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OfflineJbrady5555
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Agar to grain noob questions
    #26652758 - 05/06/20 11:28 AM (3 years, 9 months ago)

Okay so I’m onto my second agar to agar transfer but this time I didn’t scrap the transfer one plates. I want to use a few of the really clean ones to gain experience with colonizing grain jars, which will then gain me experience making a coir substrate. I’ll use shoe boxes for this. I know I haven’t done enough transfers yet but I feel the experience will be worth it and who knows, maybe a few shoeboxes actually work out.

How big of a wedge do you use to knock up a grain jar? Small just like agar to agar? Just one or multiple wedges?

I’m going to include the same pics from a thread from earlier today, I threw these jars away today, the ones I’m going to use are more uniform without the questionable growth away from the myc. How would you decide where to take a wedge from for agar to grain with these plates? Thanks for any info.






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InvisibleHtaeh
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Re: Agar to grain noob questions [Re: Jbrady5555]
    #26652780 - 05/06/20 11:41 AM (3 years, 9 months ago)

Quote:

Jbrady5555 said:
Okay so I’m onto my second agar to agar transfer but this time I didn’t scrap the transfer one plates. I want to use a few of the really clean ones to gain experience with colonizing grain jars, which will then gain me experience making a coir substrate. I’ll use shoe boxes for this. I know I haven’t done enough transfers yet but I feel the experience will be worth it and who knows, maybe a few shoeboxes actually work out.

How big of a wedge do you use to knock up a grain jar? Small just like agar to agar? Just one or multiple wedges?

I’m going to include the same pics from a thread from earlier today, I threw these jars away today, the ones I’m going to use are more uniform without the questionable growth away from the myc. How would you decide where to take a wedge from for agar to grain with these plates? Thanks for any info.





Well I'm only just a little further ahead of you with delving into this side of cultivation so I can give you some of what I've learnt over the past couple weeks.

Firstly, to noc up grain with agar technically any amount will do, the bigger the chunk the quicker it will jump to the grain and colonise. I agree that trying your hand (as long as you're prepared that it could all go tits up) at some grain prep and noccing with agar plates that probably have not been transferred enough to gain some experience; I currently have a shoebox colonising after spawning an ms agar plate to grain (so 0 transfers) and that both looked healthy in the jar and now colonising coir, I did it for the same reason as your thinking of doing it, experience, and so far its been rewarding and its even better to know there are potential fruits from the effort put in.

In regards to deciding where to cut from a plate to transfer I confess that I've been rather random and taken from all over (clean plates), this is due to working with a small SAB atm with poor visibility and work space but theoretically you want to be taking a grain of rice size cutting of agar on the leading edge of the growth furthest away from any contams if they're present.

Really hoping for you to have success from your endeavours!


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OfflinePMBastian
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Re: Agar to grain noob questions [Re: Jbrady5555]
    #26652792 - 05/06/20 11:45 AM (3 years, 9 months ago)

I think people in general use plates that are almost fully grown (but not all the way to the edge) to knock up grain. I use grain bags which you can fit more than 4 quarts into, and i use half a plate or a whole plate to one bag. But with jars i would say you should use one plate to one "batch" of jars, i.e. as many jars as you are planning to fruit in the same tub.

But as the other reply says, you will be able to noc up grain with even a tiny wedge, if your sterile tek is good it should work.

I crush my plates inside the bag after i've nocced and sealed it, to create more inoculation points and speed things up. But with jars i would only use one wedge, to minimize the number of times you have to cut the agar with the scalpel. Each cut is a new possible vector for contam.


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OfflineOne of Us
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Re: Agar to grain noob questions [Re: Jbrady5555]
    #26652801 - 05/06/20 11:49 AM (3 years, 9 months ago)

Where you want to take transfers from is the exact same places people suggested in your other thread.

It doesn't matter if you are transferring to agar or to grain, in either case you want the healthiest growth.

Size of the wedge to put to grain doesn't really matter much. Taking small wedges will allow you to inoculate more jars, but they will take longer to colonize. The opposite is true of larger wedges: less jars can be inoculated, but they will colonize faster. Using multiple wedges per jar will definitely speed things up as well


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OfflineJbrady5555
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Re: Agar to grain noob questions [Re: One of Us]
    #26652822 - 05/06/20 11:59 AM (3 years, 9 months ago)

Quote:

One of Us said:
Where you want to take transfers from is the exact same places people suggested in your other thread.

It doesn't matter if you are transferring to agar or to grain, in either case you want the healthiest growth.

Size of the wedge to put to grain doesn't really matter much. Taking small wedges will allow you to inoculate more jars, but they will take longer to colonize. The opposite is true of larger wedges: less jars can be inoculated, but they will colonize faster. Using multiple wedges per jar will definitely speed things up as well





I guess that’s one of the main things I’m trying to wrap my head around, what is the healthiest growth on the plate. Is it the most dense fuzzy stuff or more so the strandy veiny looking stuff. Thank you all.


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OfflinePMBastian
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Re: Agar to grain noob questions [Re: Jbrady5555]
    #26652838 - 05/06/20 12:09 PM (3 years, 9 months ago)

as far as i know, no one really knows. both rhizo and tomentose myc can fruit really well. or really badly. It doesn't seem like mycelium appearance is a good indicator of what genetic attributes the culture has.

I'm no expert, this is mostly info i regurgitate from a lot of reading on this forum.


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OfflineOne of Us
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Re: Agar to grain noob questions [Re: Jbrady5555] * 1
    #26652848 - 05/06/20 12:14 PM (3 years, 9 months ago)

fuzzy (known as tormentose) or strandy (known as rhizomorphic) doesn't matter much. People have a slight preference for rhizo(strandy) growth because it is unmistakably mycelium. Tormentose growth can sometimes be difficult to differentiate from contams, especially for new growers. Also rhizo growth tends to grow faster and is more aesthetically pleasing.

You want the growth to look organized, like its working together if that makes sense.
You also want to avoid areas like the bottom portion of plate one where the growth is translucent. Although, this can sometimes be caused by really soft agar, and the mycelium grows under the surface.


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InvisibleHtaeh
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Re: Agar to grain noob questions [Re: Jbrady5555]
    #26652852 - 05/06/20 12:16 PM (3 years, 9 months ago)

AFAIK fuzzy growth can be a lot of genetics growing upon one another, hence transferring more and more to further isolate the genetics at play, as well as checking for hidden contams. It was my understanding, albeit potentially incorrect that rhizomorphic growth is the myc searching for nutrition and selecting the veiny (rhizomorphic) growth will give you quicker colonisation(?). As mentioned above either two types of myc growth will produce equally well in regards to fruiting.


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OfflineJbrady5555
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Re: Agar to grain noob questions [Re: Htaeh]
    #26652875 - 05/06/20 12:27 PM (3 years, 9 months ago)

This is the info I’ve been looking for. Thank you all


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OfflineBrian Jones
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Re: Agar to grain noob questions [Re: Jbrady5555]
    #26652879 - 05/06/20 12:29 PM (3 years, 9 months ago)

I make 7 grain jars at a time. If the plate is nearly to the edges, I get 7 wedges from one plate. If the colonization on the plate is smaller, I use two petri's.


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