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OfflineNightPuma1
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Agar from MS syringe question
    #26637162 - 04/29/20 04:24 PM (3 years, 8 months ago)

I would like sort of a vote if possible on the preferred method BETWEEN THESE TWO (I know a lot of people do streaking). Using a MS syringe on agar:

1. 3 drops on the petri dish in a triangular pattern

2. 1 drop in the center, followed by tilting the dish slightly causing the drop to run towards the edge (but not touching it).

Thank you!


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OfflineHouseOfRoses
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Re: Agar from MS syringe question [Re: NightPuma1] * 1
    #26637168 - 04/29/20 04:25 PM (3 years, 8 months ago)

I havent actually used agar with an MS syringe yet, but I was always told just one drip in the middle and no tilting the dish


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Re: Agar from MS syringe question [Re: HouseOfRoses] * 1
    #26637171 - 04/29/20 04:28 PM (3 years, 8 months ago)

#2 if I had to choose between the 2 :shrug:


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OfflineNightPuma1
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Re: Agar from MS syringe question [Re: Tormato]
    #26637373 - 04/29/20 06:16 PM (3 years, 8 months ago)

If I may ask, what is the actual advantage in introducing a new potential contamination vector (meaning the inoculation loop) just to get the droplet to do a zig-zag pattern?


Edited by NightPuma1 (04/29/20 06:17 PM)


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Re: Agar from MS syringe question [Re: NightPuma1] * 3
    #26637378 - 04/29/20 06:19 PM (3 years, 8 months ago)

To spread it out which won't be a vector if you flame sterilize the loop first.

Honestly the MS syringe is gonna be your biggest potential vector


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Re: Agar from MS syringe question [Re: Tormato] * 2
    #26637466 - 04/29/20 07:02 PM (3 years, 8 months ago)

:whathesaid:

When you streak the spores you should see germination in the zig zag pattern of the streak and have a much easier time distinguishing clean germination from things like mold colonies + bacteria.

If you are going to be working with agar, you are going to have to get used to opening the lid and using tools like inoculation loops and scalpels to swipe and make transfers. 


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Edited by mushpunx (04/30/20 02:42 AM)


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OfflineLogicaL ChaosM
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Re: Agar from MS syringe question [Re: NightPuma1]
    #26637761 - 04/29/20 09:56 PM (3 years, 8 months ago)

I vote one drip in the center, no tilting. Dont forgot to shake the syringe first !


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Re: Agar from MS syringe question [Re: LogicaL Chaos] * 1
    #26637828 - 04/29/20 10:39 PM (3 years, 8 months ago)

Quote:

LogicaL Chaos said:
I vote one drip in the center, no tilting. Dont forgot to shake the syringe first !



:thumbup:


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OfflineLogicaL ChaosM
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Re: Agar from MS syringe question [Re: tripdawg420]
    #26637835 - 04/29/20 10:43 PM (3 years, 8 months ago)

:awebig:


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Offlinemistermushly

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Re: Agar from MS syringe question [Re: LogicaL Chaos] * 1
    #26637847 - 04/29/20 10:53 PM (3 years, 8 months ago)

Assuming it's a well shaken syringe, as little as possible, one drop in the center with as little liquid as possible seems the best to me. just my 2¢


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Offlinepoisoned
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Re: Agar from MS syringe question [Re: mistermushly]
    #26638050 - 04/30/20 01:14 AM (3 years, 8 months ago)

I used to do a drop in the center. Streaking is 10x better.


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Re: Agar from MS syringe question [Re: poisoned] * 2
    #26638298 - 04/30/20 05:25 AM (3 years, 8 months ago)

The draw back to leaving the drop in the center is that now everything is going to grow in a big mass ..contams included.

Always best to spread it out for visibility reasons.


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OfflineLogicaL ChaosM
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Re: Agar from MS syringe question [Re: Tormato]
    #26638369 - 04/30/20 06:44 AM (3 years, 8 months ago)

Can u streak with a syringe needle or do u need a Inocu loop?


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Re: Agar from MS syringe question [Re: LogicaL Chaos] * 1
    #26638444 - 04/30/20 07:33 AM (3 years, 8 months ago)

Quote:

LogicaL Chaos said:
Can u streak with a syringe needle or do u need a Inocu loop?



You can, but I find a loop just spreads it better


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Offlinepoisoned
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Re: Agar from MS syringe question [Re: Tormato] * 1
    #26638453 - 04/30/20 07:38 AM (3 years, 8 months ago)

I tried to, but it's a bit more tricky. You can make a loop from materials you probably have at home in a minute.


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Offlinetripdawg420
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Re: Agar from MS syringe question [Re: Tormato] * 1
    #26638552 - 04/30/20 08:40 AM (3 years, 8 months ago)

Quote:

Tormato said:
The draw back to leaving the drop in the center is that now everything is going to grow in a big mass ..contams included.

Always best to spread it out for visibility reasons.



contams and myc wont mix together:shrug:


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coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflineThe Mycologist
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Re: Agar from MS syringe question [Re: tripdawg420]
    #26638569 - 04/30/20 08:53 AM (3 years, 8 months ago)

Quote:

tripdawg420 said:
Quote:

Tormato said:
The draw back to leaving the drop in the center is that now everything is going to grow in a big mass ..contams included.

Always best to spread it out for visibility reasons.



contams and myc wont mix together:shrug:




Yes they can


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Offlinetripdawg420
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Re: Agar from MS syringe question [Re: The Mycologist]
    #26638573 - 04/30/20 08:55 AM (3 years, 8 months ago)

Quote:

The Mycologist said:
Quote:

tripdawg420 said:
Quote:

Tormato said:
The draw back to leaving the drop in the center is that now everything is going to grow in a big mass ..contams included.

Always best to spread it out for visibility reasons.



contams and myc wont mix together:shrug:




Yes they can



oh ya plz explain


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epic GT mono tub
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http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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Offlinemushpunx
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Re: Agar from MS syringe question [Re: tripdawg420] * 1
    #26638660 - 04/30/20 10:01 AM (3 years, 8 months ago)

You definitely want to take transfers of myc that haven't grown into/over or collided with bacteria/mold colonies.

I have had bacterial cultures before , they get this strange puffy look (not tomentose). Man it's hard to explain I wish I still had some photos


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Re: Agar from MS syringe question [Re: mushpunx] * 1
    #26638674 - 04/30/20 10:08 AM (3 years, 8 months ago)

:whathesaid: Right...it's just easier to discern what's what and what hasn't been possibly compromised when you spread things out :shrug:


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