don't have the space.

I have done this many times with WBS and it *rarely* ever works.  Maybe rye is the way and if a syringe works just fine with maybe 1-2 CC's, great.

Thank you.


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Offlineouuwee
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Re: Can you inoculate a rye grain jar with a spore syringe? [Re: spank0r]
    #26666763 - 05/12/20 02:41 PM (3 years, 8 months ago)

Yes you can but compared to agar, clean g2g transfers or liquid culture, there's higher risk of contamination with spore syringes


Edited by ouuwee (05/12/20 02:42 PM)


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OfflineLogicaL ChaosM
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Re: Can you inoculate a rye grain jar with a spore syringe? [Re: spank0r]
    #26666764 - 05/12/20 02:42 PM (3 years, 8 months ago)

Its bad practice to inject spore solution onto grain because spore solution is not clean. Its much safer/smarter/better to put a drop on agar and then clean it up then drop a slice of agar in your grain jar.


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