Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
OfflineMycopsilo
Green Thumb
Male


Registered: 04/07/20
Posts: 41
Loc: Under RI and MA
Last seen: 1 month, 28 days
Re: What is this Contam on my Agar? [Re: LogicaL Chaos]
    #26656195 - 05/07/20 10:13 PM (3 years, 9 months ago)

Thanks, I have gathered that much information Lmao.


--------------------
Grow it and they will come...:mushroom2: :leaf:
If you want to view paradise, simply look around and view it..


Extras: Filter Print Post Top
OfflineMycopsilo
Green Thumb
Male


Registered: 04/07/20
Posts: 41
Loc: Under RI and MA
Last seen: 1 month, 28 days
Re: What is this Contam on my Agar? [Re: mushpunx]
    #26656220 - 05/07/20 10:23 PM (3 years, 9 months ago)

Got it. Thanks. I will be updating my progress and posting pics as it goes.:mushroom2:


--------------------
Grow it and they will come...:mushroom2: :leaf:
If you want to view paradise, simply look around and view it..


Extras: Filter Print Post Top
OfflineMycopsilo
Green Thumb
Male


Registered: 04/07/20
Posts: 41
Loc: Under RI and MA
Last seen: 1 month, 28 days
Re: What is this Contam on my Agar? [Re: Mycopsilo] * 1
    #26667001 - 05/12/20 04:23 PM (3 years, 9 months ago)

Here is the B+ transfers. I wasn’t thinking when I did it and took the myc from the center... They are looking good though from what I can tell. The first plate I dropped received two pieces of agar by accident. A little sliver fell off of the main transfer. The second plate got a single piece. The second plate has a small dot of what looks like the start of some new myc?






Edited by Mycopsilo (05/12/20 04:31 PM)


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
Trusted Cultivator
Re: What is this Contam on my Agar? [Re: Mycopsilo]
    #26667940 - 05/13/20 04:37 AM (3 years, 9 months ago)

The second plate, that dot could either juSt be a piece of myc that fell off your scalpel or if your wedge rolled, and started growing.

But it also could be a mold colony - mold colonies start out as white spots like that, in places not touching your wedge or myc germination.
That's why we swipe spores onto plates in zig zag patterns, so we have an idea where germination should be and don't take from outside of the pattern.

Plates like that I view as suspect, I take a transfer from the side furthest from the colony, before the myc touches it.
That's what I would do. :shrug:


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineMycopsilo
Green Thumb
Male


Registered: 04/07/20
Posts: 41
Loc: Under RI and MA
Last seen: 1 month, 28 days
Re: What is this Contam on my Agar? [Re: mushpunx]
    #26668086 - 05/13/20 06:52 AM (3 years, 9 months ago)

Ok that’s what I’ll do. On a similar note I made some new Agar plates and did some swipes from my syringes. 5 more B+ and 5 PE. I poured the agar at 120F and I still got condensation.. What the hell is up with that? Every post I read said it starts to solidify at 120F but that wasn’t the case. So I noticed the agar was still liquified at 100F so I guess I will pour at 100 from now on. I don’t think My IR thermometer is off.


--------------------
Grow it and they will come...:mushroom2: :leaf:
If you want to view paradise, simply look around and view it..


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
Trusted Cultivator
Re: What is this Contam on my Agar? [Re: Mycopsilo]
    #26668152 - 05/13/20 07:50 AM (3 years, 9 months ago)

You could go cooler if it's still causing problems. I eyeball mine a lot of the time, when it's just about going to solidify if I leave it any longer, I pour. Never get condensation.


I usually only do one or two germination plates of each variety because I'm just gonna looking to start one one culture of each. Saves plates


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineMycopsilo
Green Thumb
Male


Registered: 04/07/20
Posts: 41
Loc: Under RI and MA
Last seen: 1 month, 28 days
Re: What is this Contam on my Agar? [Re: mushpunx]
    #26668198 - 05/13/20 08:19 AM (3 years, 9 months ago)

Ok. So you you just transfer off of those 2 plates to make more plates? How do you isolate? You just transfer off until you have a culture that is completely uniform?


--------------------
Grow it and they will come...:mushroom2: :leaf:
If you want to view paradise, simply look around and view it..


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* s juvec 140 9 05/13/20 07:24 AM
by juvec
* How to make agar 2 agar transfer? esp 6,677 7 01/08/04 11:08 AM
by YidakiMan
* agar transfers *DELETED* thisone 1,246 3 11/20/03 08:58 PM
by Mycomancer
* Innoculation with agar wedge Visigoth 5,296 6 01/15/23 12:59 PM
by Screwup
* Re: mold in agar plate Anonymous 12,258 3 12/04/99 12:35 AM
by Anonymous
* Opinions about which agar plates should be transferred *DELETED*
( 1 2 all )
mr.s420 682 20 05/25/20 02:08 PM
by Domno
* Re: Contams in Honey Water Lana 924 2 07/16/00 07:30 AM
by Hippie3
* Re: Why do I want to grow mycelium on Agar?? Anonymous 2,476 3 02/13/00 04:07 PM
by AnubisRonin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
714 topic views. 33 members, 180 guests and 59 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.006 seconds on 13 queries.