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OfflineMuckPeddler
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Keep getting bacteria?? * 1
    #26660269 - 05/09/20 03:32 PM (3 years, 8 months ago)

Hey y'all, so its now like my 3rd attempt at PF tek and for some reason I keep getting yellowy bacteria? The first time I didnt know that I shouldn't wipe my needle off to remove the char so my mistake, I am guessing that messed it up. Second time I am not sure what happened maybe my vermiculite+BRF was too wet. But this last time I steam sterilized for 95 minutes, made sure that my verm was at field capacity and everything and bacteria is still poppin up.I use a SAB and wear gloves idk I try to keep things clean as possible. I know bacteria is common I am just confused lol. Any idea of what could be happening?


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InvisibleRiverBum
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Re: Keep getting bacteria?? [Re: MuckPeddler] * 1
    #26660276 - 05/09/20 03:36 PM (3 years, 8 months ago)

Pictures would help, it could just be metabolites. Are you flame sterilizing the syringe between jars?


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OfflineMuckPeddler
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Re: Keep getting bacteria?? [Re: RiverBum]
    #26660286 - 05/09/20 03:42 PM (3 years, 8 months ago)

Yes I have been flame sterilizing between jars! Ill grab some pictures of them later but basically its like a yellow goopy slime


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InvisibleRiverBum
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Re: Keep getting bacteria?? [Re: MuckPeddler]
    #26660305 - 05/09/20 03:54 PM (3 years, 8 months ago)

How much spore solution are you injecting into each jar? If you are using too much that can lead to problems


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OfflineMuckPeddler
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Re: Keep getting bacteria?? [Re: RiverBum]
    #26660331 - 05/09/20 04:08 PM (3 years, 8 months ago)

This last one I used around 3mL for each jar, is that too much?


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OfflineLogicaL ChaosM
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Re: Keep getting bacteria?? [Re: RiverBum]
    #26660335 - 05/09/20 04:09 PM (3 years, 8 months ago)

Quote:

RiverBum said:
Pictures would help, it could just be metabolites. Are you flame sterilizing the syringe between jars?




This. Without pics, we wont know exactly whats going on.


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OfflineA.k.aM
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Re: Keep getting bacteria?? [Re: LogicaL Chaos] * 1
    #26660374 - 05/09/20 04:24 PM (3 years, 8 months ago)

It’s probably coming from the syringe.


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InvisibleRiverBum
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Re: Keep getting bacteria?? [Re: MuckPeddler] * 1
    #26660382 - 05/09/20 04:29 PM (3 years, 8 months ago)

If you are using half pint jars 1ml per jar is plenty


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OfflineCylarabes
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Re: Keep getting bacteria?? [Re: RiverBum]
    #26660597 - 05/09/20 06:27 PM (3 years, 8 months ago)

If every single jar is going bad every time you can probably blame the syringe.

I'd say try a different syringe, this happened to me my first two grows, I kept getting bacteria in every single jar, thought it was my Tek but when I tried a different syringe I had no contams with the same methods.


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OfflineMuckPeddler
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Re: Keep getting bacteria?? [Re: LogicaL Chaos] * 1
    #26660622 - 05/09/20 06:38 PM (3 years, 8 months ago)





Ok here are two photos these ones have been going for about 2 weeks now! And so even if I heat them up they could still not be sterile? Ive tried 3 needles now dang


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OfflineMuckPeddler
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Re: Keep getting bacteria?? [Re: MuckPeddler]
    #26660657 - 05/09/20 06:58 PM (3 years, 8 months ago)

When there is just small spots of bacteria is it possible to keep growing it?


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OfflineA.k.aM
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Re: Keep getting bacteria?? [Re: MuckPeddler]
    #26660663 - 05/09/20 07:02 PM (3 years, 8 months ago)

Yeah ime with wbs if the myc can still colonize then it’ll fruit. If the bacteria is so bad it stops the myc then it’s done though.


I’m not seeing anything in your jars.


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OfflineMuckPeddler
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Re: Keep getting bacteria?? [Re: A.k.a]
    #26660674 - 05/09/20 07:09 PM (3 years, 8 months ago)

Oh really? maybe im just seeing things idk I thought the yellow globs/slighty yellower areas might be contam lol, ill see what happens then!


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OfflineA.k.aM
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Re: Keep getting bacteria?? [Re: MuckPeddler] * 1
    #26660678 - 05/09/20 07:11 PM (3 years, 8 months ago)

I think they’re duds man two weeks is a long time.


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OfflineMuckPeddler
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Re: Keep getting bacteria?? [Re: A.k.a]
    #26660685 - 05/09/20 07:14 PM (3 years, 8 months ago)

Ya I think so too :thumbdown: :sad: especially since their really isnt any growth at all, time to try again I suppose. Could that be do to temperature or something? Also thanks for the feedback


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OfflineLogicaL ChaosM
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Re: Keep getting bacteria?? [Re: MuckPeddler]
    #26660871 - 05/09/20 08:46 PM (3 years, 8 months ago)

Yeah those are toast. Throw those away.

Looks like u made the mixture too wet.


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OfflineMuckPeddler
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Re: Keep getting bacteria?? [Re: LogicaL Chaos]
    #26661014 - 05/09/20 09:59 PM (3 years, 8 months ago)

Shucks okay ill toss em! And for these ones they are only a few days old but I see some yellow again but does the mixture look too wet? I added less water but maybe its still not good enough and ill add less next time





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OfflineCylarabes
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Re: Keep getting bacteria?? [Re: MuckPeddler]
    #26668355 - 05/13/20 10:06 AM (3 years, 8 months ago)

That does look a little too wet, make sure you're doing 2 parts verm 1 part water 1 part BRF. You might also want to try mixing the verm with the water first and then adding the BRF. Usually my cakes don't have so much wet BRF caked onto the walls of the jar.


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