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OfflineWendetaWasp
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I want perfect lids! Lots of questions.
    #26662209 - 05/10/20 11:43 AM (3 years, 9 months ago)

My goal is to create a lid that would be reusable at least 10 times. I have problem with 2 things.
First is high-temp silicone injection sites: I never managed to make it self-sealing and the silicone always got inside the needle. It looks like I should use a smaller needle, but I think smaller size would not be enough for spores (I use the 1.2 mm). In the end I replaced it with real medical injection sites and I'm satisfied with that, but still I would like to know, why I had such problems.
Second would be some breathable material. I've read many times that tyvek is one use only, but I don't understand why, and replacing it every time is such a hassle.
Mason jars are not the norm in my country, I use european-type twisting lids with two drilled holes, one for injection and one for breathable material. At this time I'm using masking tape, so of course I need to replace them every time. It looks very close to damage after sterilization, so I have doubts on it's efficiency.
So should I go for MP tape and stop inventing, or is there any great reusable material? Is MP tape one use only? Is tyvek really one-use only? (not postal tyvek, I have access only to tyvek soft atireflex). Have you tried Guttafol instead of tyvek?
Last question might be a little bit stupid, but is wrapping tinfoil over lids with tyvek really necessary? Stop buying tinfoil would be really great too.
You see, I'm a bit of a no-waste freak :P

Thanks for answers


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OfflineLogicaL ChaosM
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Re: I want perfect lids! Lots of questions. [Re: WendetaWasp]
    #26662215 - 05/10/20 11:46 AM (3 years, 9 months ago)

Id say micropore tape is the best way. And its cheap too. Make sure its 3M brand.

The tin foil over the jar lids to prevent water from dripping into the jars during the PC cycle. Very much needed but u can reuse them lots of times.

For FAE lids, try Polyfelt. They are the best.


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Offlinepoisoned
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] * 1
    #26662255 - 05/10/20 12:05 PM (3 years, 9 months ago)

I tried out MP tape a few weeks ago and lost all jars in a matter of a week. Maybe I should've double layered or sth, but I'm not sure.

Tyvek should be multi use AFAIK.

Tin foil is necessary, but you don't need new one every time. Just reuse.


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OfflineLogicaL ChaosM
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Re: I want perfect lids! Lots of questions. [Re: poisoned]
    #26662259 - 05/10/20 12:07 PM (3 years, 9 months ago)

Dude! Definitely layer them next time or use smaller holes in the lid :yesnod:

Polylids are legit. So is Tyvek.


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Offlinetripdawg420
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Re: I want perfect lids! Lots of questions. [Re: poisoned] * 1
    #26662261 - 05/10/20 12:08 PM (3 years, 9 months ago)

sfd :thumbup:


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Offlinepoisoned
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] * 1
    #26662267 - 05/10/20 12:10 PM (3 years, 9 months ago)

How many layers are you using? I might try a new batch in a few days.


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OfflineLogicaL ChaosM
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Re: I want perfect lids! Lots of questions. [Re: poisoned]
    #26662276 - 05/10/20 12:13 PM (3 years, 9 months ago)

Layers of what?


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Offlinepoisoned
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] * 1
    #26662286 - 05/10/20 12:16 PM (3 years, 9 months ago)

Layers of MP


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OfflineLogicaL ChaosM
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Re: I want perfect lids! Lots of questions. [Re: poisoned] * 1
    #26662288 - 05/10/20 12:17 PM (3 years, 9 months ago)

Oh. I use two to three or one layer of medical cloth tape. Both seem to work pretty good.


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Offlineoculodextro
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] * 1
    #26662315 - 05/10/20 12:28 PM (3 years, 9 months ago)

I tend to put my MP tape in different directions. Should technically  make the pores smaller.

Do a piece vertically and a piece horizontally. I'll have to look under my scope to make sure I'm not BSing here.


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OfflineSockadin
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Re: I want perfect lids! Lots of questions. [Re: WendetaWasp] * 1
    #26662325 - 05/10/20 12:32 PM (3 years, 9 months ago)

You should try not shooting spores in to grain jars. :shrug: maybe PF Tek?


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OfflineLogicaL ChaosM
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Re: I want perfect lids! Lots of questions. [Re: oculodextro]
    #26662334 - 05/10/20 12:37 PM (3 years, 9 months ago)

Same. Go random pattern.

Missed the spore syringe to jar comment. Yeah, dont do that. Go spores to agar Then to grain jars/bags.


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InvisiblebodhisattaMDiscordReddit
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] * 1
    #26662377 - 05/10/20 12:50 PM (3 years, 9 months ago)

Only good lid with SFDs or polyfil.


