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OfflineAbabyphoenix
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contam agar plate to suprise?
    #26663960 - 05/11/20 07:27 AM (3 years, 8 months ago)

Quick question:
I have a couple germination plates/transfer plates that have obvious mold or slime or whatever but also very obvious myc growth. If i hydrate some rye prep some coir throw it all together in some masons or something small can i get some suprises? I dont see why not, otherwise i'd just be throwing away some good myc. I wouldnt fruit inside cause the obvious mold but having a couple porch cups going? that could be cool! :crazy2: Im about to wash them out and prep more agar and PC run but i was thinking why not like at the bare minimum cut out a healthy chunk o' myc throw that outside in a potter or something?

Still tryin to get ahold of the whole agar process and now im at that point where i figure out what to do with old plates, any tips/recommendations are appreciated. Thanks :smile:


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:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
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Edited by Ababyphoenix (05/11/20 07:43 AM)


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InvisibleHtaeh
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Re: agar plate to suprise? [Re: Ababyphoenix]
    #26663966 - 05/11/20 07:30 AM (3 years, 8 months ago)

So is it out of the question to transfer the good myc to a new plate?


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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Offlinerumfor69
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Re: agar plate to suprise? [Re: Ababyphoenix]
    #26663968 - 05/11/20 07:30 AM (3 years, 8 months ago)

I personally would try to cut and transfer the healthy mycelium to another
agar plate and repeat the process until I had a clean culture.


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OfflineAbabyphoenix
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Re: agar plate to suprise? [Re: Htaeh]
    #26663974 - 05/11/20 07:32 AM (3 years, 8 months ago)

Quote:

rumfor69 said:
I personally would try to cut and transfer the healthy mycelium to another
agar plate and repeat the process until I had a clean culture.



Quote:

Htaeh said:
So is it out of the question to transfer the good myc to a new plate?




Oh yeah im already 1-2 transfers out from the plates im talking about on my way to the third :smile: Plates that arent good enough for grain spawn but still got some healthiness to them. I guess ill just wash them out and only mess with clean plates. I was just theorizing that's all

Im about to wash them out and prep more agar and PC run but i was thinking why not like at the bare minimum cut out a healthy chunk o' myc throw that outside in a potter or something?
Gotta make room for the new!


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:



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InvisibleHtaeh
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Re: agar plate to suprise? [Re: Ababyphoenix]
    #26663984 - 05/11/20 07:37 AM (3 years, 8 months ago)

To be fair to you though, I currently have a tub doing well which was spawned from grain nocc'd by a germination plate. It's entirely possible to noc up grain should you only introduce the healthy myc to the grain, albeit more risky to some degree. A risk I took with the germination plate was there were a lot of competing genetics growing upon one another and there could have been a contamination hidden beneath, however I believe this was not the case as so far so good, contamination free. If you wish to try then there is nothing stopping you, I did it for experience, an outside grow may not be an awful idea given you have noticeable contamination within said plates but you wont know unless you try.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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OfflineAbabyphoenix
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Re: agar plate to suprise? [Re: Htaeh]
    #26663988 - 05/11/20 07:41 AM (3 years, 8 months ago)

Quote:

Htaeh said:
To be fair to you though, I currently have a tub doing well which was spawned from grain nocc'd by a germination plate. It's entirely possible to noc up grain should you only introduce the healthy myc to the grain, albeit more risky to some degree. A risk I took with the germination plate was there were a lot of competing genetics growing upon one another and there could have been a contamination hidden beneath, however I believe this was not the case as so far so good, contamination free. If you wish to try then there is nothing stopping you, I did it for experience, an outside grow may not be an awful idea given you have noticeable contamination within said plates but you wont know unless you try.




DOesn't noccing from a germ plate negatate the whole reason of making a germ plate in the first place lol (transfering isloating genetics away from contams etc)? why not just go MS to grain at that point.  But i feel u, sometimes it just be like

I really want an outside thing to happen but i live in FL suburbs... Yeah. Might have to go out in the bush and start a myc patch


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:



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InvisibleHtaeh
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Re: agar plate to suprise? [Re: Ababyphoenix] * 1
    #26664012 - 05/11/20 07:58 AM (3 years, 8 months ago)

I understand what you mean but in practice, not really, through making a germination plate I can make clean transfers and before deciding to use that remaining myc for noccing up some grain (through lack of patience to wait for the transfers to grow out) I can eyeball it and determine whether its worth the time taking to try. Going ms to grain you have no opportunity to look at the myc. You also use more solution and in that you give more contams a chance to germinate.


Quote:

Ababyphoenix said:
I really want an outside thing to happen but i live in FL suburbs... Yeah. Might have to go out in the bush and start a myc patch




Savage lol but yeah go for it, why not? I live in the UK and haven't a real hope in hell in getting any dodgy spawn to grow outside yet that doesn't stop me throwing it under some organic soil in the shade.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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