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OfflineCogu Melo
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Cogu Melo Grow Diary [agars looking better, liquid culture and brf jars waiting]
    #26664069 - 05/11/20 08:33 AM (3 years, 9 months ago)

Hello to all the partners in this adventure of mushroom learning, today i decide to open a thread to have some assistance in discovering and solving issues i may encounter in my journey. Its not my very beginning, im in this journey for basically one month and half, that talking on putting hands on work, had read alot about it since years i become interested. By now i had done some transfers, had inclusive some success in some of the agar i worked, getting full colonized without apparently contamination and by that transfer it to grain, will not post it all because i want to start the thread simple and show the progress from those ones im cleaning.

Somehow i feel a bit dis-motivated with my rate of success and want to learn more of what is happening in mine agars, i had not been that serious on reading threads integral and made some issues, for example was using very big transfers from colonized plates thinking it will colonize faster, not thinking on the bigger chance to contaminate them.

As you will see i had some problems with the pouring, i get this potato pre-made bought agar getting to dense after sterilize and it stays to solid, i had measure for 35º to put them but somehow the temperature of agar stays different from top to botton and when i pour them was already to much solid... Had previously done some other plates with pre-made malt extract agar, didnt get this problem only thing i change was the bottle, before i use jars of 250ml to do agar, now i had used tomato paste glass bottle 500ml and dont know whats happened, maybe something about what i had said before, but had ordered already media bottles, light malt extract and agar, have 200 pre-sterilized plates ready to use so i think it will turn a big thread, keep an eye on it i will be glad for all that stay around. :wink:

Those are the 3 agars that i had try to clean yesterday:

NUMBER ONE: MAZAPATEC (3 TRANSFERS)


NUMBER TWO: BE POSITIVE (2 TRANSFERS)


NUMBER THREE: GOLDEN TEACHER (1 TRANSFER)


EXTRA PLATE A:
(i didnt did nothing with this plate, this also have bacteria right? :frown: )



Beside that i have a problem with pressure cooker, right now i have one very small, 8 litters, that reach 0.8 bar/11 psi, had been sterilize agars for 35 minutes, i plan to buy another PC but I'm in Europe and its not being easy. Had been looking to buy a presto 23 quart, i know its very hard to find on Europe so im trying to find the best deal in us, the problem is all the shit that is cheap is out of stock its incredible even the official store of them dont have stock so i dont know what to do because with import and shipping the less i get deals right now are like 300€+, this including shipping and import charges, any kind of advice from people on Europe? Had look at this my.us that accept orders from us and ship it to Europe, it was a option but cant find good deals right now :| by now im really thinking in buying this one, what do you guys think?
https://www.ebay.com/itm/Commercial-18L-Steam-Autoclave-Sterilizer-Tattoo-Dental-Medical-Sterilization/143598903790?epid=23026728041&hash=item216f2961ee:g:iCkAAOSw91JcBour

Also other problem im having is to find a infrared temperature pistol, had search around but most of them are out of stock because of this virus i guess, what i can find is like 30€ more, isn't that too expensive thinking I'm up to order from china? There is other ways maybe cheaper also to do accurate temperature check?


Edited by Cogu Melo (06/08/20 02:57 PM)


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OfflineA.k.aM
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Re: Cogu Melo the Beginning [Re: Cogu Melo]
    #26664079 - 05/11/20 08:43 AM (3 years, 9 months ago)

Those are all super dirty.

Either you need to learn SAB technique or the agar isn’t sterile.

I’d pc an hour next time if it only goes to 11psi. Might be overkill but won’t hurt.


It’s gonna be very difficult to clean those with all that mold sporulating in there.

The gt looks dead but some of the other myc has nice growth, it’s just too bad there’s so much other stuff with it.

Good luck man everything you need is here, but it’s a lot of reading.

I’m always super impressed with you guys who get through all this with English as a second language.


--------------------
LAGM2020


Edited by A.k.a (05/11/20 08:46 AM)


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OfflineCogu Melo
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Re: Cogu Melo the Beginning [Re: A.k.a]
    #26664177 - 05/11/20 10:10 AM (3 years, 9 months ago)

Thanks for fast answer, . I tried to pick just a little bit of each of those lets see how it will be going, will make updates on the plates in some days. I think it should be on the transfer process because i poured other agars to late transfer that i didn't colonize and they don't appear to have any contamination... The problem I had already recognize was that i was putting huge transfers from contaminated plates, so this ones what changed was that I had pick the smallest transfer that i could, lets see if they have hypotheses...

