Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Offlinepattycakes77
Noobie
Male User Gallery

Registered: 04/28/20
Posts: 19
Last seen: 1 year, 7 months
Pretty sure it got contaminated :( * 1
    #26664846 - 05/11/20 04:03 PM (3 years, 9 months ago)

So I few weeks ago I posted some pics from my BRF Jars of Brazil Corumba mycelium and was a little worried because there were some grey-ish areas on the Jar around the edges of the white mycelium.  So in a way this is kind of an update as well as curious if anyone else has had this happen to them. 

I went a head and let them continue to colonize because some people mentioned that they have had grey looking mycelium and their grows turned out fine.  So today I birthed a few of them just to get a better look.  I have 10 jars and all look pretty much the same. As far as procedure goes, I used 2 to 1 ratio verm to brown rice flour and water, brought to field cap and PC on 15 psi for 90 min.  flame sterilized between each jar and inoculated the next day.  I tried to be as sterile as possible, but I didnt have a flow hood or a SAB at the time.

Its also worth noting that is is my first ever attempt to grow, so the inoculation didnt go so smooth, lol.  I ended up putting too much liquid culture in my jars, and had some trouble properly inoculating between the substrate and jar.  blame it on first time excitement I guess.  Other than that, I may of fucked up by putting regular tape over the injection holes.  Not sure if that contributes to the off color.

Anyways, I birthed two cakes, they came out easy and feel spongy to the touch, but firm.  The cakes smell like fresh mushrooms as well.  Has this happened to anyone before?  Wondering if anyone knows what this is as far as contam, if it is that. 



Extras: Filter Print Post Top
OfflineMushit
Shit Lover


Registered: 02/04/04
Posts: 253
Loc: The Great White North
Last seen: 8 months, 4 hours
Re: Pretty sure it got contaminated :( [Re: pattycakes77]
    #26664853 - 05/11/20 04:06 PM (3 years, 9 months ago)

Yep.  That sure looks like trich.
Don't be spreading that shit around.  Future grows could suffer.


--------------------
Up to my elbows in shit!


Extras: Filter Print Post Top
Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
Re: Pretty sure it got contaminated :( [Re: Mushit] * 1
    #26664861 - 05/11/20 04:14 PM (3 years, 9 months ago)

Not trich, but yeah, it's mold


--------------------
How I do glass dishes


Extras: Filter Print Post Top
OfflineMushit
Shit Lover


Registered: 02/04/04
Posts: 253
Loc: The Great White North
Last seen: 8 months, 4 hours
Re: Pretty sure it got contaminated :( [Re: poisoned]
    #26664870 - 05/11/20 04:23 PM (3 years, 9 months ago)

Do you know what it is specifically, poisoned?


--------------------
Up to my elbows in shit!


Extras: Filter Print Post Top
Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
Re: Pretty sure it got contaminated :( [Re: Mushit]
    #26664874 - 05/11/20 04:24 PM (3 years, 9 months ago)

No idea, but trich doesn't look like that.


--------------------
How I do glass dishes


Extras: Filter Print Post Top
Offlinepattycakes77
Noobie
Male User Gallery

Registered: 04/28/20
Posts: 19
Last seen: 1 year, 7 months
Re: Pretty sure it got contaminated :( [Re: poisoned]
    #26664908 - 05/11/20 04:38 PM (3 years, 9 months ago)

I'm wondering if it's a form of aspergillus mold.  It's pretty common in house holds and can be grey or green on color


Extras: Filter Print Post Top
InvisibleModularMind
M.P.F.
Male User Gallery


Registered: 02/09/10
Posts: 7,902
Re: Pretty sure it got contaminated :( [Re: pattycakes77]
    #26664950 - 05/11/20 04:44 PM (3 years, 9 months ago)

How did you source you liquid culture?


Extras: Filter Print Post Top
Offlinepattycakes77
Noobie
Male User Gallery

Registered: 04/28/20
Posts: 19
Last seen: 1 year, 7 months
Re: Pretty sure it got contaminated :( [Re: ModularMind]
    #26665007 - 05/11/20 05:22 PM (3 years, 9 months ago)

I didn't make it.  I procured it from a vendor. Also, looking back I was pretty certain I was sterile as possible.  Someone mentioned it could of been the syringe that I got, and I'm thinking it was.  A week after I inoculated the Corumba, I did a batch of Golden Teachers using the same tek.  The GT are doing really well right now.  But at the time I had 1 extra jar of BRF leftover, so I grabbed the Corumba syringe that had a few CC left and decided to inoc the extra jar.  I put a new needle on, wiped the body of the syringe down with alcohol, and flame sterile the needle.  I just noticed that the extra jar has the same mold growing in it as well.


Extras: Filter Print Post Top
InvisibleModularMind
M.P.F.
Male User Gallery


Registered: 02/09/10
Posts: 7,902
Re: Pretty sure it got contaminated :( [Re: pattycakes77]
    #26665018 - 05/11/20 05:29 PM (3 years, 9 months ago)

Just for my curiosity was it..

A: White mycelium suspended in water?

or

B: Black spores suspended in water?


Extras: Filter Print Post Top
Offlinepattycakes77
Noobie
Male User Gallery

Registered: 04/28/20
Posts: 19
Last seen: 1 year, 7 months
Re: Pretty sure it got contaminated :( [Re: ModularMind]
    #26665048 - 05/11/20 05:50 PM (3 years, 9 months ago)

Quote:

ModularMind said:
Just for my curiosity was it..

A: White mycelium suspended in water?

or

B: Black spores suspended in water?






