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MuckPeddler
Stranger

Registered: 01/31/20
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Question about PF tek!
#26640293 - 05/01/20 01:15 AM (3 years, 9 months ago) |
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Heya! So I am attempting PF tek, and unfortunately my last jars were contaminated with bacteria. I was extra careful when sterilizing this time but I was wondering if I am able to store the jars in a container while they colonize? Or will keeping them in a closed tub not work?
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HamHead
Hard Ass Motherfucker



Registered: 03/17/15
Posts: 6,107
Loc: Galactic sector ZZ9 Plura...
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Re: Question about PF tek! [Re: MuckPeddler] 1
#26640487 - 05/01/20 04:47 AM (3 years, 9 months ago) |
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Light is beneficial for mycelium during all stages of growth.
Leave them out to get a little light. They will colonize in darkness but better in some light.
-------------------- The Italian researchers’ findings, published by the INT’s scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February. https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF This online first version has been peer-reviewed, accepted and edited, but not formatted and finalized with corrections from authors and proofreaders https://www.icandecide.org/
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Question about PF tek! [Re: HamHead] 2
#26640530 - 05/01/20 05:31 AM (3 years, 9 months ago) |
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If you got bacteria, unless you really screwed up sterilizing your jars it's usually either from the syringe or a mistake during inoculation.
The most common mistake is people wiping the needle off with alcohol after flame sterilization
--------------------
 Amateur Mycologists United AMU Q&A
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MuckPeddler
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Registered: 01/31/20
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Re: Question about PF tek! [Re: mushpunx]
#26641427 - 05/01/20 02:08 PM (3 years, 9 months ago) |
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Oh! Ya I did wipe my syringe off so that probably did it Lol thanks for the help
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Ive seen photos of people making their jar mixtures too wet, causing bacteria. What does your PF material look like thru the glass around the jars? Can you provide a photo?
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MuckPeddler
Stranger

Registered: 01/31/20
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Last seen: 4 months, 23 days
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So here is a picture of the jar from last time, I tried to get it so the substrate was dampish but maybe it is too wet? I made 2 new jars and these ones had bacteria too :-( Should I just see if they keep growing or is it best to get rid of them? Its a bummer lol but Ill try again tomorrow
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,030
Loc: Temple of Time
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let it ride a few more days. if that pocket gets bigger toss it. what have you got to lose really?
could be nothing, could be bacteria.
-------------------- Song Of Healing
Updated Pf Tek Guide Ziran's Teks AMU Q&A Thread The Chinese word for nature is zìrán and it means that of which is of itself.

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