I use a SAB already and i try to be as clean as possible with mask, gloves and alcohol swiping and after soapy spray with a pinch of bleach, the problem i find in mine is that it's too small so im gonna build another soon.

This last Mazapatec plate I posted, is it bacteria on the sides or it could be just a reaction of the agar or something? This one was one of my best hopes. :frown:


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OfflineCogu Melo
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Re: Cogu Melo the Beginning [Re: Cogu Melo]
    #26665611 - 05/11/20 11:07 PM (3 years, 9 months ago)

this is one of the 4 ryes that i had inoculate with one agar, b+ at the day 29 of last month, what your opinions on those ones? contaminated or i can have some hope on those ones? :rolleyes:



Edited by Cogu Melo (06/02/20 11:25 AM)


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InvisibleNeomorph
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Re: Cogu Melo the Beginning [Re: Cogu Melo]
    #26665697 - 05/12/20 01:53 AM (3 years, 9 months ago)

I start pouring agar at 40 C becouse at 37 it will start to go solid. And you will need at least dubble the time to PC everything until you get a good PC.

The termometer you will need for pasturization, you need to buy meat thermometer, it will masure inside of the substrat better then pistol one.

Grains should be colonised within 2 weeks max, but give them a shake if the turn white within 24-48 hrs its worth giving a try, if not toss them cuz you can make stuff harder for next growth.

Keep trying and you will suceed :mushdance:


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Offlinepoisoned
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Re: Cogu Melo the Beginning [Re: Neomorph]
    #26665790 - 05/12/20 05:14 AM (3 years, 9 months ago)

Quote:

Cogu Melo said:
Beside that i have a problem with pressure cooker, right now i have one very small, 8 litters, that reach 0.8 bar/11 psi, had been sterilize agars for 35 minutes, i plan to buy another PC but I'm in Europe and its not being easy. Had been looking to buy a presto 23 quart, i know its very hard to find on Europe so im trying to find the best deal in us, the problem is all the shit that is cheap is out of stock its incredible even the official store of them dont have stock so i dont know what to do because with import and shipping the less i get deals right now are like 300€+, this including shipping and import charges, any kind of advice from people on Europe? Had look at this my.us that accept orders from us and ship it to Europe, it was a option but cant find good deals right now :| by now im really thinking in buying this one, what do you guys think?

Also other problem im having is to find a infrared temperature pistol, had search around but most of them are out of stock because of this virus i guess, what i can find is like 30€ more, isn't that too expensive thinking I'm up to order from china? There is other ways maybe cheaper also to do accurate temperature check?




I'd try working with your current PC for now, used to work with one like that and you can definitely make it work. It's not great, but just before the current situation, I got one on Amazon. Including import taxes and shipping it was 150€. I know some people got it for 120€ even.

Regarding the infrared thermometer, you don't really need it. I got mine from Lidl last year.

Quote:

A.k.a said:
I’d pc an hour next time if it only goes to 11psi. Might be overkill but won’t hurt.

I’m always super impressed with you guys who get through all this with English as a second language.




30mins worked for me with that kind of a PC. Just make sure to vent it well before you start your timer.

Language barrier is not the biggest issue here, at least for me. It's the lack of canning resources that makes mycology hard here in Europe. You either have to work around it with what you have and tweak the teks, hoping for the best or you got to import everything, which isn't cheap. Another source of confusion is that Americans love to call their stuff by the popular brand names, so it sometimes takes a lot of time to find your local equivalent of the same product.

I should pull out my small PC again and write some beginners European teks.

Quote:

Cogu Melo said:
The termometer you will need for pasturization, you need to buy meat thermometer, it will masure inside of the substrat better then pistol one.

Grains should be colonised within 2 weeks max, but give them a shake if the turn white within 24-48 hrs its worth giving a try, if not toss them cuz you can make stuff harder for next growth.

Keep trying and you will suceed :mushdance:



I think he wanted to measure the PC temperature. Most people don't pasteurise anything these days. Grains can take longer if your prep is wrong and for beginners it often is. Hell, I hadn't been growing for a year and started with way too dry grains this time, wondering what is happening to my jars.

That grain jar is bad tho. There's mostly mold growing with some myc inbetween.

I wouldn't really blame those mishaps on your PC, I think you're doing something wrong when pouring those plates.


