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WendetaWasp
Lab rat



Registered: 03/31/20
Posts: 6
Loc: Europe
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I want perfect lids! Lots of questions.
#26662209 - 05/10/20 11:43 AM (3 years, 8 months ago) |
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My goal is to create a lid that would be reusable at least 10 times. I have problem with 2 things. First is high-temp silicone injection sites: I never managed to make it self-sealing and the silicone always got inside the needle. It looks like I should use a smaller needle, but I think smaller size would not be enough for spores (I use the 1.2 mm). In the end I replaced it with real medical injection sites and I'm satisfied with that, but still I would like to know, why I had such problems. Second would be some breathable material. I've read many times that tyvek is one use only, but I don't understand why, and replacing it every time is such a hassle. Mason jars are not the norm in my country, I use european-type twisting lids with two drilled holes, one for injection and one for breathable material. At this time I'm using masking tape, so of course I need to replace them every time. It looks very close to damage after sterilization, so I have doubts on it's efficiency. So should I go for MP tape and stop inventing, or is there any great reusable material? Is MP tape one use only? Is tyvek really one-use only? (not postal tyvek, I have access only to tyvek soft atireflex). Have you tried Guttafol instead of tyvek? Last question might be a little bit stupid, but is wrapping tinfoil over lids with tyvek really necessary? Stop buying tinfoil would be really great too. You see, I'm a bit of a no-waste freak :P
Thanks for answers
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
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Re: I want perfect lids! Lots of questions. [Re: WendetaWasp]
#26662215 - 05/10/20 11:46 AM (3 years, 8 months ago) |
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Id say micropore tape is the best way. And its cheap too. Make sure its 3M brand.
The tin foil over the jar lids to prevent water from dripping into the jars during the PC cycle. Very much needed but u can reuse them lots of times.
For FAE lids, try Polyfelt. They are the best.
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏
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poisoned
untitled



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Posts: 1,738
Loc: Yurop
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] 1
#26662255 - 05/10/20 12:05 PM (3 years, 8 months ago) |
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I tried out MP tape a few weeks ago and lost all jars in a matter of a week. Maybe I should've double layered or sth, but I'm not sure.
Tyvek should be multi use AFAIK.
Tin foil is necessary, but you don't need new one every time. Just reuse.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible...
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Re: I want perfect lids! Lots of questions. [Re: poisoned]
#26662259 - 05/10/20 12:07 PM (3 years, 8 months ago) |
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Dude! Definitely layer them next time or use smaller holes in the lid 
Polylids are legit. So is Tyvek.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
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Re: I want perfect lids! Lots of questions. [Re: poisoned] 1
#26662261 - 05/10/20 12:08 PM (3 years, 8 months ago) |
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sfd
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] 1
#26662267 - 05/10/20 12:10 PM (3 years, 8 months ago) |
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How many layers are you using? I might try a new batch in a few days.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
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Re: I want perfect lids! Lots of questions. [Re: poisoned]
#26662276 - 05/10/20 12:13 PM (3 years, 8 months ago) |
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Layers of what?
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] 1
#26662286 - 05/10/20 12:16 PM (3 years, 8 months ago) |
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Layers of MP
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
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Re: I want perfect lids! Lots of questions. [Re: poisoned] 1
#26662288 - 05/10/20 12:17 PM (3 years, 8 months ago) |
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Oh. I use two to three or one layer of medical cloth tape. Both seem to work pretty good.
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oculodextro

Registered: 04/10/13
Posts: 1,205
Loc: Space is the Place
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] 1
#26662315 - 05/10/20 12:28 PM (3 years, 8 months ago) |
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I tend to put my MP tape in different directions. Should technically make the pores smaller.
Do a piece vertically and a piece horizontally. I'll have to look under my scope to make sure I'm not BSing here.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: I want perfect lids! Lots of questions. [Re: WendetaWasp] 1
#26662325 - 05/10/20 12:32 PM (3 years, 8 months ago) |
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You should try not shooting spores in to grain jars. maybe PF Tek?
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
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Re: I want perfect lids! Lots of questions. [Re: oculodextro]
#26662334 - 05/10/20 12:37 PM (3 years, 8 months ago) |
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Same. Go random pattern.
Missed the spore syringe to jar comment. Yeah, dont do that. Go spores to agar Then to grain jars/bags.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] 1
#26662377 - 05/10/20 12:50 PM (3 years, 8 months ago) |
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Only good lid with SFDs or polyfil.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
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Re: I want perfect lids! Lots of questions. [Re: bodhisatta]
#26662381 - 05/10/20 12:51 PM (3 years, 8 months ago) |
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Most def.
I used to use polyfill but its a bit of a pain in the ass to use.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: I want perfect lids! Lots of questions. [Re: bodhisatta]
#26662503 - 05/10/20 01:44 PM (3 years, 8 months ago) |
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I have been using Micropore tape cause (I'm lazy) and I have to say that I have tossed more damn jars this week than I have in a long time. I'm going to go back to SFD's or Watmans.
That being said I'm usually 6-8 beers in before I ever have time to start doing wedge transfers so it probably me and not the tape.
Edit: WHAT THE HELL HAPPENED! Syringe Filters on Amazon went up 2000%. Use to be able to get a pack of 25 for like 20 bucks. Now they are selling 50 for 200-350?
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Re: I want perfect lids! Lots of questions. [Re: Sockadin] 1
#26662515 - 05/10/20 01:51 PM (3 years, 8 months ago) |
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You should check out synthetic filter disks (SFD)
You can Get 7 quarter sized circles (or a similar sized coin of your country) from a single disk that costs about $1 US. And they last a long time.
I made some jars with injection ports when I first started but I didn’t trust them. I’m not sure if you’re shooting spore syringes to grain which is risky, but you really don’t need injection ports even if you want to use LC or spores or any other kind of liquid. Just crack the lid in a SAB and squirt in.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible...
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Re: I want perfect lids! Lots of questions. [Re: Sockadin]
#26662516 - 05/10/20 01:51 PM (3 years, 8 months ago) |
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what the hell! Damn you Supply and Demand scalping!!!
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Sockadin



