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OlympusMyco.com Shop: We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)

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OfflineClc420
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How to get perfectly clear agar?
    #26637009 - 04/29/20 02:56 PM (4 years, 8 months ago)

On using agar and light malt extract with food colouring. It's not coming out clear, I follow bods agar tek and his is clear. If i use extra light malt extract would it come out more clear ?

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OfflineGrowtech
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Re: How to get perfectly clear agar? [Re: Clc420]
    #26637034 - 04/29/20 03:12 PM (4 years, 8 months ago)

Well nothing is going to be crystal clear like glass... but yes, the lighter the nutrient media you use, the clearer your end result will be.  Also, adding food coloring is going to be counter-productive if your goal is "perfectly clear".


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OfflineCrackatoa
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Re: How to get perfectly clear agar? [Re: Growtech] * 1
    #26637043 - 04/29/20 03:17 PM (4 years, 8 months ago)

1% LME makes them pretty clear. I hear my water and stir thoroughly until it's all broken up before I fill my media bottle and PC.


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InvisibleCaps McGee
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Re: How to get perfectly clear agar? [Re: Crackatoa]
    #26637052 - 04/29/20 03:23 PM (4 years, 8 months ago)

I'm at 0.8% agar and 1.3% LME... mix using cold water, and do so quickly after adding LME to prevent clumps... pour plates around 117°F just warm enough to remain liquid and reduce degree of rapid cooling, in effect the condensation as well...

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Invisiblegizmo1
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Re: How to get perfectly clear agar? [Re: Caps McGee]
    #26637152 - 04/29/20 04:20 PM (4 years, 8 months ago)

If you mix cold and don't heat until you pressure cook the agar you have less chance of overcooking and getting those little burned protein shits in our agar. Idk if that's one of the problems your having or not.
If your shits just coming out cloudy try using distilled water or filtering before pcing. Or both.
If you still get plates that aren't clear after making these changes then you are pouring too thick. This actually makes a big difference as to rather your plates look clear or not.


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OlympusMyco.com Shop: We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)


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