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InvisibleAntigov
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Question about grain to grain transfer
    #26641074 - 05/01/20 11:20 AM (3 years, 8 months ago)

Just before I start, I did search my question first and I didn’t come up with anything, so here it goes. Around end December, or first of January I cloned a really nice PE specimen, I did a agar to grain transfer and from there I have been doing grain to grain transfer from it since. My question is how long can I do g2g transfers on this batch before there are any issues. Any insight would be greatly appreciated.


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OfflineEnkidu
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Re: Question about grain to grain transfer [Re: Antigov] * 2
    #26641725 - 05/01/20 04:49 PM (3 years, 8 months ago)

Ive done it like 4 times i think. Maybe more

Make more agar and use agar

You still got the original on plates?


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OfflineHide
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Re: Question about grain to grain transfer [Re: Enkidu] * 1
    #26641758 - 05/01/20 05:03 PM (3 years, 8 months ago)



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Offlineverum subsequentis
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Re: Question about grain to grain transfer [Re: Hide] * 2
    #26641932 - 05/01/20 06:22 PM (3 years, 8 months ago)

Most dunt go further than three or four. There isn't really a scientifically verified answer to you question because it entirely depends on how much grain/ myc is being transferred to each bag/ jar. If you stretched 1 jar to two new jars the cell division would be way less than stretching one jar to 18 4 quart bags (yes, you can do that).

Another reason is that most don't push the limits because every generation is further and further from your verifiably clean culture. Meaning, the more generations deep you get the more vectors you've had.

Also, if you follow the good old advice, 1 becomes 10... These 10 become 100... Those 100 become 1000. So, your three generations in and have a thousand jars. Obviously you don't need more than that.


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InvisibleAntigov
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Re: Question about grain to grain transfer [Re: Enkidu]
    #26642118 - 05/01/20 08:13 PM (3 years, 8 months ago)

I don’t have the original plates, I’m running low on alcohol so I figure g2g transfer would save on supplies.


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InvisibleAntigov
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Re: Question about grain to grain transfer [Re: Hide]
    #26642123 - 05/01/20 08:14 PM (3 years, 8 months ago)

Thank you for the links


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InvisibleAntigov
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Re: Question about grain to grain transfer [Re: verum subsequentis]
    #26642139 - 05/01/20 08:23 PM (3 years, 8 months ago)

Quote:

verum subsequentis said:
Most dunt go further than three or four. There isn't really a scientifically verified answer to you question because it entirely depends on how much grain/ myc is being transferred to each bag/ jar. If you stretched 1 jar to two new jars the cell division would be way less than stretching one jar to 18 4 quart bags (yes, you can do that).

Another reason is that most don't push the limits because every generation is further and further from your verifiably clean culture. Meaning, the more generations deep you get the more vectors you've had.

Also, if you follow the good old advice, 1 becomes 10... These 10 become 100... Those 100 become 1000. So, your three generations in and have a thousand jars. Obviously you don't need more than that.




Gotcha. Im probably at 5 with this batch. It may be my imagination but I have noticed that this recent patch doesn’t have the vigor the last generation did. As some as I can get my hands on some alcohol I’m going to start some new plates.


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Offlineverum subsequentis
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Re: Question about grain to grain transfer [Re: Antigov]
    #26643366 - 05/02/20 10:12 AM (3 years, 8 months ago)

How do plates require less ISO than G2G? The same exact principles are in play.


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Offlinepoisoned
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Re: Question about grain to grain transfer [Re: verum subsequentis]
    #26643413 - 05/02/20 10:38 AM (3 years, 8 months ago)

G2G requires less ISO/amount of inoculate I guess.


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OfflineLogicaL ChaosM
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Re: Question about grain to grain transfer [Re: Antigov]
    #26643566 - 05/02/20 12:09 PM (3 years, 8 months ago)

I actually asked the same question about 11 years ago. Crazy right? Little personal Shroomery History.

https://www.shroomery.org/forums/showflat.php/Number/10154262#10154262


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