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Offlineouuwee
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Re: Pastywhyte's Easy Agar Tek [Re: SpaceDrooze]
    #26614070 - 04/19/20 11:13 PM (3 years, 9 months ago)

Has anyone ran into any issues during PCing with holes on the sides rather than the top of the lids? What should I be wary of?


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Offlinedroner66
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Nutrient defined and agar cosistency [Re: Pastywhyte]
    #26638916 - 04/30/20 12:19 PM (3 years, 9 months ago)

1. Can I assume that anything you add to your mix other than the agar is considered a nutrient? 

2. How do you determine if your agar is too soft or too hard?  Should it literally be soft and pliable to the touch like jello?


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Offlineoculodextro
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Contam issues [Re: Pastywhyte]
    #26639057 - 04/30/20 01:43 PM (3 years, 9 months ago)

I've done no-pour and poured plates and had great success, maybe 200 plates now.

Recently I can't shake contams. My lions mane always shows pink spots (P. aeruginosa?), and I've been getting slime mold everywhere.

Any ideas to fight these contams? I grab clean looking growth from early successful plates and still have contams arising.




This is every plate I do now. Doesn't matter if I'm prepouring or pouring in SAB. (PCing 40 mins, should I be filtering the agar?)


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Offlinejay.achS
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Re: Pastywhyte's Easy Agar Tek [Re: ouuwee]
    #26651131 - 05/05/20 04:06 PM (3 years, 9 months ago)

Quote:

ouuwee said:
Has anyone ran into any issues during PCing with holes on the sides rather than the top of the lids? What should I be wary of?




I have run 40 plates through with side holes without any issues.  I seem to do better when I only have one layer of micropore tape


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“One's destination is never a place, but a new way of seeing things.”  - Henry Miller

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Offlineouuwee
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Re: Pastywhyte's Easy Agar Tek [Re: jay.ach]
    #26651353 - 05/05/20 06:03 PM (3 years, 9 months ago)

Quote:

jay.ach said:
Quote:

ouuwee said:
Has anyone ran into any issues during PCing with holes on the sides rather than the top of the lids? What should I be wary of?




I have run 40 plates through with side holes without any issues.  I seem to do better when I only have one layer of micropore tape




What are the pros and cons of making holes on the sides? I have 50 mini rounds and I don't want to make holes and regret them later lol


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OfflineA.k.aM
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Re: Pastywhyte's Easy Agar Tek [Re: ouuwee]
    #26651366 - 05/05/20 06:09 PM (3 years, 9 months ago)

Oculo it sounds like it’s gotta be an issue with the pc unless something’s changed with your SAB or sterile technique or something similar.

I had a seal get slightly warped on a media bottle and had two rounds where every plate was covered in bacteria. Like the whole plate would be polka dotted 3-4 days later.


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Offlineoculodextro
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Re: Pastywhyte's Easy Agar Tek [Re: A.k.a]
    #26651478 - 05/05/20 07:01 PM (3 years, 9 months ago)

You're right I think my grain water just has to be PC's a ton longer. I just realized my test plates contammed out. Just threw out 50 plates I sterilized a day before.

I'm wondering If im stuffing the PC to the gills and not accounting for the extra mass, steam isn't penetrating.


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OfflineIshisoishi
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Re: Pastywhyte's Easy Agar Tek [Re: oculodextro]
    #26663029 - 05/10/20 07:04 PM (3 years, 9 months ago)

How much water should you put in the PC during the mini round sterilization process?


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Offlineoculodextro
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Re: Pastywhyte's Easy Agar Tek [Re: Ishisoishi]
    #26663240 - 05/10/20 08:38 PM (3 years, 9 months ago)

Quote:

A.k.a said:
Oculo it sounds like it’s gotta be an issue with the pc unless something’s changed with your SAB or sterile technique or something similar.

I had a seal get slightly warped on a media bottle and had two rounds where every plate was covered in bacteria. Like the whole plate would be polka dotted 3-4 days later.




Could switching agar brands lead to more contamination? Or maybe I should just get away from WBS agar. I'll be making some PDA and MEA to check if it's the grain water when I get some motivation.
Quote:



Ishisoishi said:
How much water should you put in the PC during the mini round sterilization process?



