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OfflineTranscendent Other
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What defines 'Monoculture' In relation to mushrooms?
    #26645479 - 05/03/20 08:37 AM (4 years, 8 months ago)

I understand what monoculture is in broad agriculture terms, but I had difficulty finding a definition with mushroom/fungi context. For the benefits of my theory work i'd love some clarity.

Would I be right I guessing that monoculture in regards to mushroom cultivation relates to isolating a chosen phenotype of a given yield, with the aim reproducing the desired genetic characteristics of that phenotype? And thus, once i've isolated that phenotype (say I choose a mushroom for its size), I have a monoculture?

As always, thanks in advance for your wisdom!

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InvisibleStrainsfordaze


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Re: What defines 'Monoculture' In relation to mushrooms? [Re: Transcendent Other]
    #26645520 - 05/03/20 09:00 AM (4 years, 8 months ago)

A monoculture for cubes would be one dikaryotic connection. It is the isolation of an individual strain.

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OfflineKmacmo
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Re: What defines 'Monoculture' In relation to mushrooms? [Re: Strainsfordaze]
    #26645634 - 05/03/20 10:17 AM (4 years, 8 months ago)

What he said, a monoculture is a single strain (2 spores make a strain).

The only legit way to get monocultures is by using agar and transferring until you have a single set of genes on your plate, this can take a long time.
MS to agar will take the longest but if you clone then carry on transferring for a monoculture that'll save some time.

Simply choosing a mushroom you like and putting it to agar will create a clone. (clones are made up of multiple strains)


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OfflineTranscendent Other
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Re: What defines 'Monoculture' In relation to mushrooms? [Re: Kmacmo]
    #26645653 - 05/03/20 10:30 AM (4 years, 8 months ago)

Excellent, thats what I need! Thanks for your wisdom :smile:

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OfflineA.k.aM
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Re: What defines 'Monoculture' In relation to mushrooms? [Re: Transcendent Other]
    #26645838 - 05/03/20 12:06 PM (4 years, 8 months ago)

I’ve seen threads where people transfer one or two spores to a plate to do it too.

Monoculture and isolate are two of the most confusing terms used here, some use them interchangeably and some use them to mean different things.


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LAGM2020

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OfflineTranscendent Other
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Re: What defines 'Monoculture' In relation to mushrooms? [Re: A.k.a]
    #26647344 - 05/04/20 01:43 AM (4 years, 8 months ago)

Quote:

A.k.a said:
Monoculture and isolate are two of the most confusing terms used here, some use them interchangeably and some use them to mean different things.



Haha well that doesn't help! I'd imagine one isolates a strain/phenotype in a effort to create a monoculture. At least that seems to make sense to me!
I also imagine that in reality it wouldn't be possible to truly isolate a phenotype (without doing so at the microscopic level)? And what is actually achieved is more a long the lines of a culture dominated by a chosen phenotype? And the more you repeat the process of cloning, the more dominant it gets?
Just thinking out loud :smile:

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