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OfflineTzooman
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Firsrt agar tek and spore transfer feedback
    #26654786 - 05/07/20 10:01 AM (3 years, 9 months ago)



First agar tek for me.  Direct spore transfer (P. cubensis) to MYA agar.  inoculation date April 23, 2020.  Stored at 21C.  How do they look and at what point should I consider transferring to more agar plates?


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OfflinePinkStormtrooper
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Re: Firsrt agar tek and spore transfer feedback [Re: Tzooman]
    #26655255 - 05/07/20 02:01 PM (3 years, 9 months ago)

theres some stuff to work with there for sure but thats way slow on a dish. where did you keep them? I keep mine on top of a t5 light and you can see growth by the day.

edit - just americanized 21C is 69deg warm those lil dudes up to about 80, err 26- 27 C.


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Edited by PinkStormtrooper (05/07/20 02:03 PM)


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InvisibleRoger Clemency
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Re: Firsrt agar tek and spore transfer feedback [Re: PinkStormtrooper] * 1
    #26655267 - 05/07/20 02:06 PM (3 years, 9 months ago)

They may have taken a little while to germinate and then grown at a normal rate. They don’t look to far behind what I’d expect.

They be ready for transfers OP. Pick a few spots that seem to have strong, orderly growth and transfer to a few new plates.


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OfflineTzooman
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Re: Firsrt agar tek and spore transfer feedback [Re: PinkStormtrooper]
    #26655740 - 05/07/20 06:24 PM (3 years, 9 months ago)

Roger that!  I will turn up the heat on the transfers.


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OfflineTzooman
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Re: Firsrt agar tek and spore transfer feedback [Re: Tzooman]
    #26681611 - 05/19/20 06:03 PM (3 years, 8 months ago)

Temperature was definitely an issue.  Ramped up to 78F.  Transferred some good looking growth (x4) to new plates.  Grew them for 5 days and then inoculated 4 rye grain jars.  Some of the other un-isolated plates looked great too after the heat increased.  Used sections of two of those for 2 more jars.  6 jars on the go.  Will see if the isolated strains make any difference.

Keeping the grain at the same temp.


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