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Offlinerezasmith
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The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t
    #26662652 - 05/10/20 03:24 PM (3 years, 8 months ago)



title pretty much (last word is transfer)


Edited by rezasmith (05/10/20 03:25 PM)


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OfflineA.k.aM
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: rezasmith]
    #26662654 - 05/10/20 03:26 PM (3 years, 8 months ago)

Seems like there’s a good chance it’ll work.

Or you could pour a little layer of agar on top and wait for the myc to break through.


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Offlinerezasmith
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: A.k.a]
    #26662662 - 05/10/20 03:31 PM (3 years, 8 months ago)

Quote:

A.k.a said:
Seems like there’s a good chance it’ll work.

Or you could pour a little layer of agar on top and wait for the myc to break through.



so by pouring agar on top , since its fully colonized , it wouldnt get the bacteria in bottom? (cause mycelium pretty much colonized on bacteria)

and wouldn't i hurt the mycelium since its hot?
thanks for the advice


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OfflineA.k.aM
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: rezasmith]
    #26662678 - 05/10/20 03:40 PM (3 years, 8 months ago)

It’s called a hot pour.

I guess bacteria has trouble moving around so the myc can get away from it.


I’d let the agar cool til the last possible second before pouring it though.


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OfflineYeetusdeetus
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: rezasmith]
    #26662682 - 05/10/20 03:42 PM (3 years, 8 months ago)

Yeah search “warm pour” or “hot pour” using the search feature and click the box for trusted cultivators. There should be some threads detailing how the technique works.


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OfflineJwardoin
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: rezasmith]
    #26662686 - 05/10/20 03:44 PM (3 years, 8 months ago)

Quote:

so by pouring agar on top , since its fully colonized , it wouldnt get the bacteria in bottom? (cause mycelium pretty much colonized on bacteria)

and wouldn't i hurt the mycelium since its hot?
thanks for the advice





Essentially, bacteria and mold have no means of traveling through agar; they just chill on top. However, mycelium can eat through and travel to the top leaving the bacteria behind. It's called the sandwich tek. Hit that search bar to learn more.</font>


Edited by Jwardoin (05/10/20 03:45 PM)


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OfflineMycoactive
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: Jwardoin]
    #26662691 - 05/10/20 03:47 PM (3 years, 8 months ago)

I would just take a transfer from the climbing edge. Seems like the easiest solution.


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OfflineYeetusdeetus
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: Jwardoin]
    #26662821 - 05/10/20 05:15 PM (3 years, 8 months ago)

Quote:

Jwardoin said:
Quote:

so by pouring agar on top , since its fully colonized , it wouldnt get the bacteria in bottom? (cause mycelium pretty much colonized on bacteria)

and wouldn't i hurt the mycelium since its hot?
thanks for the advice





Essentially, bacteria and mold have no means of traveling through agar; they just chill on top. However, mycelium can eat through and travel to the top leaving the bacteria behind. It's called the sandwich tek. Hit that search bar to learn more.</font>




Mold can go through but not bacteria I’m pretty sure lol


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Offlinemushpunx
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: rezasmith]
    #26662944 - 05/10/20 06:34 PM (3 years, 8 months ago)

Quote:

rezasmith said:


title pretty much (last word is transfer)





Is this a germination plate?


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Invisiblegizmo1
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: A.k.a] * 1
    #26663141 - 05/10/20 07:58 PM (3 years, 8 months ago)

Quote:

A.k.a said:
It’s called a hot pour.

I guess bacteria has trouble moving around so the myc can get away from it.


I’d let the agar cool til the last possible second before pouring it though.



I like to pour pretty damn hot I let it cool some but not like I would if pouring plates.
I keep glass no pours around. When I need to hot pour I'll just heat one until it's boiling let it cool a couple mins until I can handle it and pour it over bacterial plate. You want to grab the mycelium as soon as it pokes through the top. Also when you transfer don't take a wedge scrape the myc off the top and transfer to another plate.


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Offlinemushpunx
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: gizmo1]
    #26663184 - 05/10/20 08:21 PM (3 years, 8 months ago)

Quote:

gizmo1 said:
Quote:

A.k.a said:
It’s called a hot pour.

I guess bacteria has trouble moving around so the myc can get away from it.


I’d let the agar cool til the last possible second before pouring it though.



