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OfflineWestVirginaMan
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New to growing but doing good * 2
    #26658981 - 05/09/20 01:44 AM (4 years, 1 month ago)

So i started out with dried mushrooms spores, from dried mushroom directly, no print.I bought 10 agar dishes. First five i swiped spored and tapped spores onto agar. Only one made it of the first five. I uses that one agar dish and placed a piece of it on the last 5 dished i had, and some brown rice grain jars. So far only one of those dishes were contaminated and none of the jars. I now have 4 fully colonized jars.

For the next part im still waiting on results. I placed two jars in a mono tub layered with a brf/ verm mix. We shall see how this turns out. Anyone interest
ed ask questions. I will post pics if wanted. I haven't seen anyone else do it exactly like this so i figured id put it out there. Also no pc, just stovetop with thermometer.

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OfflineMH5109
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Re: New to growing but doing good [Re: WestVirginaMan]
    #26659007 - 05/09/20 02:09 AM (4 years, 1 month ago)

Wut nutrient media did you  use in your jars inoculated with the agar. Typically people use brf and verm in the pf tek but you don't want brf in your sub you mix the colonized jars into, in your mono or shoebox.


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OfflineWestVirginaMan
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Re: New to growing but doing good [Re: MH5109]
    #26659073 - 05/09/20 02:53 AM (4 years, 1 month ago)

Whole brown rice sterilized at 210° for 1.5hrs. The reason i used brf/verm in my substrate is bc i dnt have coir so i figured verm would be good for the moisture but had no nutrition so that why i figured a brf/ verm mix would do good. I figured it would turn out like a large pf tek cake basically.

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OfflineMH5109
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Re: New to growing but doing good [Re: WestVirginaMan] * 3
    #26659097 - 05/09/20 03:12 AM (4 years, 1 month ago)

Yea but brf has nutrients in it, coir and verm do not. Your substrate you mix spawn with should have little to no nutrients in it so it doesn't grow mold before your mycelium can colonize it. The reason you sterilize brf and verm in a sterile container is to prevent contamination from something else, its not exposed to contaminants until its fully colonized.


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Offlinemushpunx
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Re: New to growing but doing good [Re: WestVirginaMan] * 1
    #26659143 - 05/09/20 03:44 AM (4 years, 1 month ago)

Quote:

WestVirginaMan said:
Whole brown rice sterilized at 210° for 1.5hrs. The reason i used brf/verm in my substrate is bc i dnt have coir so i figured verm would be good for the moisture but had no nutrition so that why i figured a brf/ verm mix would do good. I figured it would turn out like a large pf tek cake basically.





It's really great that you are using agar but your approach here is pretty confused.
Whole brown rice is not suitable to use as spawn like you would rye or oats.
As for what you have going right now, BRF (along with all grains) needs to kept kept sterile from the time it is inoculated until it is fully colonized.
When you spawn to bulk, it is the Spawn that is providing the nutrition.
What you have going on right now is surely going to contaminate, might as well toss it.

Until you have a pressure cooker and can produce grain spawn, I suggest you work with BRF cakes, PF Tek style. But steam sterilize in a pot...
We're you working in a still air box to inoculate your agar?  Even if you were able to get spores from your dried mushroom to germinate, you would absolutely have to transfer the myc away from bacteria and or mold to a new plate. Starting with a dry mushroom, it would most likely take several transfers to maybe get a clean culture.

Post photos of your agar plates and jars, I would like to see them out of curiosity.

I don't mean to sound rude but I believe you are quite confused and have a whole bunch of reading to do before even attempting to start a new project.


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Offlinemushpunx
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Re: New to growing but doing good [Re: mushpunx] * 1
    #26659144 - 05/09/20 03:45 AM (4 years, 1 month ago)

https://www.shroomery.org/forums/showflat.php/Number/24144021

Here, I did you a favor and linked you to Bodhisattva's collection of all the information you could want. Also the Let's Grow Mushrooms videos by RR are a great visual resource. He has a good PF Tek video too.