It was black spores suspended in water


Extras: Filter Print Post Top
OfflineJustweed
Bobby's short shorts
 User Gallery


Registered: 04/01/20
Posts: 555
Last seen: 4 months, 5 days
Re: Pretty sure it got contaminated :( [Re: ModularMind]
    #26665082 - 05/11/20 06:12 PM (3 years, 9 months ago)

Definitely sounds like a contam from the source material. Usually if every jar has a contam like that and you followed relatively sterile procedure, especially with PF, that's what it is. Syringes aren't too difficult really if they're prepared for you. Flame the tip (which you did) and wipe the lid with alcohol sometime before injecting, then cover (which you did).

That's 100% mold of some sort, I'd kill it with fire. Toss it asap. Sterilize your area that it was in. If you put it in a fruiting chamber, I'd toss any perlite or verm that was in there and bleach down the chamber before putting anything else in there.


--------------------


Keep 'em high and tight guys....


Extras: Filter Print Post Top
Offlinepattycakes77
Noobie
Male User Gallery

Registered: 04/28/20
Posts: 19
Last seen: 1 year, 7 months
Re: Pretty sure it got contaminated :( [Re: Justweed]
    #26665225 - 05/11/20 07:25 PM (3 years, 9 months ago)

Oh yea for sure!  I already tossed my cakes, washed the jars and soaked them in bleach water solution.  I'm actually PC'in them right now just to make sure.  I know its overkill, but I plan in using them with my next syringe in a few days, so better safe than sorry.  Luckily I didn't put them in my fruiting chamber, and I sprayed the shit out of the tub I had my jars in and the area I work in when doing inocs and growing.  I need more iso alcohol after today! Lol.  That shit is getting hard to find lately.


Extras: Filter Print Post Top
InvisibleModularMind
M.P.F.
Male User Gallery


Registered: 02/09/10
Posts: 7,902
Re: Pretty sure it got contaminated :( [Re: pattycakes77]
    #26665597 - 05/11/20 10:55 PM (3 years, 9 months ago)

Quote:

pattycakes77 said:
Quote:

ModularMind said:
Just for my curiosity was it..

A: White mycelium suspended in water?

or

B: Black spores suspended in water?






It was black spores suspended in water




So, for future reference, you have a multi-spore (MS) syringe of ungerminated spores in a non-nutritive solution.

A liquid culture is currently living eating and growing.

Is the vendor replacing them for you?


Extras: Filter Print Post Top
Offlinepattycakes77
Noobie
Male User Gallery

Registered: 04/28/20
Posts: 19
Last seen: 1 year, 7 months
Re: Pretty sure it got contaminated :( [Re: ModularMind]
    #26665930 - 05/12/20 07:40 AM (3 years, 9 months ago)

Oh cool! Thanks for the info, I appreciate it. I'm still learning my terminology with all this fun new hobby.  But I haven't contacted the vendor about it. Is that something they even do?  I mean, given what we do with it and all.


Extras: Filter Print Post Top
InvisibleModularMind
M.P.F.
Male User Gallery


Registered: 02/09/10
Posts: 7,902
Re: Pretty sure it got contaminated :( [Re: pattycakes77] * 1
    #26666232 - 05/12/20 10:28 AM (3 years, 9 months ago)

Just say you scoped it out and it’s contaminated with mold spores.
It depends on the vendor and how they value their reputation/reviews.
Are you considering agar?
You can steam sterilize no pour plates. It’s easy.


Extras: Filter Print Post Top
Offlinepattycakes77
Noobie
Male User Gallery

Registered: 04/28/20
Posts: 19
Last seen: 1 year, 7 months
Re: Pretty sure it got contaminated :( [Re: ModularMind]
    #26666401 - 05/12/20 11:57 AM (3 years, 9 months ago)

Yes! I actually have just bought everything I need for agar and should get everything by next week.  I'm pretty excited.  Bought a neat SAB too.  Didn't want to make one and didn't mind buying a good one online.  And I'll give it a shot with the vendor and see if they will accommodate me.  What do you mean by no pour plates?  I did buy the malt extract and agar to make/pour plates


Extras: Filter Print Post Top
InvisibleModularMind
M.P.F.
Male User Gallery


Registered: 02/09/10
Posts: 7,902
Re: Pretty sure it got contaminated :( [Re: pattycakes77] * 1
    #26667232 - 05/12/20 06:42 PM (3 years, 9 months ago)

If you have an SAB, media bottles, petris, and agar just follow a traditional tek and search for no-pour later. Traditional petris are your best option for recognizing potential contamination and sectoring away the best mycelium.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Contaminated syringes? houshroom 1,376 16 08/15/03 04:35 PM
by OJK
* Dogs, cats and contamination? Dexter666 1,795 8 02/14/04 01:49 PM
by Deviant
* How to get rid of sources of contamination?
( 1 2 all )
LukeTheDuke187 1,336 26 07/28/21 10:54 AM
by LukeTheDuke187
* Possible Contaminated Spore Syringe? Tim2133 2,929 5 06/30/03 11:09 PM
by Sev
* Pins on agar and one with contaminant TripPSmittens 1,716 10 05/07/21 09:45 AM
by TripPSmittens
* Recognizing and dealing with contamination
( 1 2 3 4 ... 262 263 )
Kizzle 427,744 5,240 02/12/24 06:50 AM
by RockinRobot
* Contamination in karo jar EnlilMDiscord 1,935 11 09/27/03 02:30 PM
by Rose
* Can contaminated spore water be saved? mesq 2,749 5 03/15/02 01:24 PM
by rungi

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
223 topic views. 29 members, 154 guests and 32 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.006 seconds on 12 queries.