I like to pour pretty damn hot I let it cool some but not like I would if pouring plates.
I keep glass no pours around. When I need to hot pour I'll just heat one until it's boiling let it cool a couple mins until I can handle it and pour it over bacterial plate. You want to grab the mycelium as soon as it pokes through the top. Also when you transfer don't take a wedge scrape the myc off the top and transfer to another plate.




I have never had to do this before,but that is pretty cool actually.


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Offlinerezasmith
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: mushpunx]
    #26663782 - 05/11/20 04:31 AM (3 years, 8 months ago)

Quote:

mushpunx said:
Quote:

rezasmith said:


title pretty much (last word is transfer)





Is this a germination plate?




its agar to agar , i'll probably trash it if my other transfers succeed (waiting for them) but this was something that i was wondering and i got some useful information out of it


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Offlinepoisoned
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: rezasmith]
    #26663790 - 05/11/20 04:35 AM (3 years, 8 months ago)

It's worth it to try the method out before you actually need it. I was rescuing a culture from a contaminated slant this year. I succeeded, but I'd appreciate if I had the skill before it was actually needed.


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Offlinerezasmith
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: gizmo1]
    #26663806 - 05/11/20 04:51 AM (3 years, 8 months ago)

Quote:

gizmo1 said:
Quote:

A.k.a said:
It’s called a hot pour.

I guess bacteria has trouble moving around so the myc can get away from it.


I’d let the agar cool til the last possible second before pouring it though.



I like to pour pretty damn hot I let it cool some but not like I would if pouring plates.
I keep glass no pours around. When I need to hot pour I'll just heat one until it's boiling let it cool a couple mins until I can handle it and pour it over bacterial plate. You want to grab the mycelium as soon as it pokes through the top. Also when you transfer don't take a wedge scrape the myc off the top and transfer to another plate.





I tried to only get mycelium not agar before on different plates but that only lead to failure for me , the mycelium is so god damn sticky when it sticks to your blade you cant shake it off to dish at all , atleast i couldn't
any advice on how you do it that it doesn't stick?


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Offlinepoisoned
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: rezasmith]
    #26663827 - 05/11/20 05:12 AM (3 years, 8 months ago)

You should be doing that transfer before it even gets thick. As soon as you see a few strands poking through, get your inoculation loop and do the transfer.


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Offlinerezasmith
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: poisoned]
    #26663836 - 05/11/20 05:19 AM (3 years, 8 months ago)

Quote:

poisoned said:
You should be doing that transfer before it even gets thick. As soon as you see a few strands poking through, get your inoculation loop and do the transfer.




well shit i don't have one , is inoculation loop better for all transfers? i've been having a tough time sometimes trying to transfer with blade so that's why im asking


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Offlinepoisoned
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: rezasmith] * 1
    #26663843 - 05/11/20 05:26 AM (3 years, 8 months ago)

I only use inoculation loop when I'm inoculating spores or when I'm trying to do those invisibly small amount of culture transfers. Scalpel is better for most transfers.

Inoculation loop can be quickly and easily DIYed at home. It's just a piece of wire. Put it around a chopstick or a small screwdriver and twist it up and you have a noc loop.

When doing transfers with a scalpel, you should try dipping it into receiving plate backwards (cutting edge pointing upwards). Myc seems to get off that way easier.


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Offlinemushpunx
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Re: The plate is bacterial . But do you think its worth/possible to take the edge that is climbing for t [Re: rezasmith]
    #26663876 - 05/11/20 06:19 AM (3 years, 8 months ago)

Quote:

rezasmith said:
Quote:

mushpunx said:
Quote:

rezasmith said:


title pretty much (last word is transfer)





Is this a germination plate?




its agar to agar , i'll probably trash it if my other transfers succeed (waiting for them) but this was something that i was wondering and i got some useful information out of it





Ah okay so this was a donor plate you took transfers from, yeah?
I put mine in cold storage once colonization reaches 2/3 of the plate or so. It gives you a little room to watch for contams, also in pitre dishes it's a risk if the myc crawls up the sides. Not so much in deep containers.

I use a scalpel for my transfers, and a loop for inoculation from prints and swiping spores. A thin piece of wire, nichrome if you can get it works well, you twist a little loop around something round and you can hold the ends in an exactly knife handle.
Actual loops are real cheap though, just grab one when you do your next order from a mycology page.

https://fungi.com/products/full-spear-scalpel

This is the scalpel I use and I love it. The way the blade is shaped , it's not so thin that the wedges stick to it. Easy to clean too, holds up to flame.


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