Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Offlinelittlemushroom
Stranger
Registered: 04/30/20
Posts: 32
Last seen: 3 years, 4 months
Agar infected again
    #26663641 - 05/11/20 12:25 AM (3 years, 8 months ago)

Hi all
I made agar culture but it infected.I tried to save healthy mycelium but i always made a mistake. When cut the healthy mycelium and put it on the new agar, i infect new agar again. I work in still air box. I always disinfect the blade and the box and my hands. I don't know what a'm doing wrong. Today i have made new agar and saved mycelium. I hope it will be succesfull.
What do you think could be the mistake?


Extras: Filter Print Post Top
OfflineMH5109
Stranger
 User Gallery

Registered: 09/27/17
Posts: 1,385
Loc: America Flag
Last seen: 2 years, 11 months
Re: Agar infected again [Re: littlemushroom]
    #26663650 - 05/11/20 12:50 AM (3 years, 8 months ago)

Try using a syringe, and take a biopsy. Pc the syringe with some water in it and then take your biopsy and put a drop or two of water on the receiving plate. What type of contamination are you getting, bacteria, mold, yeast.


--------------------




Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 16 hours
Trusted Cultivator
Re: Agar infected again [Re: MH5109]
    #26663743 - 05/11/20 03:29 AM (3 years, 8 months ago)

You are flame sterilizing your scalpel, correct? That's what you mean by "disinfect"?

Just keep trying, you'll get it. Post some photos for us to help


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Offlinelittlemushroom
Stranger
Registered: 04/30/20
Posts: 32
Last seen: 3 years, 4 months
Re: Agar infected again [Re: mushpunx]
    #26665889 - 05/12/20 07:07 AM (3 years, 8 months ago)

Yes i always use flame to sterilize my blade.
The contaminations are yellow bacteria  and a bit white and transparet thing. I dont know these.  In the evening I try to take a good picture


Extras: Filter Print Post Top
InvisibleStoneTroegs
Mycopath
Registered: 11/04/18
Posts: 20
Re: Agar infected again [Re: littlemushroom]
    #26665898 - 05/12/20 07:14 AM (3 years, 8 months ago)

When you’re working in the SAB do you have any windows open or fans going? Your air may not be as still as it appears. I also use Ozium spray before any SAB work.
On that note. I’ve been getting a lot of what seems similar. Starts out white but fluffy. I usually think it’s just tomentose myc until it starts to take on a yellow/ orange tint. Almost what it looks like when you singe the end of your hair. Idk what it is. Only happens when I use SS that are older. I’m currently working on trying to transfer it out. Let’s see.


Extras: Filter Print Post Top
Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
Re: Agar infected again [Re: StoneTroegs]
    #26666009 - 05/12/20 08:22 AM (3 years, 8 months ago)

You don't wipe the scalpel after flame sterilization, right?


--------------------
How I do glass dishes


Extras: Filter Print Post Top
Invisiblegoatchild
mr noob


Registered: 10/31/19
Posts: 162
Re: Agar infected again [Re: littlemushroom]
    #26666080 - 05/12/20 09:01 AM (3 years, 8 months ago)

Are you using petri dishes? Do they come pre-sterilized? If so are you opening the plastic wrap inside the SAB?


--------------------
"What stands in the way becomes the way."


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Infected Syringe? Kalix 612 6 06/20/05 06:21 PM
by Kalix
* buying agar - help sinisha 3,416 6 03/31/06 05:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,382 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,156 8 02/25/05 11:03 AM
by agar
* test tubes agar fungophiliac 3,135 8 06/19/20 09:41 PM
by gorilliam
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 4,589 12 03/30/20 10:37 AM
by Surf707
* Agar and the global economy Dem_Bones 1,650 4 03/01/06 09:08 AM
by Dem_Bones
* Agar question WormholeSurfer 1,484 3 10/29/05 07:10 PM
by agar

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
166 topic views. 27 members, 170 guests and 28 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.01 seconds on 14 queries.