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OfflineWestVirginaMan
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Re: New to growing but doing good [Re: mushpunx] * 1
    #26659289 - 05/09/20 05:57 AM (4 years, 1 month ago)

Yes i know the process i used is unconventional and ive not seen anyone else do it let alone have success with it. Yes i did use a still air box. I pasturized my verm/brf mix for substrate not sterilized. Its been 3 days and the mycelium has already started to spread. I didnt open my box btw. Just a peak through the lid. Looks like its doing good so far. One thing you said tho.that the grain spawn used is the nutrient. So technically a person could use substrate that has no nutritional value like vermiculite? If i have complete success with this i plan on doing a write up of my process and post it. I know there are alot of people out there who get discouraged bc people say you have to do it certain ways and its just not the case from what ive experienced so far. I will update again when i have some fruiting. Maybe give someone else the encouragement they need.

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OfflineWestVirginaMan
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Re: New to growing but doing good [Re: WestVirginaMan] * 1
    #26659299 - 05/09/20 06:04 AM (4 years, 1 month ago)

And i understand about the verm/brf contaminating. Im worried about that as well. But i guess im gonna find out. Like i said i dont have any coir and only have verm. Could i do a straight verm and add my rice spawn to it that way? And if so how would i pasturize it without a pc. Same method i been using? Pot w/ lid and thermometer.

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Re: New to growing but doing good [Re: WestVirginaMan]
    #26659368 - 05/09/20 06:59 AM (4 years, 1 month ago)

keep goin son! thats some fucking west virginny not giving up right fucking here sports fans.

check out blak cow teks. people are gonna rain kow shit on me for suggesting it but if u cant get coir you got to get somethin. wet cardboard. throw some perlite in to keep it loose. old shoes. something.


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OfflineA.k.aM
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Re: New to growing but doing good [Re: PinkStormtrooper]
    #26659507 - 05/09/20 08:47 AM (4 years, 1 month ago)

Coir has no nutrition its just to hold water, that’s why the amount of mushrooms you can get is based on how much spawn you use.

Hopefully yours works but there’s a good chance it’ll contaminate. If you could use brf in the sub then there would be no point in sterilizing jars of it first.

Anything nutritious and exposed that’s not colonized with myc is a very high risk.


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OfflineWestVirginaMan
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Re: New to growing but doing good [Re: A.k.a]
    #26659589 - 05/09/20 09:27 AM (4 years, 1 month ago)

Maybe my next jar to spawn ill try straight verm. I have some " mushroom compost" i got from the store i could mix with the verm. I was trying to save it for an outside mushroom garden tho lol. And ill post pics of my agar and jars later today. And my monotub if i can get a pic thru the lid.

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OfflineLogicaL ChaosM
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Re: New to growing but doing good [Re: WestVirginaMan]
    #26659644 - 05/09/20 09:48 AM (4 years, 1 month ago)

Very cool. How did you process the brown rice grain? How long did u PC? What strain are you growing?


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OfflineWestVirginaMan
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Re: New to growing but doing good [Re: LogicaL Chaos] * 1
    #26659735 - 05/09/20 10:43 AM (4 years, 1 month ago)

To process the brown rice i first boiled for 5 min then placed in jars an just cooked on stovetop in pot with lid and thermometer at aprox 210° for 1.5hrs. And the strain i have no idea at all lmao. I got the spores from a dried sample. Its a bit longer of a story if u want details lol. Kinda interesting tho.

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Offlinepoisoned
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Re: New to growing but doing good [Re: WestVirginaMan]
    #26659750 - 05/09/20 10:53 AM (4 years, 1 month ago)

Quote:

WestVirginaMan said:
I know there are alot of people out there who get discouraged bc people say you have to do it certain ways and its just not the case from what ive experienced so far. I will update again when i have some fruiting. Maybe give someone else the encouragement they need.



As far as we're aware, you haven't grown a single mushroom yet, so I'm not sure how you can say "its just not the case from what ive experienced so far" if you don't have any.