--------------------
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OfflineCogu Melo
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Re: Cogu Melo the Beginning [Re: poisoned]
    #26666831 - 05/12/20 03:08 PM (3 years, 9 months ago)

I think alot of my problem was already in the environment, i leave in old house and in the place where i was putting my sab there is lot of green fungus on the walls especially close to one window so i suspect the place was full of spores, before i didnt inspect that much but after reading about this green stuff was searching and found the contamination's could come from the air. I use still airbox but i think it was not enough. Other problem was i was doing wrong with the transfers, basically was picking too big pieces of contaminated plates so it was more probable that i would bring also contaminations together...

Right now i have different room, i pass bleach on all the walls and windows even a bit of outside so i think its alot cleaner around right now and also had done the transfers with just a pinch of what i thinked was healthy mycelium, today had made more 4 transfers, two from the plate I will post picture and two from grains of a growkit that is in third flush already.

Yes for sure im doing something wrong with pouring agar, first of all was temperature, will control it better next time and but had read abit and next plates should be alot better i hope, im just hoping to have some clean transfers to have more to work about...

Right now we have 5 sources and 10 new plates.
NUMBER ONE: (3 transfers)
NUMBER TWO: (2 transfers)
NUMBER THREE: (1 transfer)
NUMBER FOUR: (2 transfers)
GROWKIT FIVE: (2 transfers)

this one is the NUMBER 4, the one that looks more clean of those transfers i made:


In some days will post updates on all of those...


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OfflineA.k.aM
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Re: Cogu Melo the Beginning [Re: Cogu Melo] * 1
    #26666844 - 05/12/20 03:14 PM (3 years, 9 months ago)

That’s a much better size transfer.

Hopefully the plate stays clean cuz that’s nice mycelium.


When you take transfers make sure there’s no contamination close by. It’s probably ok but that bacteria is pretty close to where you cut.


--------------------
LAGM2020


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OfflineCogu Melo
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Re: Cogu Melo the Beginning [Re: A.k.a]
    #26670902 - 05/14/20 03:36 PM (3 years, 8 months ago)

okay making updates of what's going on, basically today after the 4 flush of a growkit, that growkit is father of the b+'s agars, im thinking in smashing the mycelium into two boxes and spread a mixture of 2 vermiculite to 1 of peat moss with a tea spoon of gypsum. I dont have the lime by now but as i was doing new agar I make this mixture and put it together with the agar for 45 minutes in pressure cooker. if its okay tomorrow will proceed, if no answer will just do another dunk and flush it again...

hope this agars will work better i think so, had seen d3monic agar technique and implement some stuff like use the blender for some time to make it all 1, i think this improve the way it will look because before it stays too much thick in some parts also because the thing not dissolved very well... will not colonize any by today will just poor them, tomorrow or maybe after tomorrow will post updates of the 10 transfers i made this week.

d3monic agar thread: https://www.shroomery.org/forums/showflat.php/Number/26628234#26628234

also someone is selling this pressure cooker for very cheap close to my city, 25€ so as i'm looking as crazy for the presto 23 and its all out of stock or at ridiculous prices and as i need to master agar first than grain maybe was good invest or not? it have 8 litters...


Edited by Cogu Melo (05/30/20 07:04 AM)


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Offlinepoisoned
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Re: Cogu Melo the Beginning [Re: Cogu Melo]
    #26670907 - 05/14/20 03:38 PM (3 years, 8 months ago)

That looks more like a convection oven


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OfflineCogu Melo
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Re: Cogu Melo the Beginning [Re: poisoned]
    #26670940 - 05/14/20 04:00 PM (3 years, 8 months ago)

yeh it looks, its strange how it operates but its a pressure cooker, at least its what they advertise... other picture:



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OfflineA.k.aM
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Re: Cogu Melo the Beginning [Re: Cogu Melo]
    #26670961 - 05/14/20 04:12 PM (3 years, 8 months ago)

Huh yeah that looks like a pressure cooker alright.

Weird top attatchment


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Offlinepoisoned
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Re: Cogu Melo the Beginning [Re: A.k.a]
    #26671016 - 05/14/20 04:35 PM (3 years, 8 months ago)

I mean, those temperature settings don't make sense for a cooker.