Registered: 01/03/10
Posts: 7,244
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Re: I want perfect lids! Lots of questions. [Re: Roger Clemency] 1
#26662539 - 05/10/20 02:03 PM (3 years, 8 months ago) |
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Even true SFD's on Amazon are up to 1.85
Be careful there are alot of cellulose disks people are pushing as SFD's.. I guess it's back to poly or maybe I should just change out my Micropore tape between uses haha.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: I want perfect lids! Lots of questions. [Re: Sockadin]
#26662603 - 05/10/20 02:48 PM (3 years, 8 months ago) |
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Quote:
Sockadin said: Even true SFD's on Amazon are up to 1.85
Be careful there are alot of cellulose disks people are pushing as SFD's.. I guess it's back to poly or maybe I should just change out my Micropore tape between uses haha.
The cellulose kinda suck but they work. I've got like 10 I've been using for the last 3 or 4 years. I use them whole and don't wash them between uses. Oyster like to colonize the shit out of them but even then I reuse them lmao. That sucks that they are raising prices on use I just bought a 10 pack of syringe filters like a month ago for around 10 bucks haven't looked since but I'm glad I got them when I did.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: I want perfect lids! Lots of questions. [Re: Sockadin]
#26662610 - 05/10/20 02:55 PM (3 years, 8 months ago) |
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Quote:
Sockadin said: I have been using Micropore tape cause (I'm lazy) and I have to say that I have tossed more damn jars this week than I have in a long time. I'm going to go back to SFD's or Watmans.
That being said I'm usually 6-8 beers in before I ever have time to start doing wedge transfers so it probably me and not the tape.
Edit: WHAT THE HELL HAPPENED! Syringe Filters on Amazon went up 2000%. Use to be able to get a pack of 25 for like 20 bucks. Now they are selling 50 for 200-350?
https://www.amazon.com/Syringe-Filters-Diameter-0-22um-Sterile/dp/B06XWTDPR6/ref=mp_s_a_1_2_sspa?dchild=1&keywords=syringe+filter&qid=1589143919&sr=8-2-spons&psc=1&spLa=ZW5jcnlwdGVkUXVhbGlmaWVyPUEzTVlaWTgwV1NRUkRGJmVuY3J5cHRlZElkPUEwOTI3MjE5M00xRE9XUUNJV0VGViZlbmNyeXB0ZWRBZElkPUEwODQ4MjU3MUoyMjhPSTFLNjE1UCZ3aWRnZXROYW1lPXNwX3Bob25lX3NlYXJjaF9hdGYmYWN0aW9uPWNsaWNrUmVkaXJlY3QmZG9Ob3RMb2dDbGljaz10cnVl All the ones I found are priced about right they aren't whatmans but they will work.
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Justweed
Bobby's short shorts



Registered: 04/01/20
Posts: 555
Last seen: 3 months, 17 days
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Re: I want perfect lids! Lots of questions. [Re: Sockadin] 1
#26662611 - 05/10/20 02:56 PM (3 years, 8 months ago) |
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I do my jars with micropore tape, I make an X with tape over the hole (1 hole, 3/8 inch punched with a leather puncher, works great), PC with foil over the lid as usual, then when noccing jars (g2g, spores on grain, or LC syringe) I tap the tape with alcohol on a paper towel, then use another paper towel to pat dry, then put another piece of tape going between the X<(Here) so there' kind of 3 layers all going different directions. Only time I've been getting contams is from using contamed starting material, definitely no issues with MP on the holes.
Has anyone found a reusable lid cover that stays reusable, like a plastic cover of some sort? Is there any 3D Printable plastic that can be PC'd?
--------------------
  Keep 'em high and tight guys....
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oculodextro