We want people who come here succeed on their first try instead of experimenting with unconventional methods and getting discouraged when they don't work. Nobody's being discouraged from experimenting when they have a few successful growths under their belt. That's where all the methods we use today came from. When I was reading this forum for the first time ~15y ago, methods were way more complicated and it was way harder to succeed. During that time, mushroom cultivation was basically distilled to very simplified, distilled form, only doing stuff that actually matters. Back in the day, we were inoculating BRF jars in a glovebox or in front of an oven, incubated them and then hoped for the best. Today, bulk methods are way simpler and way more likely to succeed than PF tek was back in the days.

But none of that happened because someone new to cultivation developed those methods on their first try. It's usually people growing consistently good results that developed those methods. You can't even verify it works without doing a comparison to an already proven method.


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OfflineLogicaL ChaosM
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Re: New to growing but doing good [Re: WestVirginaMan]
    #26659871 - 05/09/20 12:00 PM (4 years, 1 month ago)

Very cool. Sounds simple. Yeah, if u wanted to share the story, its up to you. Would be an interesting read :popcorn:

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Re: New to growing but doing good [Re: WestVirginaMan] * 1
    #26660076 - 05/09/20 01:44 PM (4 years, 1 month ago)

You wouldn't use straight verm as a bulk sub. If you are going to do that it's better to put the grains in the tray and use the verm as a casing layer. You can fruit off straight or cased grains, I have done it many times, but you will get better results from spawning to a bulk sub like coir.


You really should read through those links I gave you before attempting anything else. Learn the basics first!

Edited by mushpunx (05/09/20 01:56 PM)

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OfflineWestVirginaMan
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Re: New to growing but doing good [Re: poisoned]
    #26660607 - 05/09/20 06:33 PM (4 years, 1 month ago)

Yes i have not grown a single mushroom yet. But on my first try i have successfully got 4 pint jars of spawn and 3 quart jars of spawn with zero contamination. I know this isnt a breakthrough and i know that anyone who knows what their doing would go about it a different way. So i just want to let it be known im not saying this is a proven method or tek. That the first thing i said was im new. I love any direct help or suggestions. But the reason im doing it this way is bc of certain things i was unable to get my hands on at this time. I may need to do a full write up so there can be alteration to my process instead of poking at the only information given so far.

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OfflineWestVirginaMan
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Re: New to growing but doing good [Re: mushpunx]
    #26660617 - 05/09/20 06:36 PM (4 years, 1 month ago)

Thank you for saying that. I may try this. I dont have any coir but lots of verm but would like to stay away from straight pf tek bc of the low yield. Any other info like this is welcomed. Thank you.

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OfflineWestVirginaMan
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Re: New to growing but doing good [Re: WestVirginaMan]
    #26660632 - 05/09/20 06:46 PM (4 years, 1 month ago)

I wanted to share and ask questions and learn and gain knowledge from others first hand and also share my experience and with hopes that it would possibly help another because we really enjoy everything about it and believe anyone should be able to do this for themselves if that is what they choose to do.

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Offlinemushpunx
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Re: New to growing but doing good [Re: WestVirginaMan] * 1
    #26661237 - 05/10/20 12:01 AM (4 years, 1 month ago)

Quote:

WestVirginaMan said:
Yes i have not grown a single mushroom yet. But on my first try i have successfully got 4 pint jars of spawn and 3 quart jars of spawn with zero contamination. I know this isnt a breakthrough and i know that anyone who knows what their doing would go about it a different way. So i just want to let it be known im not saying this is a proven method or tek. That the first thing i said was im new. I love any direct help or suggestions. But the reason im doing it this way is bc of certain things i was unable to get my hands on at this time. I may need to do a full write up so there can be alteration to my process instead of poking at the only information given so far.





Have you used that spawn yet? Bacteria and mold in spawn jars can be especially difficult to identify even for experienced cultivators. The reason for this is common molds like trichoderma have bright white Mycelium until they sporulate - they will hide in spawn. Once you use that spawn, it's typically when the substrate has been placed into "fruiting conditions" that it will turn green, because you are also placing that mold into fruiting conditions, where it sporulates. The green is the spores it releases upon sporulating. Many times spawn will look clean, substrate will fully colonize and look just fine and..  the mold reveals it'self.
And Bacteria is a whole other story.

Post some photos of your jars.


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Edited by mushpunx (05/10/20 12:05 AM)

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