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OfflineCogu Melo
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Re: Cogu Melo the Beginning [Re: poisoned]
    #26681479 - 05/19/20 04:37 PM (3 years, 8 months ago)

hey fellows, will post some updates, by now there is nothing that is that promising but I found a pressure cooker that can reach 15 psi here in Europe, so i bought it because i just cant find any presto 23 quarts on stock for good price so will learn with this one as the situation normalize, than i can resell it for good price compared to what i paid. 50 euros for a Magefesa practika plus 8 quarts so quality of next sterilizations should be shorter and better. here is my new friend (:



"Pressure control system with 2 cooking levels fast cooking -8 PSI-, super fast cooking -15 PSI"


Here is the agar that looks better from those transfer's that i post before, think its looking good am i right, its not very dense but looks healthy or not?



Other question, is those filters any good? thinking in buying sfd's from us but they are really expensive with the shipping included, always same problem...



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OfflineCogu Melo
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Re: Cogu Melo the Beginning [Re: Cogu Melo]
    #26704363 - 05/29/20 11:31 AM (3 years, 8 months ago)

hello again, I'm coming with good news, after having this pressure cooker i made some new agar pours and it had gone much better than the previous tries, I had use this last agar i had put photo in here to make some transfers from different areas, some had bacteria (more on the edges) but some picks i choose from more in the middle are going as i can see perfect, i will be out for maybe 3 days and i still have 6 plates that i poured but didn't inoculate, do you think i should choose one of those 3 and make some transfers today? or should i wait them to colonize more and make the transfers after i return? here are the pictures:

number one:


number 2:


number 3:


number 4: what the problem with this one?


I tried to germinate also some spores from b+, as the cultures i had was getting harder to clean and also old so i choose to start fresh with a spore i made from some fruits i picked last month.

b+ spores:


today arrived the media bottles of 500ml and with time im improving my tools and materials, still have some orders to arrive and soon all this will flow better i hope.


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OfflineCogu Melo
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Re: Cogu Melo the Beginning [Re: Cogu Melo]
    #26706459 - 05/30/20 05:40 AM (3 years, 8 months ago)

alright i just finish making 6 new transfers right now, all from the number 3, will post updates later in some days, hope they all stay clean, if so will have a lot to work with, wish me luck :crazy2:


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OfflineCogu Melo
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Re: Cogu Melo the Beginning [Re: Cogu Melo]
    #26713707 - 06/02/20 10:44 AM (3 years, 8 months ago)

Hello again, I'm coming to let you some updates of the plates I'm working with. Hopefully I can get some opinions of them. I'm thinking on doing liquid culture of the number 3, the one that i made some transfers of. This because I want to inoculate some brf jars as i'm just searching for some success having fruits and this technique looks the most forgivable. Here are the pics:

number one:


number two:


number three: (I made 6 transfers already of this one, and thinking in making liquid culture out of this)


number four: (b+ spores)


number five: (1 of the children of number three)


now i need some help with the liquid culture, i want to use this jar, it's just 100ml but i need just a bit and as fast as possible to colonize 4 jars, maybe 8. The problem is, I don't have a SFD or syringe filter, and I don't even have polyfill so what do you advise me to do? i'm a little bit blocked in how i should proceed :| here is the jar:



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OfflineA.k.aM
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Re: Cogu Melo the Beginning [Re: Cogu Melo]
    #26713739 - 06/02/20 10:58 AM (3 years, 8 months ago)

Good job you’ve gotten much better since your first plates you posted.

You don’t need anything special really to make lc. Just put the water and nutrients in and pc for 20 mins.

Then you can drop the wedge in using the SAB and when it’s done pour it into jars using the SAB.


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OfflineCogu Melo
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Re: Cogu Melo the Beginning [Re: A.k.a]
    #26714424 - 06/02/20 03:33 PM (3 years, 8 months ago)

the question that makes me worry is the lid build, i don't know what to do without having either polyfill, SFD's or syringe filter. Can i use just high temperature silicone and stay without breathing hole? thinking that i will be making just 100ml of it will that not be a problem?


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Re: Cogu Melo the Beginning [Re: Cogu Melo] * 1
    #26714503 - 06/02/20 04:00 PM (3 years, 8 months ago)

What would be the point of the silicone? To draw into a syringe? I'd say just use unmodified lid leave it a little loose when pcing then when ready to use either just pour it like aka said or take the lid off to draw into a syringe.
I like using syringe filters bit if you can't get any you can run unmodified lids.


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Edited by gizmo1 (06/02/20 04:02 PM)


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