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OfflineAstoriensis
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Registered: 04/20/17
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26642068 - 05/01/20 07:38 PM (3 years, 9 months ago)

Yeah the prices on these keep going up glad I grabbed a few packs already


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InvisibleMr. Alien
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Posts: 6,290
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Astoriensis]
    #26642095 - 05/01/20 07:59 PM (3 years, 9 months ago)

Wow i didn't know they increased the price. Time to update the OP, it was 12$ before.


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InvisibleMr. Alien
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Registered: 01/14/14
Posts: 6,290
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26642173 - 05/01/20 08:49 PM (3 years, 9 months ago)



I already pressed the helpful button :lol:

The downside that he mentions, i mean compared to disposable petridishes or glass petridishes yeah... but if you see them with the right light they are pretty much clear enough to see every section of the mycelium.

These photos were provided by Eatualive

Quote:

eatyualive said:
So far loving the plates! :thumbup:






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Offlinemicrodose83
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Registered: 12/31/19
Posts: 467
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Hobbit GDF]
    #26642289 - 05/01/20 10:01 PM (3 years, 9 months ago)

Quote:

Hobbit GDF said:
Quote:

microdose83 said:
Quote:

Hobbit GDF said:
Quote:

Hobbit GDF said:
So my mail got here today. Here's the 8 from Amazon on left. The ones on the right (40pc) are from a plastic company. The visibility on them isn't as clear but it's still pretty good. I can always open in the flow.


I'm waiting for some different agar. I'm not going to keep using telephone brand. Been having issues.



The amazon link you just showed are like these blurry one I got from another company.



Do you have the info on the plastic company you went through?  Id be interested in comparing prices of them. Especially since the place I get my dishes screwed me over lol



Its was Berlin packaging. They are not good. They aren't clear they are foggy. They seal shut in PC and seal to each other. Like when you stack them they suction together. They lids suck and seal. Extremely hard to pry open if not left half loose in the PC. Dont buy them from berlin



Oh damn! Haha. Hell thanks for the rating!  I'll definitely not buy from them!


--------------------
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🤘😜🤘  Too weird to live, too rare to die!" 🤘😜🤘


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Offlinejackrabbitcytoplas
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Registered: 01/30/20
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: microdose83] * 1
    #26647887 - 05/04/20 09:39 AM (3 years, 9 months ago)

These pins have gotten pretty big in the HG with the lid closed tight since inoculation.
They seem taller than the top now because of bottom watering.
Just thought I'd share.



Originally meant to take clean pins to agar but the brf puck showed blue/green mold around the uncolonized edge at 70%, eventually the mycelium overtook the mold and pinned but I wasn't sure I could trust the pins anymore.

BTW,
How do you guys clean these between uses?
I PC them closed (so I don't spread shit in the air), wait for them to cool, then open them over the trash and scrape everything with a toothpick, then rinse them in the sink until they are visibly completely clean, load with new agar and PC.
I just had a bunch of sterilized plates go bacterial 4 days after sterilization without opening them and I suspect that it's because I didn't clean them well enough because previously I used soap.


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Invisiblestaytrippy420
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Registered: 03/23/13
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jackrabbitcytoplas]
    #26647940 - 05/04/20 10:02 AM (3 years, 9 months ago)

I just dump them and wash with hot soapy water. Refill, pc, repeat.

Did you vent your pc for 10 minutes before you ran them?


--------------------
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LAGM2020


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Offlinejackrabbitcytoplas
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Registered: 01/30/20
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: staytrippy420]
    #26648288 - 05/04/20 12:37 PM (3 years, 9 months ago)

Yeah, sure. I've kept everything the same except for not using soap this time.
Wanted to see what other people do before trying again without it.


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InvisibleMr. Alien
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Registered: 01/14/14
Posts: 6,290
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jackrabbitcytoplas]
    #26648990 - 05/04/20 07:23 PM (3 years, 9 months ago)

Quote:

jackrabbitcytoplas said:
Originally meant to take clean pins to agar but the brf puck showed blue/green mold around the uncolonized edge at 70%




how long you sterilize the HGBRF cakes? Did you sterilize them inside a mason jar/outer container? I was about to make that experiment myself. HGBRF is a great idea. I'd like to imagine a world in which noobs can just use HG for everything.


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Offlinejackrabbitcytoplas
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Registered: 01/30/20
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26649469 - 05/05/20 12:50 AM (3 years, 9 months ago)

Quote:

Mr. Alien said:
Quote:

jackrabbitcytoplas said:
Originally meant to take clean pins to agar but the brf puck showed blue/green mold around the uncolonized edge at 70%




how long you sterilize the HGBRF cakes? Did you sterilize them inside a mason jar/outer container? I was about to make that experiment myself. HGBRF is a great idea. I'd like to imagine a world in which noobs can just use HG for everything.




