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OfflineThe Mycologist
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Re: Agar from MS syringe question [Re: tripdawg420] * 1
    #26638675 - 04/30/20 10:09 AM (3 years, 8 months ago)

I have had both molds and bacteria riding along with a culture.

They dont always act like there is a barrier.

Streaking comes from actual laboratory practice, you think they do it for no reason?


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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineNightPuma1
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Re: Agar from MS syringe question [Re: The Mycologist]
    #26640028 - 04/30/20 09:54 PM (3 years, 8 months ago)

No, it just seems to me that if you have 2 methods of spreading out a drop and one of them does not involve sticking something in there then that was the way to go. Apparently I am mistaken - I just haven't really gotten a great answer of why exactly that is.  Why a zig-zag pattern is better than the streak I get by letting it slide. I believe you I just don't understand.


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OfflineSYF8
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Re: Agar from MS syringe question [Re: NightPuma1]
    #26640052 - 04/30/20 10:11 PM (3 years, 8 months ago)

How about a 3rd option, put a couple drops on a sterile swab and streak with that.


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InvisibleGrenade01
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Re: Agar from MS syringe question *DELETED* [Re: SYF8] * 1
    #26640197 - 05/01/20 12:13 AM (3 years, 8 months ago)

Post deleted by Grenade01

Reason for deletion: .


Edited by Grenade01 (05/01/20 12:14 AM)


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OfflineLogicaL ChaosM
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Re: Agar from MS syringe question [Re: Grenade01]
    #26640205 - 05/01/20 12:19 AM (3 years, 8 months ago)

I hate when i shoot too much spores :awehigh:


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OfflineMrMong


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Re: Agar from MS syringe question [Re: LogicaL Chaos]
    #26640252 - 05/01/20 12:44 AM (3 years, 8 months ago)

How would introducing too many spores causes issues?


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OfflineLogicaL ChaosM
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Re: Agar from MS syringe question [Re: MrMong]
    #26640261 - 05/01/20 12:50 AM (3 years, 8 months ago)

It makes it harder to seperate good mycelium from contaminates. Since its all microscopic, it will make it a pain in the ass to cut sections off. Thats why streaking is preferred because it spreads the spores over a wider area in the agar, thus making sectoring easier.


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Offlinepoisoned
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Re: Agar from MS syringe question [Re: NightPuma1]
    #26640296 - 05/01/20 01:17 AM (3 years, 8 months ago)

Quote:

NightPuma1 said:
No, it just seems to me that if you have 2 methods of spreading out a drop and one of them does not involve sticking something in there then that was the way to go. Apparently I am mistaken - I just haven't really gotten a great answer of why exactly that is.  Why a zig-zag pattern is better than the streak I get by letting it slide. I believe you I just don't understand.




You flame sterilize that thing, it shouldn't be an issue at all.


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Offlinemushpunx
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Re: Agar from MS syringe question [Re: NightPuma1] * 1
    #26640524 - 05/01/20 05:27 AM (3 years, 8 months ago)

Prints are never 100% clean, and therefore spore syringes are not either.
Unless you have sloppy technique, contamination on the plate is a lot more likely to grow from the spore solution that be introduced by a sterilized loop.

When you let that spore solution slide around the plate, any potential mold colonies (which are often white and resemble mushroom myc) and bacteria (which can be blobs- or a light film that is very hard to spot) will grow in the same area as the germination, they might touch or the myc might grow over or into it. It doesn't mean you won't be able to transfer some clean myc away from it, but when you swipe the spores in a zig zag pattern across the plate, first you know that the germination should occur in that pattern and not to transfer from anything outside of the zig zag, and second it spreads the spores out so that you might more easily be able to take a transfer of clean myc that is far away from mold or bacteria.

You don't have to do this, it's just good technique. If you are going to be working with agar you are going to have to learn to use sterile tools


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Edited by mushpunx (05/01/20 05:28 AM)


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OfflineThe Mycologist
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Re: Agar from MS syringe question [Re: NightPuma1]
    #26640605 - 05/01/20 06:40 AM (3 years, 8 months ago)

Quote:

NightPuma1 said:
No, it just seems to me that if you have 2 methods of spreading out a drop and one of them does not involve sticking something in there then that was the way to go. Apparently I am mistaken - I just haven't really gotten a great answer of why exactly that is.  Why a zig-zag pattern is better than the streak I get by letting it slide. I believe you I just don't understand.




You have to have faith in the techniques.

This whole thing relies on flame sterilization working how its intended.

But If you want to limit your vectors, that has logic too, and may be good for someone starting out.

...

I was originally responding to the guy who was saying that contams cant grow along with mycelium.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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