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Offlinerezasmith
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transferring bacterial mycelium to antibiotic agar - how does it look? * 1
    #26659828 - 05/09/20 11:42 AM (3 years, 9 months ago)

so i had no extra plate left and all my current ones were bacterial and i decided to transfer it to an agar with just a pinch of tetracycline when it was not that hot but not gel either and i stirred it with a metal thingy (flame sterilize before)
anyway this is the result , i think its been cleaned , what is your thoughts?

bacterial culture used for transfer :

agar + tetracycline plate after transfer =


btw i noticed its been slower than usual like by a day - is it because it has antibiotics in it? if i do transfers from this plate would it lose its strength or something?


Edited by rezasmith (05/09/20 11:42 AM)


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OfflineLogicaL ChaosM
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Re: transferring bacterial mycelium to antibiotic agar - how does it look? [Re: rezasmith]
    #26659846 - 05/09/20 11:49 AM (3 years, 9 months ago)

Looks pretty clean to me. That deep container looks like a hassle thou.


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Offlinerezasmith
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Re: transferring bacterial mycelium to antibiotic agar - how does it look? [Re: LogicaL Chaos]
    #26659853 - 05/09/20 11:52 AM (3 years, 9 months ago)

Quote:

LogicaL Chaos said:
Looks pretty clean to me. That deep container looks like a hassle thou.



maybe the picture makes it bigger but i don't think they're that deep , it's 310 ml pp5 containers - i actually do have a batch of unopened petri dishes but honestly this is so much easier (no pour) , since i'm just a beginner i think this works out well for me + the lids are pretty tight too


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OfflineLogicaL ChaosM
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Re: transferring bacterial mycelium to antibiotic agar - how does it look? [Re: rezasmith]
    #26659866 - 05/09/20 11:59 AM (3 years, 9 months ago)

I see it now. Yeah, doesnt look as big as I originally saw. :strokebeard:


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InvisibleLadysKnight
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Re: transferring bacterial mycelium to antibiotic agar - how does it look? [Re: rezasmith]
    #26659877 - 05/09/20 12:03 PM (3 years, 9 months ago)

Hard to tell with the pics, but looks clean.
Once you transfer back to a standard agar recipe, normal growth speed should resume.
You can use glass petris for no-pour, better visibility.
Also, black tea agar works well for getting away from bacteria, but also puts the myc in slow motion.


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Offlinerezasmith
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Re: transferring bacterial mycelium to antibiotic agar - how does it look? [Re: LadysKnight]
    #26659886 - 05/09/20 12:07 PM (3 years, 9 months ago)

Quote:

LadysKnight said:
Hard to tell with the pics, but looks clean.
Once you transfer back to a standard agar recipe, normal growth speed should resume.
You can use glass petris for no-pour, better visibility.
Also, black tea agar works well for getting away from bacteria, but also puts the myc in slow motion.




problem was until now bacteria was my nemesis because i didn't own a good pressure cooker , but i bought one and it arrived just today so i'm all set for the future for the MEA recipe :smile:
edit : btw thanks for the black tea agar tek , never heard of it before seems interesting
and yeah about the picture , one way for me to look for contam is put flashlight on top and bottom and look for anything extra in the plate , usually works


Edited by rezasmith (05/09/20 12:09 PM)


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