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OfflineAbabyphoenix
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Registered: 03/21/19
Posts: 1,015
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Last seen: 24 days, 23 hours
How do you fuck up PastyPlates?
    #26646886 - 05/03/20 07:53 PM (3 years, 8 months ago)

Need a bit of advice from my grow along, here she is.:super:
Quote:

Ababyphoenix said:


5/3 Someone done poisoned the water hole!
Ok so... My agar prep has to be wrong or something because most of my agar dishes exploded with mold but not on the surface, on the underside of the agar puck?

This was taken out of the fridge 2 days...!

I'm not sure what to do here kinda sad ): But good news is not all my plates did this? Some have been out of the fridge way longer than the ones that have exploded and look crystal clear... but no myc growth either over 10 days now..
From 4/22!

I know for a fact something went wrong with my agar because some of the plates came out as water? I didnt cook it hot enough before filling the plates so i think it didnt homogenize correctly and some plates got water with some agar and some got a ton of agar :shrug: Idk that's just a guess but some of my plates came out as water after PC for 45 min so there's gotta be something up.

Anyway since i made so many plates one eventually got myc growth without the contam under the puck, check it out. First myc on agar :rockon:
As you can see i already went to absolute town on it making transfers yesterday when i realized my agar dishes were blowing up on my and wanted to get the myc away from that plate asap since it still looked good beneath, and even if it didnt i tried my best not to cut all the way down through the puck. There's also obviously a few completely trash dishes with slime mold, i just wash them out re prep and try again correct?


Made about 5 transfers off that into new dishes and hopefully we can get some new agar plates made today and recover from this stumble. The three dishes i actually cooked a bit before i poured are crystal clear. I did this cause i felt like the agar media should've been thick as i poured it which it was once i cooked it together... so 3 of the 24 should be good...
So far 7 plates are out with a mold , or water.
:waitthatsbad:  :bestcry:
We still got 9 that look good tho :crazy2:

Questions: 1.) I'm pretty sure i did my agar prep wrong, can anyone agree?
2.) Is all my agar work ruined or can i make fresh plates and keep transfering? Or just start new from spore prints?
3.) trash dishes with slime mold, i just wash them out re prep and try again correct?
Any advice would be much appreciated, thanks everyone :pipesmoke2:




--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:



Edited by Ababyphoenix (05/17/20 07:04 AM)


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: First time Agar: Contam, and weird dishes. [Re: Ababyphoenix]
    #26646899 - 05/03/20 08:01 PM (3 years, 8 months ago)

I’m confused.
Your inoculating then putting directly in the fridge?


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OfflineAbabyphoenix
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Registered: 03/21/19
Posts: 1,015
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Re: First time Agar: Contam, and weird dishes. [Re: Strainsfordaze]
    #26646912 - 05/03/20 08:06 PM (3 years, 8 months ago)

Quote:

Strainsfordaze said:
I’m confused.
Your inoculating then putting directly in the fridge?




Oh no no, I prepped 24 Pasty's and kept most in the fridge till i needed them. I kept the inoculated ones in my SAB.
inoculated with spore syringe for most but.. the one that grew (Pictured) was from my spore prints. IF you want more info here's the whole log

The spore syringe just grew a bunch of slime on many plates, that or very slimey looking myc...  :tryingnottodie:

Nice name gradient by the way  :like:


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OfflineAbabyphoenix
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I'm a teapot User Gallery


Registered: 03/21/19
Posts: 1,015
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Last seen: 24 days, 23 hours
Re: First time Agar: Contam, and weird dishes. [Re: Ababyphoenix]
    #26676269 - 05/17/20 05:15 AM (3 years, 8 months ago)

I feel absolutely stupid guys. I've ruined a batch of pasty plates not once but twice now and i have no clue how, hope you guys can please explain my issue here.

So i started back and have been following PastyPlates easy agar tek, the one your not supposed to be able to fuck up right?


And many more...
WRONG!

I chalked my first batch up to a bad first run, eye-balled the mix, new to my cooker and agar work in general so i had some room for error. But this second time i measured out roughly 5g of this Malt Extract Agar from shroomsupply which I was told and am under the impression i just mix with water, so i mixed the recommended (1L. to 20-25g mix) 1/4 liter bottled water to be "extra safe" this time...
I cooked it to homogenize and poured my plates (after i washed them with dish soap, maybe should have used my bleach solution?). I didn't change the micropore tape, is that where i went wrong? This would be the second run for these plates WAIT WHY DIDNT I CHANGE THE MICROPORE TAPE. Anyway. I didnt think itd matter seeing as i was going to PC the fucking micropore tape, it'd be sterile.
Foiled PC'ed for 45 mins above 15 PSI and boom they grew mold in 2 days.

Long story short i just cooked up 12 batches of mold soup i think. They look slightly the same as the pictures above. These plates arent green just lots of dots on the bottom... I'm kinda heart broken here any advice would be cool cause idk what to do different at this point. I got some healthy plates with some healthy culutes somehow but not many... I might have to throw away almost all of 5/15's work.


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:



Edited by Ababyphoenix (05/17/20 05:36 PM)


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OfflineAbabyphoenix
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I'm a teapot User Gallery


Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 24 days, 23 hours
Re: First time Agar: Contam, and weird dishes. [Re: Ababyphoenix]
    #26677320 - 05/17/20 05:23 PM (3 years, 8 months ago)

Quote:

Ababyphoenix said:
Quote:

A.k.a said:
lol it is but you have to actually pour some in first.




Pour some in what? Those are prepped pasty plates, blue agar so it might look like theres nothing in them?

Quote:

ouuwee said:
Are you talking about those remnants on the bottom? I don't think they are mold but are actually remnants from agar/lme that wasn't mixed. I have the same thing









I dont think it's remnants but it would make my day if it was. Nothing i mixed up was green ):
shine a light through the top of ur agar dish and see if the remnants show up better? I can't see what you got there very well.

Here are 3 of my 10+ plates that look like this. Please let me know if its just remnants, i didnt think mold would germinate as quick as a day and a half or 2 out of PC..









Now that remanents have been mentioned i feel a bit more at ease here, can anyone confirm here? ALl these dots dont strike me as mold but i have no clue really, am new to making newt plates for shit to grow on hehe :grin:


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:



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