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OfflineMyco_maiden
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Registered: 05/08/20
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wbs quarts contam??
    #26657259 - 05/08/20 11:44 AM (3 years, 9 months ago)

Started with spider man Tek with a GT strain...broke the fully colonized bag into 11 wbs jars they have been sitting for about 10 days.

Hadn't looked very close for a couple days and I just checked them and now I'm not sure I have healthy myc growing.. I opened one and smelled it and it didn't smell like myc.

Needing insight



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OfflineAmino
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Registered: 05/01/20
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Last seen: 2 years, 10 months
Re: wbs quarts contam?? [Re: Myco_maiden]
    #26657360 - 05/08/20 12:37 PM (3 years, 9 months ago)

Its rather hard to tell in the pictures. What did it smell like? Are the grains too dry or wet? I've had a similar looking issue with WBS before, the grains were too dry, growth was weak and took 2-3 x as long as better hydrated jars containing the same culture.
Is it white or grey? They do look a bit suspect, could be down to environmental factors, contaminant (probably bacterial?), or weak genetics.


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Invisiblewoofwoof
such mushrooms!
I'm a teapot User Gallery


Registered: 01/04/19
Posts: 1,127
Re: wbs quarts contam?? [Re: Amino]
    #26657375 - 05/08/20 12:45 PM (3 years, 9 months ago)

so how or why did you move the grain from bag to jars?


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OfflineMyco_maiden
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Re: wbs quarts contam?? [Re: woofwoof]
    #26657573 - 05/08/20 02:14 PM (3 years, 9 months ago)

@Amino, smelled like wet birdseed. The grain might be too dry. I also added waaaay too much gypsum, so I think that's part of the grittiness. I think it's a weak strain.

@woofwoof, I started with brown rice bags, innoc'ed it and colonized the bag, then I did a grain to grain transfer to the 11 jars of wbs


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OfflineAmino
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Registered: 05/01/20
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Last seen: 2 years, 10 months
Re: wbs quarts contam?? [Re: Myco_maiden]
    #26659371 - 05/09/20 07:05 AM (3 years, 9 months ago)

If it didn't smell sour or mouldy its probably just slow growth. If the grains are too dry there isn't much you can do now ime. I've heard people inject sterile water into their jars if they're too dry, so you could always try that. I shook them for a 2nd time (usually only do one shake) when they seemed to stall at around 80% colonisation, once they'd recovered they were pretty much fully colonised.
I'm sure you've learned this lesson already, but you only need a literal pinch of gypsum per jar.


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