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Offlineandmush
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Registered: 05/04/20
Posts: 16
Last seen: 3 years, 6 months
How is my mycelium guys?
    #26663137 - 05/10/20 07:57 PM (3 years, 8 months ago)

Its fluffy. First time getting this far in the process.  Its been about 2 weeks.  I just want to know if it looks contaminated or what.  I thought I heard somewhere it was not supposed to be this fluffy.  I've had a couple attempts where it was purple and I tossed them. No discoloration in this batch though, so that's good. I assumed bad spore syringe from the later attempts.



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OfflineJwardoin
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Registered: 02/09/15
Posts: 135
Last seen: 1 year, 11 months
Re: How is my mycelium guys? [Re: andmush]
    #26663191 - 05/10/20 08:24 PM (3 years, 8 months ago)

Did you go syringe > grains? Multispore genetics typically produce tomentose mycelium which can look a bit fuzzy/fluffy. Your jars look good so far but it would be impossible to tell if it's contaminated at this point. Keep your eye out for discoloration and see if they fully colonize. Take note of any spots that won't colonize. If everything colonizes, giving a good sniff will let you know if there's anything funky about it.

Also, if you did go MSS to grain, there's 100% change your syringe is not entirely clean. That's why everyone suggests going MSS > Agar > grain so you can without a doubt know that your culture is clean. That said, I hate agar, so my past grow I started MSS to grain.

Let these finish colonizing and try not to worry too much. Also remember to shake at 30% to speed things up!


Edited by Jwardoin (05/10/20 08:24 PM)


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Offlineandmush
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Registered: 05/04/20
Posts: 16
Last seen: 3 years, 6 months
Re: How is my mycelium guys? [Re: Jwardoin]
    #26663288 - 05/10/20 08:57 PM (3 years, 8 months ago)

I did just go syringe to grain also I didn't shake the jar when I inoculated so not a great job of spreading it out to begin with.  Thanks for the tips.


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