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OfflinePizzamanforty
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Confused with first grow possible contamination
    #26656420 - 05/08/20 12:57 AM (3 years, 9 months ago)

Hey I have started my first grow. One is b+ and the other is supposed to be a+. The b+ spawned and colonized really nicely. The a+ is kind of different. It didn't fully colonize and the pins have color as opposed to being albino. It is also popping up these really small white shrooms with super slim stems. Any help would be welcomed.


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OfflineActionjunkie
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Re: Confused with first grow possible contamination [Re: Pizzamanforty]
    #26656445 - 05/08/20 01:16 AM (3 years, 9 months ago)

Everything I see looks okay. I go straight to fruiting conditions when i pit totes together. Sometimes they colonize then fruit usually they don't.

As far as tall skinny fruit it could be genetics or it could need more fae. You might try fanning more or modifying your monotub to allow for more fae.

Here's a link look at "fruiting conditions"

https://www.shroomery.org/forums/showflat.php/Number/17231313#17231313


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OfflinePizzamanforty
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Re: Confused with first grow possible contamination [Re: Actionjunkie]
    #26656492 - 05/08/20 02:02 AM (3 years, 9 months ago)

Maybe my polyfill on the sides was too much restriction. I'll open it up for some more air. RH in the room is 65% so they should be fine if they are more exposed.


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Offlinemushpunx
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Re: Confused with first grow possible contamination [Re: Actionjunkie]
    #26656494 - 05/08/20 02:07 AM (3 years, 9 months ago)

Your substrates look a bit bacterial.

Anyways,
You should be ignoring room RH when dialing in your tubs. The only RH that matters is at surface level, and you can't measure that. Your bottom poly is too tight if your fruits aren't getting enough FAE, there are giant water drops or puddles on the surface of the sub.
The sub should have a constant layer of tiny water droplets across the surface, that start to dry up just immediately in front of the bottom poly - this is a tub that is dialed in when - you are allowing the max FAE you can without starting to dry the surface more.


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Edited by mushpunx (05/08/20 03:35 AM)


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OfflinePizzamanforty
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Re: Confused with first grow possible contamination [Re: mushpunx]
    #26656504 - 05/08/20 02:17 AM (3 years, 9 months ago)

Which one is bacterial? The fully colonized one? I thought part of it had bacteria but it just looks like a fruit body.


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Offlinemushpunx
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Re: Confused with first grow possible contamination [Re: Pizzamanforty]
    #26656562 - 05/08/20 03:37 AM (3 years, 9 months ago)

The really white ones with the tiny fruits. It's possible it looks like that from lack of FAE, but probably has some bacteria.

How did you inoculate your grains?


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OfflinePizzamanforty
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Re: Confused with first grow possible contamination [Re: mushpunx] * 1
    #26657156 - 05/08/20 10:48 AM (3 years, 9 months ago)

Is that bacteria harmful, it colonized really well. I inoculated rye berry grain bags in a SAB to prevent contamination. Maybe I didn't have enough gas exchange when I switched to tubs.


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OfflineSFS96
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Re: Confused with first grow possible contamination [Re: Pizzamanforty] * 1
    #26657189 - 05/08/20 11:08 AM (3 years, 9 months ago)

Bacteria won’t normally ruin the grow but it can slow stuff down and cause some issues. Iv had some bacterial tubs turn out great though. All u can do is maintain good conditions and hope for the best:thumbup:


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OfflinePizzamanforty
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Re: Confused with first grow possible contamination [Re: SFS96]
    #26657738 - 05/08/20 03:28 PM (3 years, 9 months ago)

Thanks for the advice. They seem to be doing well enough. I know what to change for next time.


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OfflineLogicaL ChaosM
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Re: Confused with first grow possible contamination [Re: Pizzamanforty]
    #26657976 - 05/08/20 05:33 PM (3 years, 9 months ago)

High FAE helps with reducing bacteria growth.


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Offlinemushpunx
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Re: Confused with first grow possible contamination [Re: Pizzamanforty]
    #26658121 - 05/08/20 06:38 PM (3 years, 9 months ago)

Quote:

Pizzamanforty said:
Is that bacteria harmful, it colonized really well. I inoculated rye berry grain bags in a SAB to prevent contamination. Maybe I didn't have enough gas exchange when I switched to tubs.





No I mean what did you inoculate them with?

Spore prints are taken from fruits grown in open air- even when taken from indoor fruits they are never totally clean. And that means the spore syringes that are made from the prints are never going to be 100% clean.

So if you inoculated with a spore syringe, that is likely where your contamination came from -
Spore syringes are usually okay for BRF cakes, they are more forgiving. But grains should really only be inoculated with a clean culture on agar.

Agar gives you the change to germinate hour spores and then transfer clean mycelium away from bacteria and/or mold to a new plate so you have 100% clean inoculant for your grains.


Bacteria , even if it isn't super noticible like in your tubs will lower yields, sometimes substantially.


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OfflinePizzamanforty
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Re: Confused with first grow possible contamination [Re: mushpunx]
    #26658127 - 05/08/20 06:40 PM (3 years, 9 months ago)

I'm working towards that. I have been lurking shroomery and reading obsessively. I bought some petri dishes and agar to give it a go. I just have to wait for it to show up.


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Offlinemushpunx
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Re: Confused with first grow possible contamination [Re: Pizzamanforty] * 1
    #26658368 - 05/08/20 08:07 PM (3 years, 9 months ago)

You are on the right track then!


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OfflineActionjunkie
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Re: Confused with first grow possible contamination [Re: mushpunx]
    #26662241 - 05/10/20 11:58 AM (3 years, 9 months ago)

There's never a "perfect tote" Ive had fruit grow out of crap looking totes. Contamination or even poor fruiting conditions can cause your fruit to grow faster because they are trying to spore before they die. They fruit can become less effective or not at all effective if they are unhealthy. And you certainly wouldn't expect multiple flushes. You got it going okay, you're getting fruit and making adjustments to become closer to perfect. That's about all we can do.


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