I tend to get the water level with the agar


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Offlinejay.achS
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Re: Pastywhyte's Easy Agar Tek [Re: ouuwee]
    #26663864 - 05/11/20 05:55 AM (3 years, 9 months ago)

Quote:

ouuwee said:

What are the pros and cons of making holes on the sides? I have 50 mini rounds and I don't want to make holes and regret them later lol



The pro is not having to use a paper towel and foil cover on each plate when you PC them.  The cons I know of are 1) the tape gets really wet  2) you have to be careful not to touch the tape.


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“One's destination is never a place, but a new way of seeing things.”  - Henry Miller

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OfflineA.k.aM
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Re: Pastywhyte's Easy Agar Tek [Re: jay.ach]
    #26664010 - 05/11/20 07:57 AM (3 years, 9 months ago)

Well that’s what I’m saying, if the pc is working right it shouldn’t matter what you’re using.

Try increasing pc time by like 30-50% and see what happens.


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Offlineouuwee
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Re: Pastywhyte's Easy Agar Tek [Re: A.k.a]
    #26664686 - 05/11/20 02:50 PM (3 years, 9 months ago)

If you put GE holes on the sides and the micropore tape gets wet during the cycle, will it get contaminated? Or it will only get contaminated when touched?


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Offlinerudimentary
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Re: Pastywhyte's Easy Agar Tek [Re: ouuwee]
    #26665088 - 05/11/20 06:16 PM (3 years, 9 months ago)

Does agar go bad? I've done this tek before but I can't get it to solidify this time for whatever reason. The potato flakes never really dissolve so what I end up with after they cool is basically red mashed potatoes.


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OfflinePsyche delics
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Re: Pastywhyte's Easy Agar Tek [Re: rudimentary]
    #26665141 - 05/11/20 06:44 PM (3 years, 9 months ago)

I've noticed the amount of agar in the op is really off and he uses different types of measurements. Confusing :confused:


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Offlinerudimentary
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Re: Pastywhyte's Easy Agar Tek [Re: Psyche delics] * 1
    #26665500 - 05/11/20 09:48 PM (3 years, 9 months ago)

Yeah I measured out the agar by weight and its definitely not 2 tablespoons worth. The agar itself dissolves but the potato flakes don't, are they supposed to stay mostly in tact? I've done it in the past with good results, kind of a Jello consistency or a little softer but I tried it today about 4 times and it never solidified, just grainy from the flakes. Not sure what I've been doing wrong. I didn't change the process at all.


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Offlineoculodextro
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Re: Pastywhyte's Easy Agar Tek [Re: rudimentary] * 1
    #26665557 - 05/11/20 10:32 PM (3 years, 9 months ago)

Quote:

rudimentary said:
Yeah I measured out the agar by weight and its definitely not 2 tablespoons worth. The agar itself dissolves but the potato flakes don't, are they supposed to stay mostly in tact? I've done it in the past with good results, kind of a Jello consistency or a little softer but I tried it today about 4 times and it never solidified, just grainy from the flakes. Not sure what I've been doing wrong. I didn't change the process at all.



Get a scale!

Also water temperature may play a big role in how well the starches dissolve. If I recall you want colder water.


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OfflinePsyche delics
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Re: Pastywhyte's Easy Agar Tek [Re: oculodextro]
    #26665585 - 05/11/20 10:46 PM (3 years, 9 months ago)


These are the truth for pasty plates


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Offlineoculodextro
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Re: Pastywhyte's Easy Agar Tek [Re: Psyche delics]
    #26665606 - 05/11/20 11:03 PM (3 years, 9 months ago)

I like hitting up goodwill and the likes, sometimes you can find a ton of these for dirt cheap!


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Offlinerudimentary
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Re: Pastywhyte's Easy Agar Tek [Re: oculodextro]
    #26665982 - 05/12/20 08:09 AM (3 years, 9 months ago)

I have a scale, I weighed the agar but not the potato flakes. When you say they dissolve better in colder water you mean start out with colder water and heat it up or there is no need to heat it up altogether? I know the agar dissolves regardless of temp but I haven't tried it with the potato flakes.


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Offlineoculodextro
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Re: Pastywhyte's Easy Agar Tek [Re: rudimentary]
    #26666035 - 05/12/20 08:39 AM (3 years, 9 months ago)

Been a minute since I worked with potato flakes. But if you look at OP he's bringing it all to boil on the stove then pouring into jars.

I would try to dissolve potato flakes in cold water, once it's mostly dissolved bring it slowly up to a boil and see if you get beter consistency.

I use potato flour so I know I need cold water for it to not get clumpy


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