I only prepped one using the leftover mix from a batch of 1/4 pints I made, so no jar or container. Had it sitting on top of the rest of the stuff.
I did it hoping to get quick colonization and pins for transfer. The HG are pretty small though, so I'm not sure about getting meaningful yields from them on their own.

I plan to do straight brown rice cakes/petris with these eventually, and I'd like to inoculate with a LC/LI, trying to figure out a minimal grow schedule for personal use.
It will have to wait though because I can't muster up the motivation to do the bare minimum right now, already let a bunch of stuff go to waste because of it.


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Offlinebrikogen
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Registered: 04/06/20
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jackrabbitcytoplas] * 1
    #26652183 - 05/06/20 04:54 AM (3 years, 9 months ago)

With the price hike I was looking for alternatives. I found that ikea sells PP containers for $1,50. To find them on your regional IKEA website just search for this number "392.691.01". They are not screw top but are airtight.



Edited by brikogen (05/06/20 04:56 AM)


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InvisibleFooMan
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26652330 - 05/06/20 06:52 AM (3 years, 9 months ago)

Quote:

Mr. Alien said:
Quote:

DharmaForKarma said:
Hey everybody! First post. 

Already loving these plates for my first tries with agar, eight leetle babies all tucked in on their shelf. 

I didn't see this mentioned: There's a new kid in town on Amazon, selling twenty for about 60% less than the other guy.  I just ordered a pack, hopefully they're the same thing.

Link:  (thanks nevereataaminita)

https://www.amazon.com/gp/product/B083HXXB34




They have worst visibility! Look at the last picture of the amazon link! the lid windows is blurry. Although they seems to be pp5, so probably safe on that side. But i wouldn't buy them cause of the visibility:



Only reward the companies that sells a worthy product.






No, they're the same. I got a plate from eats that was from the original seller you posted awhile back. I ordered the Amersumer ones afterwards and comparing them side by side they are identical.


Here's an actual pic:




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Offlinejackrabbitcytoplas
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Registered: 01/30/20
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: brikogen]
    #26652335 - 05/06/20 06:56 AM (3 years, 9 months ago)

Quote:

brikogen said:
With the price hike I was looking for alternatives. I found that ikea sells PP containers for $1,50. To find them on your regional IKEA website just search for this number "392.691.01". They are not screw top but are airtight.






You'll probably need to modify them to allow pressure to escape while in the PC or they will warp/explode.


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Offlinebrikogen
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Registered: 04/06/20
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jackrabbitcytoplas]
    #26652440 - 05/06/20 07:57 AM (3 years, 9 months ago)

Quote:

jackrabbitcytoplas said:
Quote:

brikogen said:
With the price hike I was looking for alternatives. I found that ikea sells PP containers for $1,50. To find them on your regional IKEA website just search for this number "392.691.01". They are not screw top but are airtight.






You'll probably need to modify them to allow pressure to escape while in the PC or they will warp/explode.




I have a large variant of this pot and lid which I use to cultivate water kefir. A byproduct of that is the buildup of CO2 in the container. But even though the seal is water and airtight if the pressure is too high it pushes the lid up enough to outgas on its own and seal back up. I can also confirm this when the lid is closed and I slightly pull on the lid, the container "burps" by releasing a small hissing sound. So it sort of is a built in one way release valve. It's a much safer option than using screwed cap containers for that application. As it can make the bottle explode if left unattended for a while.


Edited by brikogen (05/06/20 08:01 AM)


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Offlinejackrabbitcytoplas
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: brikogen]
    #26652480 - 05/06/20 08:33 AM (3 years, 9 months ago)

Quote:

brikogen said:
Quote:

jackrabbitcytoplas said:
Quote:

brikogen said:
With the price hike I was looking for alternatives. I found that ikea sells PP containers for $1,50. To find them on your regional IKEA website just search for this number "392.691.01". They are not screw top but are airtight.






You'll probably need to modify them to allow pressure to escape while in the PC or they will warp/explode.




I have a large variant of this pot and lid which I use to cultivate water kefir. A byproduct of that is the buildup of CO2 in the container. But even though the seal is water and airtight if the pressure is too high it pushes the lid up enough to outgas on its own and seal back up. I can also confirm this when the lid is closed and I slightly pull on the lid, the container "burps" by releasing a small hissing sound. So it sort of is a built in one way release valve. It's a much safer option than using screwed cap containers for that application. As it can make the bottle explode if left unattended for a while.




Pretty cool that it works for fermenting.
In the PC you also have to overcome the pressure of the PC in order to burp, not just the atmospheric pressure. 
Also the lid might collapse while it's cooling.
But there's only one way to find out.


