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Offlinegabbk
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Registered: 03/06/20
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Is this orange spot a contaminant?
    #26664958 - 05/11/20 04:50 PM (3 years, 8 months ago)

Hi guys.


I can't really find the answer of what this orange spot underneath the tissue could be.
I've already had some other previous agars with this same phenomena, the same little area that -to me- seems to be just the agar getting discolored by the mycelium.
I would like to know if it really is what I'm guessing and if is okay to inoculate it to jars or I would be wasting my time.
Thanks in advance!

Btw: the agar recipe that I'm using by the moment is the classic LME but the malt extract instead of being the powdered version, I'm using the syrup one (here in my country that powder doesn't even exist lol)


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Edited by gabbk (05/11/20 05:05 PM)


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Offlinegabbk
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Re: Is this orange spot a contaminant? [Re: gabbk]
    #26665888 - 05/12/20 07:06 AM (3 years, 8 months ago)

Sorry to bump, but anyone? :confused::grin:


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InvisibleStoneTroegs
Mycopath
Registered: 11/04/18
Posts: 20
Re: Is this orange spot a contaminant? [Re: gabbk] * 1
    #26665903 - 05/12/20 07:17 AM (3 years, 8 months ago)

Hey. Only been doing agar for a bit now. It seems like the coloration is from the agar and potentially made more prominent by the green agar. Your myc looks good. I would go for another transfer to a different color dish and see what comes of it. Otherwise you could probably go to grain or whatever your next step is right from there.


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OfflineA.k.aM
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Re: Is this orange spot a contaminant? [Re: StoneTroegs] * 2
    #26665968 - 05/12/20 08:01 AM (3 years, 8 months ago)

It’s fine the myc will eat the food coloring

If you let it go long enough you can’t even tell the plate was colored at all.

Another cool thing is bacteria will suck it up too and turn bright colors so it’s real easy to see.


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LAGM2020


Edited by A.k.a (05/12/20 08:03 AM)


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