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hazyhorse
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Registered: 03/19/19
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forgot to add honey to agar plates...?
#26646183 - 05/03/20 02:38 PM (3 years, 8 months ago) |
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hey all,
iβm PC some pastyplates i made up last night & realized i forgot to put a drop of honey in the mix. can i still reliably use these plates? or should i scrap the agar & make a new batch?
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mushpunx
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Re: forgot to add honey to agar plates...? [Re: hazyhorse]
#26646205 - 05/03/20 02:50 PM (3 years, 8 months ago) |
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What was your agar recipe? If it's Potato Dextrose Agar I usually add in a little corn syrup or honey. Malt Extract Agar I don't.
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hazyhorse
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Re: forgot to add honey to agar plates...? [Re: mushpunx]
#26646211 - 05/03/20 02:54 PM (3 years, 8 months ago) |
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iβm using PDA a la pastywhytes agar recipe! i totally meant to add some in, i just always almost forget (& actually forgot this time)
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mushpunx
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Re: forgot to add honey to agar plates...? [Re: hazyhorse]
#26646218 - 05/03/20 02:58 PM (3 years, 8 months ago) |
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If it was me, I'd just make a new batch of agar. You could probably use it. Maybe wait for someone else, I pretty much only use MEA.
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hazyhorse
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Re: forgot to add honey to agar plates...? [Re: mushpunx]
#26646224 - 05/03/20 03:01 PM (3 years, 8 months ago) |
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gotcha. yeah, itβs not really too inconvenient to just mix up a new batch. just figured iβd ask before either using them or starting over. thanks for the quick responses!
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poisoned
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Re: forgot to add honey to agar plates...? [Re: hazyhorse]
#26646391 - 05/03/20 04:12 PM (3 years, 8 months ago) |
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I've used straight PDA in the past and it worked.
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hazyhorse
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Re: forgot to add honey to agar plates...? [Re: poisoned]
#26646458 - 05/03/20 04:38 PM (3 years, 8 months ago) |
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Quote:
poisoned said: I've used straight PDA in the past and it worked.
did you notice any difference in growth?
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HamHead
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Re: forgot to add honey to agar plates...? [Re: hazyhorse]
#26646483 - 05/03/20 04:48 PM (3 years, 8 months ago) |
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If there's potato flakes, should be good to go.
I used to add karo but after getting good results with just potato flakes, that's all the nutrients I use anymore.
-------------------- The Italian researchersβ findings, published by the INTβs scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February. https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF This online first version has been peer-reviewed, accepted and edited, but not formatted and finalized with corrections from authors and proofreaders https://www.icandecide.org/
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hazyhorse
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Re: forgot to add honey to agar plates...? [Re: HamHead]
#26646629 - 05/03/20 05:53 PM (3 years, 8 months ago) |
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good to know! i think iβll just grow out these plates then. i just wasnβt sure how integral the honey/karo was for the myc. thanks again!!
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LoadedFish
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Re: forgot to add honey to agar plates...? [Re: hazyhorse]
#26953595 - 09/24/20 10:34 PM (3 years, 4 months ago) |
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This just happened to me. Did your plates turn out okay?
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hazyhorse
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Re: forgot to add honey to agar plates...? [Re: LoadedFish]
#26953778 - 09/25/20 01:38 AM (3 years, 4 months ago) |
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yep they were totally fine! i still occasionally forget the honey. the myc grows all the same.
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Liberto
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Re: forgot to add honey to agar plates...? [Re: hazyhorse]
#26953827 - 09/25/20 03:43 AM (3 years, 4 months ago) |
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I know you can heat up agar to make it fluid again. Then add honey and sterilize again?
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hazyhorse
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Re: forgot to add honey to agar plates...? [Re: Liberto]
#26956368 - 09/26/20 04:14 PM (3 years, 4 months ago) |
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Quote:
Liberto said: I know you can heat up agar to make it fluid again. Then add honey and sterilize again?
i mean you could but... if you already poured the plates, & the myc develops fine without the honey, why bother?
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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