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Offlinebrikogen
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Registered: 04/06/20
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jackrabbitcytoplas]
    #26652588 - 05/06/20 09:36 AM (3 years, 9 months ago)

Quote:

jackrabbitcytoplas said:
Quote:

brikogen said:
Quote:

jackrabbitcytoplas said:
Quote:

brikogen said:
With the price hike I was looking for alternatives. I found that ikea sells PP containers for $1,50. To find them on your regional IKEA website just search for this number "392.691.01". They are not screw top but are airtight.






You'll probably need to modify them to allow pressure to escape while in the PC or they will warp/explode.




I have a large variant of this pot and lid which I use to cultivate water kefir. A byproduct of that is the buildup of CO2 in the container. But even though the seal is water and airtight if the pressure is too high it pushes the lid up enough to outgas on its own and seal back up. I can also confirm this when the lid is closed and I slightly pull on the lid, the container "burps" by releasing a small hissing sound. So it sort of is a built in one way release valve. It's a much safer option than using screwed cap containers for that application. As it can make the bottle explode if left unattended for a while.




Pretty cool that it works for fermenting.
In the PC you also have to overcome the pressure of the PC in order to burp, not just the atmospheric pressure. 
Also the lid might collapse while it's cooling.
But there's only one way to find out.




IKEA should reopen in about a week around here :grin:


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Offlineramthroughram
Junior Mushie Engineer


Registered: 11/20/19
Posts: 91
Last seen: 5 months, 10 days
Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: FooMan]
    #26652897 - 05/06/20 12:48 PM (3 years, 9 months ago)

Quote:

FooMan said:
No, they're the same. I got a plate from eats that was from the original seller you posted awhile back. I ordered the Amersumer ones afterwards and comparing them side by side they are identical.


Here's an actual pic:







Thanks for confirming and posting a pic! Going to purchase some and if for some reason my batch are blurry I can always do a return.

---

Got a lovely PE culture going right now


Beautiful, love the plates, thank you Alien!


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InvisibleMr. Alien
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Registered: 01/14/14
Posts: 6,290
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: FooMan]
    #26653533 - 05/06/20 06:37 PM (3 years, 9 months ago)

Quote:

FooMan said:
No, they're the same. I got a plate from eats that was from the original seller you posted awhile back. I ordered the Amersumer ones afterwards and comparing them side by side they are identical.





Great, if enough people confirm that they are the same, I'll add it in the OP.


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InvisibleHobbit GDF
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #26653873 - 05/06/20 08:59 PM (3 years, 9 months ago)

I bought some today. I'll let you know asap. I'm starting to pour disposable plates now too. Love it. Love both.


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OfflineDharmaForKarma
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: DharmaForKarma]
    #26655369 - 05/07/20 03:18 PM (3 years, 9 months ago)

This pic shows a container from the original seller (top, green X) and one from the new source I linked.  Lighting isn't great but they're pretty similar.



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OfflineOdo
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: DharmaForKarma]
    #26655962 - 05/07/20 08:11 PM (3 years, 9 months ago)

Hi all.  I'm happy to say I bought 24 holy grail plates from Beebeecraft for $30.50, including shipping.  They came from china and only it took a few weeks. I finally prepped the plates a few days ago and made transfers of some clone plates which I started in whatever PP5 cups I could find, given the pandemic.  It was a while ago that I did the original agar clones... I had several pins that I transferred to the HG plates.  Thanks for the awesome tek, Mr. Alien!

I don't know if I'm the first one to think of the following way to get rid of condensation FAST. If so, I'm happy I that I could contribute to the forum from which I have gained so much knowledge.  Here goes...

As stated in the tek, the top plates in the stack have some condensation in them.  I had 24 total, so 12 with and 12 without. Instead of waiting a couple weeks, I thought why not use the dehydrator to suck the moisture out.  I put the 12 HG plates with heavy condensation in the dehydrator and set it to around 110F over night.  It looked like this when they went in:


In the morning most of the condensation was gone. 


I turned it down to the lowest setting 105F and a few hours later all the condensation was gone.  I let it sit for a while but when I looked at it later, much of the condensation had returned.


Not to be deterred, I put the dehydrator back on for a few hours at 105F again until the condensation disappeared, then I left the lid (the part with the fan and heater) slightly ajar so less heat transfer occurred, and ran it for another couple hours.  The result was very dry plates in about a day from original PC, instead of a couple weeks! 


I had the lids of the plates slightly loose during the entire reheating process.  I will continue to monitor whether the contamination rates are different between the original low to no condensation plates and the Odo-dry plates... Hopefully not.  One more thing:  By the time I got all the plates prepped and into the SAB for the transfers, some clouding of the lid did re-occur.  However, it was very light and cleared up by the next day.  I hope this works for others who have come to love Alien's Holy Grail plates.


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