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OfflineTrippyMomp
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Registered: 04/16/20
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mycellium on my agar ?
    #26641069 - 05/01/20 11:17 AM (3 years, 9 months ago)

does this look like the beginning of germination with spores. I have 3 diff types going. 4/26 initially inoculated 3 GT via needle and 3 EC (accidently flooded so im not sure if that's what some of the additional white discoloration from excess liquid) for viewing purposes all plates with not upside downlabeling aka sharpie on the top not bottom are from 4/26. 4/30 I inoculated 2 EC 2 GT 2 PT via spore again but loop this time. seems to have had a better effect both are producing something "crystal/snowflake appearing" I think I have afew plates not doing anything the PT were from a seller not on this site. please let me know what you think. the GT has a shiton of visible black spores and the EC looked a little brown and weird in the syringe but well see I guess that's why we do agar.

EC: 4/30-

EC: 4/26 -

GT: 4/26-

GT: 4/30-

EC: 4/30-

PT:4/30-
-no visible change that I can see.


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Offlinesporecap
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Re: mycellium on my agar ? [Re: TrippyMomp]
    #26642968 - 05/02/20 06:49 AM (3 years, 9 months ago)

In order as you posted them:

EC: 4/30: Bacterial contamination from excess liquid

EC: 4/26: Don't see anything growing, except there could be a bacterial blotch on the first one, the slight discoloration on the left. But could just be the agar itself, hard to say without better pictures.

GT: 4/26: Mycelium covered by bacteria

GT: 4/30: Bacteria from excess liquid

EC: 4/30: Bacteria from excess liquid

PT:4/30: Don't see anything growing/too blurry

Just put a single small drop of spore solution in the center of the plate next time and you'll get better results:thumbup:


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Invisiblerickyswamps
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Re: mycellium on my agar ? [Re: sporecap]
    #26642995 - 05/02/20 07:06 AM (3 years, 9 months ago)

I don't see anything on any plate.  Bacteria is cream colored than what is there.


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OfflineTrippyMomp
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Re: mycellium on my agar ? [Re: rickyswamps]
    #26643061 - 05/02/20 07:41 AM (3 years, 9 months ago)

Thanks guys. The 4/30 ones should not have gotten excess liquid unless from condensation. I'm still good to try to grow this and transfer it right ? It could also be possible that my PC didnt get hot enough. Its hottest setting is 12. I ran the aga for like 45 mins in two Mason jars. Little discouraging but wanna learn from it. You guys think I should make more plates and restart my inoculation ?


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OfflineTrippyMomp
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Re: mycellium on my agar ? [Re: rickyswamps]
    #26643322 - 05/02/20 09:44 AM (3 years, 9 months ago)

Quote:

rickyswamps said:
I don't see anything on any plate.  Bacteria is cream colored than what is there.



heres some better pics.
GT's are the only ones I think I might keep from the first inoc.
GT 4/26-

4/30 plates PT top 2 no visible growth, EC middle 2, GT bottom 2.
I know nothing but EC looks contaminated if you ask me in the first ones 4/26. GT looks promising  in both 4/26-4/30. GT and EC have similar spotted growth patterns exploding everywhere  near inoculation loop drag for 4/30 also didn't get flooded. PT im questioning the seller no visible growth  and ruined a kit I bought.


4/30- GT-


EC/4/30-


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Offlinepoisoned
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Re: mycellium on my agar ? [Re: sporecap]
    #26643406 - 05/02/20 10:32 AM (3 years, 9 months ago)

Quote:

sporecap said:
Just put a single small drop of spore solution in the center of the plate next time and you'll get better results:thumbup:





No, zig zagging with inoculation loop is way more likely to produce clean growth.


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OfflineTrippyMomp
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Re: mycellium on my agar ? [Re: poisoned]
    #26643444 - 05/02/20 10:59 AM (3 years, 9 months ago)

Quote:

poisoned said:
Quote:

sporecap said:
Just put a single small drop of spore solution in the center of the plate next time and you'll get better results:thumbup:





No, zig zagging with inoculation loop is way more likely to produce clean growth.




It's been a while since microbio but that's how you isolate colonies right it gets rid of a bunch of them along the way


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Offlinepoisoned
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Re: mycellium on my agar ? [Re: TrippyMomp]
    #26643508 - 05/02/20 11:37 AM (3 years, 9 months ago)

The main idea is that you will spread out whatever you put on agar. So, if there's some contaminants inside, they will only grow in one spot, but you will still be able to get clean growth from the other side of the plate.


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How I do glass dishes


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OfflineTrippyMomp
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Re: mycellium on my agar ? [Re: poisoned]
    #26643977 - 05/02/20 03:11 PM (3 years, 9 months ago)

Quote:

poisoned said:
The main idea is that you will spread out whatever you put on agar. So, if there's some contaminants inside, they will only grow in one spot, but you will still be able to get clean growth from the other side of the plate.




Does this look like the bacteria is from something I'm doing wrong in my sab or does it look like maybe my PC isnt hot enough. The pink thai havent done shit and the plates are still clean looking so i feel like that's almost a control example. Maybe just restart and use less spare solution ? What are your thoughts ?


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Offlinepoisoned
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Re: mycellium on my agar ? [Re: TrippyMomp]
    #26643986 - 05/02/20 03:14 PM (3 years, 9 months ago)

Some have that really clean pattern, but that could be because you swiped the contaminant that was already on the plate, it's hard to say.


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How I do glass dishes


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OfflineTrippyMomp
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Re: mycellium on my agar ? [Re: poisoned]
    #26644024 - 05/02/20 03:28 PM (3 years, 9 months ago)

Fiar enough thanks for the info I really appreciate it.  I'm gonna be using up the rest of these old plates tonight and then I'll be making new ones in glad minis. Do you recommend food coloring ?


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Offlinepoisoned
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Re: mycellium on my agar ? [Re: TrippyMomp]
    #26644074 - 05/02/20 03:56 PM (3 years, 9 months ago)

I'm too lazy and cheap for food coloring


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How I do glass dishes


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OfflineTrippyMomp
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Re: mycellium on my agar ? [Re: poisoned]
    #26644093 - 05/02/20 04:10 PM (3 years, 9 months ago)

Fair enough. Hopefuly this next batch turns out


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InvisibleRoger Clemency
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Re: mycellium on my agar ? [Re: TrippyMomp]
    #26644109 - 05/02/20 04:19 PM (3 years, 9 months ago)

The bacteria is from the spore syringe. You just have to let them all grow out a bit and see if myc pops out of the bacteria somewhere so you can transfer it. I much prefer prints for this reason


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OfflineTrippyMomp
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Re: mycellium on my agar ? [Re: Roger Clemency]
    #26644194 - 05/02/20 05:05 PM (3 years, 9 months ago)

Ok so for future reference buying and using prints to agar is easier than syringe to agar due to the fact that you lesson the amount of potential contam you have to get rid of. So you think I should just wait for now. Instead of making more plates ? I think it sounds like waiting is best. Dont really wanna waste the last 8 plates I have poured. I have 20 prepourd ones comimg in the mail just to play with and also have pda powder to make more of my own. Thinking of just buying more pda powder off the web.


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