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OfflineMuckPeddler
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Registered: 01/31/20
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Question about PF tek!
    #26640293 - 05/01/20 01:15 AM (3 years, 9 months ago)

Heya! So I am attempting PF tek, and unfortunately my last jars were contaminated with bacteria. I was extra careful when sterilizing this time but I was wondering if I am able to store the jars in a container while they colonize? Or will keeping them in a closed tub not work?


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OfflineHamHead
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Re: Question about PF tek! [Re: MuckPeddler] * 1
    #26640487 - 05/01/20 04:47 AM (3 years, 9 months ago)

Light is beneficial for mycelium during all stages of growth.

Leave them out to get a little light. They will colonize in darkness but better in some light.


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The Italian researchers’ findings, published by the INT’s scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February.

https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF

This online first version has been peer-reviewed, accepted and edited,  but not formatted and finalized with corrections from authors and proofreaders

https://www.icandecide.org/


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Offlinemushpunx
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Re: Question about PF tek! [Re: HamHead] * 2
    #26640530 - 05/01/20 05:31 AM (3 years, 9 months ago)

If you got bacteria, unless you really screwed up sterilizing your jars it's usually either from the syringe or a mistake during inoculation.

The most common mistake is people wiping the needle off with alcohol after flame sterilization


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AMU Q&A


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OfflineMuckPeddler
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Re: Question about PF tek! [Re: mushpunx]
    #26641427 - 05/01/20 02:08 PM (3 years, 9 months ago)

Oh! Ya I did wipe my syringe off so that probably did it :tongue: Lol thanks for the help


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OfflineLogicaL ChaosM
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Re: Question about PF tek! [Re: MuckPeddler]
    #26641447 - 05/01/20 02:26 PM (3 years, 9 months ago)

Ive seen photos of people making their jar mixtures too wet, causing bacteria. What does your PF material look like thru the glass around the jars? Can you provide a photo?


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OfflineMuckPeddler
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Re: Question about PF tek! [Re: LogicaL Chaos]
    #26642298 - 05/01/20 10:07 PM (3 years, 9 months ago)



So here is a picture of the jar from last time, I tried to get it so the substrate was dampish but maybe it is too wet? I made 2 new jars and these ones had bacteria too :-( Should I just see if they keep growing or is it best to get rid of them? Its a bummer lol but Ill try again tomorrow


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InvisibleZiran
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Re: Question about PF tek! [Re: MuckPeddler]
    #26642542 - 05/02/20 01:10 AM (3 years, 9 months ago)

let it ride a few more days. if that pocket gets bigger toss it. what have you got to lose really?

could be nothing, could be bacteria.


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Ziran's Teks
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The Chinese word for nature is zìrán and it means that of which is of itself.



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