Registered: 04/10/13
Posts: 1,205
Loc: Space is the Place
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Re: I want perfect lids! Lots of questions. [Re: Justweed] 1
#26662655 - 05/10/20 03:27 PM (3 years, 8 months ago) |
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Quote:
Justweed said:
Has anyone found a reusable lid cover that stays reusable, like a plastic cover of some sort? Is there any 3D Printable plastic that can be PC'd?
What about that modeling clay (Sculptee?) molded to the top, just brainstorming here.
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Re: I want perfect lids! Lots of questions. [Re: oculodextro] 1
#26662683 - 05/10/20 03:42 PM (3 years, 8 months ago) |
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What kind of lid cover are you talking about? Like a cover for the PC run?
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: I want perfect lids! Lots of questions. [Re: Roger Clemency]
#26662731 - 05/10/20 04:16 PM (3 years, 8 months ago) |
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Best lid design I've ever used. Highly recommend doing them like this https://www.shroomery.org/forums/showflat.php/Number/26498354/page/3
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
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Re: I want perfect lids! Lots of questions. [Re: WendetaWasp] 1
#26662739 - 05/10/20 04:21 PM (3 years, 8 months ago) |
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OP - what are you using these jars for, grain spawn?
1. The injection port is completely unnecessary. You should be doing all inoculations in a still air box. Open the lid to inoculate.
2. If you need the injection port because you are inoculating grains with a spore syringe , stop. You should really only inoculate grains with a clean culture on agar. If the injection port is for injecting liquid culture in, just unscrew the lid and pour or squirt the LC in.
3. The best lids for grain spawn are Synthetic Filter Discs or polyfill. Since you aren't using Mason jars, I assume you have the one piece metal or plastic lids. These are perfect for polyfill. Just drill your GE hole and stuff tightly with polyfill.
4. Yes, you need to cover the lids with foil during your PC run.
This is what I use - I get those autoclavable plastic lids for spawn jars from mycology suppliers, they are dirt cheap and never warp. I think they are better than the plastic Mason jar lids, which are really only meant for storage. I drill a hole in the center and pop the SFD right up under the lid. I also will use polyfill. These plastic lids are perfect for that, the 2 piece Mason jar metal band and lid are a pain to hold together.
Here is an example (this is actually a gallon spawn jar, that's why it has 3 holes instead of one. Drilling can crack the lids, so I prefer to heat up the back of a drill bit with a propane torch and punch a hole thru
--------------------
 Amateur Mycologists United AMU Q&A
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Re: I want perfect lids! Lots of questions. [Re: oculodextro]
#26662755 - 05/10/20 04:30 PM (3 years, 8 months ago) |
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Just had an idea. Bring back the syringe lock-on-lid idea, install into the lid and use only LC with a syringe. That way you have a interface that doesnt wear out like other syringe-poking ideas.
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Domno
Magician



Registered: 01/30/20
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Re: I want perfect lids! Lots of questions. [Re: natedawgnow] 1
#26662784 - 05/10/20 04:50 PM (3 years, 8 months ago) |
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Just drill a hole in the middle of the lid. Stuff with pilyfill or mp tape. Then you can inject through the polypill or tape. With the tape make sure to put another piece over the top after injecting. Look into agar. Little extra time much better chances.
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos] 1
#26662787 - 05/10/20 04:51 PM (3 years, 8 months ago) |
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For filters I cut squares using a scalpel out of this roll of "beading felt" I got at the Fabric store. Haven't lost a jar yet.
It's basically polyfil that's been flattened by heat. Stiffer than fuck and a square meter of it cost me $11 so I'll probably never run out.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: I want perfect lids! Lots of questions. [Re: LogicaL Chaos]
#26662905 - 05/10/20 06:13 PM (3 years, 8 months ago) |
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Quote:
LogicaL Chaos said: Just had an idea. Bring back the syringe lock-on-lid idea, install into the lid and use only LC with a syringe. That way you have a interface that doesnt wear out like other syringe-poking ideas.
Why not just open the lid?
--------------------
 Amateur Mycologists United AMU Q&A
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
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Re: I want perfect lids! Lots of questions. [Re: starbones]
#26662909 - 05/10/20 06:16 PM (3 years, 8 months ago) |
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Quote:
starbones said: For filters I cut squares using a scalpel out of this roll of "beading felt" I got at the Fabric store. Haven't lost a jar yet.
It's basically polyfil that's been flattened by heat. Stiffer than fuck and a square meter of it cost me $11 so I'll probably never run out.
I used to cut out circles of white Kraft felt (basically the same stuff), which I would pinch between the metal lid and band, over the GE hole. It is nice because it holds the lid and band together.
But these days I've found it's better to go with plastic lid with polyfill
--------------------
 Amateur Mycologists United AMU Q&A
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Justweed
Bobby's short shorts



