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Clc420
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Weak clone mycelium
#26642683 - 05/02/20 02:39 AM (3 years, 8 months ago) |
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I made this transfer 6 days ago from the original clone sample. It seems quite thin compared to other photos I've seen and they myc is a lot thicker. Do you think this is worth growing out and is this normal for clones? It doesn't seem like it sectoring either which isnt always the case I guess. On the other hand my spore to agar transfer is nice a thick. Here's some photos.
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mushpunx
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Re: Weak clone mycelium [Re: Clc420]
#26642703 - 05/02/20 02:58 AM (3 years, 8 months ago) |
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when you put spores to agar, it's best to swipe the spores across the plate in a zig zag pattern across the plate with an inoculation loop.
On your clone plate, under the C looks like a little blob of bacteria and at 12 o'clock by the edge of the plate that white dog looks like a mold colony, unless these are tricks of the light.
If they are just tricks of the photo, I would grow that clone out a little more- sometimes cultures look weak until they grow out a bit. If those spots are on the plate, take another transfer before growing it out.
Sectoring can take several transfers before it becomes apparent
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Clc420
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Re: Weak clone mycelium [Re: mushpunx]
#26642723 - 05/02/20 03:15 AM (3 years, 8 months ago) |
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Quote:
mushpunx said: when you put spores to agar, it's best to swipe the spores across the plate in a zig zag pattern across the plate with an inoculation loop.
On your clone plate, under the C looks like a little blob of bacteria and at 12 o'clock by the edge of the plate that white dog looks like a mold colony, unless these are tricks of the light.
If they are just tricks of the photo, I would grow that clone out a little more- sometimes cultures look weak until they grow out a bit. If those spots are on the plate, take another transfer before growing it out.
Sectoring can take several transfers before it becomes apparent
Thanks for your input. The clone plat is a transfer from the inoculation plate. The clone plate just had a spec on top of the plastic.
The clone mycelium just looks really weak. It might thicken as it grows out more. Would you say it looks okay for 6 days or growth? It's hard to see in the pick but the transfer has a bit of ropey thich myc, was hoping that would of shot out as it grows.
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mushpunx
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Re: Weak clone mycelium [Re: Clc420]
#26642734 - 05/02/20 03:28 AM (3 years, 8 months ago) |
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Wait, you put spores to agar, and then made a transfer to another plate - is this what you are referring to as your clone plate?
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Clc420
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Re: Weak clone mycelium [Re: mushpunx]
#26642737 - 05/02/20 03:33 AM (3 years, 8 months ago) |
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Quote:
mushpunx said: Wait, you put spores to agar, and then made a transfer to another plate - is this what you are referring to as your clone plate?
No I took some spores from a print and inoculated a plate then took a transfer after it grew out a bit. I also took a clone sample from a fruit to agar and then transfered that. The plate that says gt C1 is my clone transfer and the ms plate is the ms transfer
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mushpunx
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Re: Weak clone mycelium [Re: Clc420]
#26642781 - 05/02/20 04:10 AM (3 years, 8 months ago) |
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Ahhh gotcha.
So yeah I'd grow the clone plate out a bit and see what it looks like. Sometimes I have very thin cultures that look ropey and nice by the time the plate is 2/3 of the way colonized.
The amount of nutrients in the agar can make a difference too. High nutes cause a really tomentose looking myc and low make it ropey looking
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Clc420
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Re: Weak clone mycelium [Re: mushpunx]
#26642783 - 05/02/20 04:12 AM (3 years, 8 months ago) |
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Quote:
mushpunx said: Wait, you put spores to agar, and then made a transfer to another plate - is this what you are referring to as your clone plate?
No I took some spores from a print and inoculated a plate then took a transfer after it grew out a bit. I also took a clone sample from a fruit to agar and then transfered that. The plate that says gt C1 is my clone transfer and the ms plate is the ms transferQuote:
mushpunx said: Ahhh gotcha.
So yeah I'd grow the clone plate out a bit and see what it looks like. Sometimes I have very thin cultures that look ropey and nice by the time the plate is 2/3 of the way colonized.
The amount of nutrients in the agar can make a difference too. High nutes cause a really tomentose looking myc and low make it ropey looking
Okay cool. If it doesn't thicken up would you personally still fruit it out or just start again with my spore plate ?
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mushpunx
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Re: Weak clone mycelium [Re: Clc420]
#26642792 - 05/02/20 04:19 AM (3 years, 8 months ago) |
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I might, depending on how the clone looks when I grow it out mm
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Clc420
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Re: Weak clone mycelium [Re: mushpunx]
#26642808 - 05/02/20 04:37 AM (3 years, 8 months ago) |
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Quote:
mushpunx said: I might, depending on how the clone looks when I grow it out mm
I suppose theres only one way to find out of it's worth it haha
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woofwoof
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Registered: 01/04/19
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Re: Weak clone mycelium [Re: Clc420]
#26643065 - 05/02/20 07:46 AM (3 years, 8 months ago) |
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Yea, just go for it man!
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