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OfflineLogicaL ChaosM
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Re: I want perfect lids! Lots of questions. [Re: bodhisatta]
    #26662381 - 05/10/20 12:51 PM (3 years, 9 months ago)

Most def.

I used to use polyfill but its a bit of a pain in the ass to use.


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OfflineSockadin
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Re: I want perfect lids! Lots of questions. [Re: bodhisatta]
    #26662503 - 05/10/20 01:44 PM (3 years, 9 months ago)

I have been using Micropore tape cause (I'm lazy) and I have to say that I have tossed more damn jars this week than I have in a long time. I'm going to go back to SFD's or Watmans.

That being said I'm usually 6-8 beers in before I ever have time to start doing wedge transfers so it probably me and not the tape.


Edit: WHAT THE HELL HAPPENED!  Syringe Filters on Amazon went up 2000%. Use to be able to get a pack of 25 for like 20 bucks. Now they are selling 50 for 200-350?


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InvisibleRoger Clemency
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Re: I want perfect lids! Lots of questions. [Re: Sockadin] * 1
    #26662515 - 05/10/20 01:51 PM (3 years, 9 months ago)

You should check out synthetic filter disks (SFD)

You can Get 7 quarter sized circles (or a similar sized coin of your country) from a single disk that costs about $1 US. And they last a long time.

I made some jars with injection ports when I first started but I didn’t trust them. I’m not sure if you’re shooting spore syringes to grain which is risky, but you really don’t need injection ports even if you want to use LC or spores or any other kind of liquid. Just crack the lid in a SAB and squirt in.


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OfflineLogicaL ChaosM
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Re: I want perfect lids! Lots of questions. [Re: Sockadin]
    #26662516 - 05/10/20 01:51 PM (3 years, 9 months ago)

what the hell! Damn you Supply and Demand scalping!!! :crankey:


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OfflineSockadin
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Re: I want perfect lids! Lots of questions. [Re: Roger Clemency] * 1
    #26662539 - 05/10/20 02:03 PM (3 years, 9 months ago)

Even true SFD's on Amazon are up to 1.85

Be careful there are alot of cellulose disks people are pushing as SFD's..
I guess it's back to poly or maybe I should just change out my Micropore tape between uses haha.


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Invisiblegizmo1
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Re: I want perfect lids! Lots of questions. [Re: Sockadin]
    #26662603 - 05/10/20 02:48 PM (3 years, 9 months ago)

Quote:

Sockadin said:
Even true SFD's on Amazon are up to 1.85

Be careful there are alot of cellulose disks people are pushing as SFD's..
I guess it's back to poly or maybe I should just change out my Micropore tape between uses haha.



The cellulose kinda suck but they work. I've got like 10 I've been using for the last 3 or 4 years. I use them whole and don't wash them between uses. Oyster like to colonize the shit out of them but even then I reuse them lmao.
That sucks that they are raising prices on use I just bought a 10 pack of syringe filters like a month ago for around 10 bucks haven't looked since but I'm glad I got them when I did.


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Invisiblegizmo1
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Re: I want perfect lids! Lots of questions. [Re: Sockadin]
    #26662610 - 05/10/20 02:55 PM (3 years, 9 months ago)

Quote:

Sockadin said:
I have been using Micropore tape cause (I'm lazy) and I have to say that I have tossed more damn jars this week than I have in a long time. I'm going to go back to SFD's or Watmans.

That being said I'm usually 6-8 beers in before I ever have time to start doing wedge transfers so it probably me and not the tape.


Edit: WHAT THE HELL HAPPENED!  Syringe Filters on Amazon went up 2000%. Use to be able to get a pack of 25 for like 20 bucks. Now they are selling 50 for 200-350?



https://www.amazon.com/Syringe-Filters-Diameter-0-22um-Sterile/dp/B06XWTDPR6/ref=mp_s_a_1_2_sspa?dchild=1&keywords=syringe+filter&qid=1589143919&sr=8-2-spons&psc=1&spLa=ZW5jcnlwdGVkUXVhbGlmaWVyPUEzTVlaWTgwV1NRUkRGJmVuY3J5cHRlZElkPUEwOTI3MjE5M00xRE9XUUNJV0VGViZlbmNyeXB0ZWRBZElkPUEwODQ4MjU3MUoyMjhPSTFLNjE1UCZ3aWRnZXROYW1lPXNwX3Bob25lX3NlYXJjaF9hdGYmYWN0aW9uPWNsaWNrUmVkaXJlY3QmZG9Ob3RMb2dDbGljaz10cnVl
All the ones I found are priced about right they aren't whatmans but they will work.


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