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Offlinekeenanosullivan
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Strange colonisation? Is this monotub fully colonised and ready to fruit?
    #26641641 - 05/01/20 04:02 PM (3 years, 8 months ago)

Hey so this is my first ever attempt to grow magic mushrooms (I'm growing cubes in this tub) and I'm not sure if whether this is fully colonised and ready to begin fruiting. I only added my colonised rye grain to my coco coir and vermiculite substrate 7 days ago. In my opinion it looks fully colonised but most post I've seen recommend at least 10-14 days as a minimum before fruiting so I don't want to jump the gun and ruin my first ever grow. Can anyone tell me whether or not to begin fruiting this tub? It has looked like this since around day 5 so I don't know if I have some super strain or whether I've done something wrong and it isn't meant to look like this hahaha.

Any help is much appreciated!



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InvisiblebodhisattaMDiscordReddit
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Re: Is this monotub fully colonized and ready to fruit? [Re: keenanosullivan]
    #26641649 - 05/01/20 04:07 PM (3 years, 8 months ago)

The entire thing is contamination. I don't there's mushrooms mycelium


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Offlinekeenanosullivan
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Re: Is this monotub fully colonized and ready to fruit? [Re: bodhisatta]
    #26641659 - 05/01/20 04:11 PM (3 years, 8 months ago)

Oh shit, really? What would it be contaminated with?


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Offlinejcm4620
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Re: Is this monotub fully colonized and ready to fruit? [Re: bodhisatta]
    #26641667 - 05/01/20 04:18 PM (3 years, 8 months ago)

ya sorry bud throw that out asap and start over. looks extremely bacterial to me. im willing to bet it was your spawn. and im also willing to bet that you shot your grains up with a spore syringe. next time use agar and make sure you have a clean culture and then put it to grain. knocking up grains with spores is risky. not that it wont work but your chances of this happening are much higher. also what was your sterile tek like??


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PANAEOLUS FRUITING MADE SIMPLE



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InvisiblebodhisattaMDiscordReddit
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Re: Is this monotub fully colonized and ready to fruit? [Re: keenanosullivan]
    #26641673 - 05/01/20 04:20 PM (3 years, 8 months ago)

Quote:

keenanosullivan said:
Oh shit, really? What would it be contaminated with?



Who knows


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Offlinekeenanosullivan
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Re: Is this monotub fully colonized and ready to fruit? [Re: jcm4620]
    #26641718 - 05/01/20 04:45 PM (3 years, 8 months ago)

So disappointing! I collected cubes in a field, took a spore print, made a spore syringe, from the syringe I inoculated some agar dishes and thought I isolated out some good mycelium which I used to inoculate my grain. Being new to it all I must have isolated bacteria thinking it was mycelium hahaha. All this time I've been doing grain to grain just growing bacteria  :facepalm3:

Back to the drawing board... thanks for your help.


Edited by keenanosullivan (05/01/20 04:52 PM)


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OfflineEnkidu
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Re: Is this monotub fully colonized and ready to fruit? [Re: keenanosullivan]
    #26641741 - 05/01/20 04:54 PM (3 years, 8 months ago)

Damn dude that sucks


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Offlinejcm4620
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Re: Is this monotub fully colonized and ready to fruit? [Re: Enkidu]
    #26641756 - 05/01/20 05:02 PM (3 years, 8 months ago)

well at least ur using agar๐Ÿ‘๐Ÿ‘ hang in there bro and try again. you will get there man dont worry.


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PANAEOLUS FRUITING MADE SIMPLE



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OfflineCylarabes
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Re: Is this monotub fully colonized and ready to fruit? [Re: Enkidu]
    #26641760 - 05/01/20 05:03 PM (3 years, 8 months ago)

I feel for ya buddy



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Offlinekeenanosullivan
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Re: Is this monotub fully colonized and ready to fruit? [Re: jcm4620]
    #26641924 - 05/01/20 06:19 PM (3 years, 8 months ago)

This is the agar dish I used to inoculate my grain. Are you able to tell from these pictures whether it's mycelium or bacteria?


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Offlinejcm4620
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Re: Is this monotub fully colonized and ready to fruit? [Re: keenanosullivan]
    #26641936 - 05/01/20 06:24 PM (3 years, 8 months ago)

prolly both i never let plates grow that close to the outer edges


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Offlinekeenanosullivan
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Re: Is this monotub fully colonized and ready to fruit? [Re: jcm4620]
    #26641945 - 05/01/20 06:28 PM (3 years, 8 months ago)

Ok thanks a heap for your help, much appreciated.


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Offlinepoisoned
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Re: Is this monotub fully colonized and ready to fruit? [Re: keenanosullivan]
    #26642800 - 05/02/20 04:32 AM (3 years, 8 months ago)

Looks like myc is fighting something, do a few more transfers. That growth at 7 and 9 o'clock is suspicious.

Plates growing to the outer edges is not an issue tho.


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How I do glass dishes


Edited by poisoned (05/02/20 04:33 AM)


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OfflineDrizzleDrazzle
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Re: Is this monotub fully colonized and ready to fruit? [Re: poisoned]
    #26642887 - 05/02/20 05:59 AM (3 years, 8 months ago)

definatley looks like that dish was fighting something off on the left side there, sounds like you did everything right just bad luck :/ just keep trying itll work out eventually :smile:


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