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Offlinelittlemushroom
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Registered: 04/30/20
Posts: 32
Last seen: 4 years, 4 months
Agar infected again
    #26663641 - 05/11/20 12:25 AM (4 years, 8 months ago)

Hi all
I made agar culture but it infected.I tried to save healthy mycelium but i always made a mistake. When cut the healthy mycelium and put it on the new agar, i infect new agar again. I work in still air box. I always disinfect the blade and the box and my hands. I don't know what a'm doing wrong. Today i have made new agar and saved mycelium. I hope it will be succesfull.
What do you think could be the mistake?

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OfflineMH5109
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Registered: 09/27/17
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Re: Agar infected again [Re: littlemushroom]
    #26663650 - 05/11/20 12:50 AM (4 years, 8 months ago)

Try using a syringe, and take a biopsy. Pc the syringe with some water in it and then take your biopsy and put a drop or two of water on the receiving plate. What type of contamination are you getting, bacteria, mold, yeast.


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Offlinemushpunx
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Registered: 04/20/14
Posts: 13,394
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Re: Agar infected again [Re: MH5109]
    #26663743 - 05/11/20 03:29 AM (4 years, 8 months ago)

You are flame sterilizing your scalpel, correct? That's what you mean by "disinfect"?

Just keep trying, you'll get it. Post some photos for us to help


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Amateur Mycologists United
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Offlinelittlemushroom
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Registered: 04/30/20
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Last seen: 4 years, 4 months
Re: Agar infected again [Re: mushpunx]
    #26665889 - 05/12/20 07:07 AM (4 years, 8 months ago)

Yes i always use flame to sterilize my blade.
The contaminations are yellow bacteria  and a bit white and transparet thing. I dont know these.  In the evening I try to take a good picture

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InvisibleStoneTroegs
Mycopath
Registered: 11/04/18
Posts: 20
Re: Agar infected again [Re: littlemushroom]
    #26665898 - 05/12/20 07:14 AM (4 years, 8 months ago)

When you’re working in the SAB do you have any windows open or fans going? Your air may not be as still as it appears. I also use Ozium spray before any SAB work.
On that note. I’ve been getting a lot of what seems similar. Starts out white but fluffy. I usually think it’s just tomentose myc until it starts to take on a yellow/ orange tint. Almost what it looks like when you singe the end of your hair. Idk what it is. Only happens when I use SS that are older. I’m currently working on trying to transfer it out. Let’s see.

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Offlinepoisoned
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Registered: 04/17/13
Posts: 1,738
Loc: Yurop
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Re: Agar infected again [Re: StoneTroegs]
    #26666009 - 05/12/20 08:22 AM (4 years, 8 months ago)

You don't wipe the scalpel after flame sterilization, right?


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Invisiblegoatchild
mr noob


Registered: 10/31/19
Posts: 162
Re: Agar infected again [Re: littlemushroom]
    #26666080 - 05/12/20 09:01 AM (4 years, 8 months ago)

Are you using petri dishes? Do they come pre-sterilized? If so are you opening the plastic wrap inside the SAB?


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"What stands in the way becomes the way."

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