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Offlinepattycakes77
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Contaminated Jars? Need your input! * 1
    #26638893 - 04/30/20 12:08 PM (3 years, 8 months ago)

So I'm a first time grower and I am a little concerned on what I'm seeing in my BRF cakes.  I inoculated my jars a week ago today with the Brazil corumba strain and I have been noticing a difference in color and texture on what my mycelium looks like, compared to what I have seen online. The color is more grey around the edges of where the mycelium is growing. And some areas are just grey ish color, no white. I did PC sterilization on my jars.  Just would like to get someone's input who's experienced.  I wasn't sure if it is contaminated that it might be cobweb or sour rot?  I read that those two are the more common.  What do you think?  Any feedback would be appreciated!!



Update:
Two week mark for colonization.  Overall still looks the same.  Its weird how some spots are more of a grey color and some have the white mycelium spots in it.  Still not sure 100% if its contaminated.  Can anyone tell me what that crystallization-like substance between the cake and jar is?  Not sure if that's normal or not.



Edited by pattycakes77 (05/07/20 05:54 PM)


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OfflineEnkidu
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Re: Contaminated Jars? Need your input! [Re: pattycakes77]
    #26638964 - 04/30/20 01:03 PM (3 years, 8 months ago)

Looks off to me but weird all of them would look the same

Was your brf mixed correctly and brought to field capacity?

You interested in working with agar?

If i was doing only brf jars i would nocc up as many as i had enough spores for


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Offlinepattycakes77
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Re: Contaminated Jars? Need your input! [Re: Enkidu]
    #26639102 - 04/30/20 01:59 PM (3 years, 8 months ago)

Yep brf was 2to 1 ratio verm to brown rice flour and water.  That's what I pretty much did as well.  I mixed enough to do the whole 10cc syringe, so I have 10 jars and they all pretty much look the same.  Cleaned the lip of the jars on the inside and put dry vermiculite on top before sterilization.  I haven't worked with agar before or looked into it quite yet.  But if it helps with better results then I'm all for it.  I'm having g lots of fun with my new hobby so far :smile:  Anyways, I'm thinking of just leaving it to see what happens, and in the mean time I might start another batch (golden teacher) just in case my corumba fails to produce.


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InvisibleRoger Clemency
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Re: Contaminated Jars? Need your input! [Re: pattycakes77]
    #26639111 - 04/30/20 02:02 PM (3 years, 8 months ago)

I don’t know if the tape over the holes is causing the strange look but it can hamper colonization. The dry verm barrier is sufficient protection, as long as you don’t shake the jars around


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OfflineEnkidu
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Re: Contaminated Jars? Need your input! [Re: pattycakes77]
    #26639114 - 04/30/20 02:04 PM (3 years, 8 months ago)

I would.

Those all your jars?

Agar is easy

https://www.shroomery.org/forums/showflat.php/Number/23890579

Mix ingredients, pc, let cool, pour plates in SAB, inoc plates in SAB

Then you can grow a lot of mushrooms if you want

Agar is fun once you get a grow and want to clone


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Offlinemushpunx
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Re: Contaminated Jars? Need your input! [Re: Enkidu]
    #26639119 - 04/30/20 02:05 PM (3 years, 8 months ago)

It's hard to tell from the photos but the color and textures look wrong. It looks purple in places to me

When you inoculated, did you flame sterilize the needle before each inoculation? You didn't wipe the needle with alcohol or anything after flame sterilizing?


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Offlinepattycakes77
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Re: Contaminated Jars? Need your input! [Re: mushpunx]
    #26639162 - 04/30/20 02:21 PM (3 years, 8 months ago)

Nope didn't wipe with alcohol.  I did flame sterilization before each jar.  I'm wondering if I should take the tape off to see what happens moving forward, but I doubt it's going to change anything.  And yea, it does look like a slight purple color on some spots.


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Offlinepattycakes77
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Re: Contaminated Jars? Need your input! [Re: Enkidu]
    #26639169 - 04/30/20 02:24 PM (3 years, 8 months ago)

It looks interesting.  Lime a fool proof way to get clean mycelium with out contaminating.  I have 24 jars in total. So far 10 are inoculated


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Re: Contaminated Jars? Need your input! [Re: pattycakes77]
    #26639197 - 04/30/20 02:35 PM (3 years, 8 months ago)

Since you inoculated well, it's most likely your syringe that is contaminated.

Spore syringes are never %100 clean, that's why we don't use them on grains- but they are usually fine for BRF Cakes.

But contaminated syringes happen. Do you have other syringes?


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Offlinepattycakes77
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Re: Contaminated Jars? Need your input! [Re: mushpunx] * 1
    #26639214 - 04/30/20 02:42 PM (3 years, 8 months ago)

Yea.  Got a golden teacher and a Cambodian strain.  Also got 2 wavy caps syringe, but that's for my outdoor grow later.


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OfflineEnkidu
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Re: Contaminated Jars? Need your input! [Re: pattycakes77]
    #26639318 - 04/30/20 03:29 PM (3 years, 8 months ago)

Wavy caps?

Nice.. Sounds fucking sweet.

I would really love not to miss a thread on that if you do a grow log for them

Id love to read that


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OfflineLogicaL ChaosM
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Re: Contaminated Jars? Need your input! [Re: pattycakes77]
    #26639335 - 04/30/20 03:36 PM (3 years, 8 months ago)

Thats one week?! Damn! U got a good vendor :eek:

They look fine to me. Sometimes mycelium will look kinda greyish :shrug:

Id just wait and let them colonize more.


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Offlinepattycakes77
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Re: Contaminated Jars? Need your input! [Re: LogicaL Chaos] * 1
    #26639403 - 04/30/20 04:16 PM (3 years, 8 months ago)

Yea somebody else mentioned they looked fine to him too.  I'm going to hang on to them to see what happens.  In the mean time I've already have cakes PC'in for my next batch just in case :smile:  exciting times!


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OfflineLogicaL ChaosM
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Re: Contaminated Jars? Need your input! [Re: pattycakes77]
    #26639416 - 04/30/20 04:23 PM (3 years, 8 months ago)

Nice! Always a good idea to look ahead to the next project. Keep the cycle flowin' :cool:


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Offlinemushpunx
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Re: Contaminated Jars? Need your input! [Re: pattycakes77] * 1
    #26639436 - 04/30/20 04:35 PM (3 years, 8 months ago)

Quote:

pattycakes77 said:
Yea.  Got a golden teacher and a Cambodian strain.  Also got 2 wavy caps syringe, but that's for my outdoor grow later.





Good! Start new cakes with different syringe. Save some to get into agar work too!


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