Registered: 04/01/20
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Re: I want perfect lids! Lots of questions. [Re: Roger Clemency]
#26665020 - 05/11/20 05:32 PM (3 years, 8 months ago) |
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Quote:
oculodextro said: What about that modeling clay (Sculptee?) molded to the top, just brainstorming here.
Not sure if clay can be PC'd at high temp+moisture.
Quote:
Roger Clemency said: What kind of lid cover are you talking about? Like a cover for the PC run?
Yea, instead of aluminum foil that gets tossed after a few runs (or if you're wasteful like me recycled every run).
--------------------
  Keep 'em high and tight guys....
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Re: I want perfect lids! Lots of questions. [Re: Justweed]
#26665064 - 05/11/20 06:00 PM (3 years, 8 months ago) |
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Ah! Every run is indeed a lot o foil. I will use them basically forever but I definitely wouldn’t mind a cheap, but quality slip on type cover.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
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Re: I want perfect lids! Lots of questions. [Re: bodhisatta]
#26665100 - 05/11/20 06:22 PM (3 years, 8 months ago) |
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Quote:
tripdawg420 said: sfd 
Quote:
bodhisatta said: Only good lid with SFDs or polyfil.
SFDs...perfection. Nothing beats a micron filter for your jar.
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woofwoof
such mushrooms!



Registered: 01/04/19
Posts: 1,127
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Re: I want perfect lids! Lots of questions. [Re: rumfor69]
#26665656 - 05/12/20 12:17 AM (3 years, 8 months ago) |
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I use good old polyfil. When I first started growing, I would just shoot grain jar up by poking flame steriilzed needle thru the polyfil and squirt 2 ccs to each jar.
Now I do agar and LI only. Just lift the lid and pour LI in, easy and have no contams as of yet.
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chuckarama


Registered: 03/21/20
Posts: 79
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Re: I want perfect lids! Lots of questions. [Re: woofwoof]
#26665763 - 05/12/20 04:28 AM (3 years, 8 months ago) |
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+1 on the craft felt I'm still making use of the first 30x30cm sheet I bought, and the first set of lids I made in 2016. Reusable with or without the injection port.
Easy Felt Tek Self Healing Lid
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B21
PeaceLoveShrooms



Registered: 04/09/20
Posts: 871
Loc: Derby City
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Re: I want perfect lids! Lots of questions. [Re: chuckarama]
#26665782 - 05/12/20 04:49 AM (3 years, 8 months ago) |
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Idk if this is allowed but this place has these awesome adhesive sfd’s that work beautifully https://highdesertspores.com/products/self-adhesive-breathable-0-22-micron-filter-patch As far as syringe inoculation ports....I inoculate with agar wedges or g2g so my lids only have one hole for ge. ?♂️
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: I want perfect lids! Lots of questions. [Re: B21]
#26666233 - 05/12/20 10:28 AM (3 years, 8 months ago) |
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Quote:
B21 said: Idk if this is allowed but this place has these awesome adhesive sfd’s that work beautifully https://highdesertspores.com/products/self-adhesive-breathable-0-22-micron-filter-patch As far as syringe inoculation ports....I inoculate with agar wedges or g2g so my lids only have one hole for ge. ?♂️
Those are pretty damn cool. They look a little thin are they? Also how well does the adhesive hold up does it shift while hot?
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B21
PeaceLoveShrooms



Registered: 04/09/20
Posts: 871
Loc: Derby City
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Re: I want perfect lids! Lots of questions. [Re: gizmo1]
#26682622 - 05/20/20 06:07 AM (3 years, 8 months ago) |
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Sorry for the late response but they are kinda thin but I’ve reused the lids I made with them 3 times so far. I’d say they work better than mp tape but I’ve never used mp tape as a filtered lid. I’m a huge tyvek fan too. I’d never go through the trouble of stuffing polyfil in my lids lol. But these things work well and if someone is “lazy” I’d say it’s their best route. GL **they don’t shift while hot ime
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: I want perfect lids! Lots of questions. [Re: B21]
#26685586 - 05/21/20 12:09 PM (3 years, 8 months ago) |
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Quote:
B21 said: Sorry for the late response but they are kinda thin but I’ve reused the lids I made with them 3 times so far. I’d say they work better than mp tape but I’ve never used mp tape as a filtered lid. I’m a huge tyvek fan too. I’d never go through the trouble of stuffing polyfil in my lids lol. But these things work well and if someone is “lazy” I’d say it’s their best route. GL **they don’t shift while hot ime
It's all good I may have to give them a try I'd never heard of them before you posted the link.
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