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OfflineJbrady5555
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Registered: 12/10/19
Posts: 124
Last seen: 1 year, 1 month
First transfer pics, help me understand
    #26636877 - 04/29/20 01:39 PM (4 years, 8 months ago)

So I did my first transfer today on my 12 day old agar plates. I took several pics to post here so that someone can help me understand what kind of growth I’m looking at and whether I’m cutting em wedges out of the correct places. Thanks for any info.




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Invisiblemushhead
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I'm a teapot


Registered: 08/22/14
Posts: 2,242
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Re: First transfer pics, help me understand [Re: Jbrady5555]
    #26636901 - 04/29/20 01:49 PM (4 years, 8 months ago)

okay so I see a few points of contamination in the first pic to the right side of your dish, second pic is tomentose growth and I see no contamination, third pic I see your myc in the middle but also contams to the left and right, fourth pic looks a little messy but no contams I can see, the fifth pic is BEAUTIFUL from where I see you dropped spore in two spots which gave off rhizomorphic growth but turned tomentose when all the myc came together.

Your wedges look nicely cut, and it doesn't matter where you take cuts from - taking cuts is the main way of isolating genetics and assist in creating plates that look like art with round rhizomorphic growth.

You can see here in this pic i've taken cuts of the outwardly growth and took no spores with it - essentially isolating those genetics from the rest of the tomentose growth and placing them on their own plate which grew into this

You can tell that the size, shape, and placement of my transfer wedges is not defined by really anything except by how lazy I am being.
You'll get the hang of it OP!  https://www.shroomery.org/forums/showflat.php/Number/26604440/page/1


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Edited by mushhead (04/29/20 02:05 PM)

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OfflineJbrady5555
Stranger
Registered: 12/10/19
Posts: 124
Last seen: 1 year, 1 month
Re: First transfer pics, help me understand [Re: mushhead]
    #26636958 - 04/29/20 02:20 PM (4 years, 8 months ago)

Quote:

mushhead said:
okay so I see a few points of contamination in the first pic to the right side of your dish, second pic is tomentose growth and I see no contamination, third pic I see your myc in the middle but also contams to the left and right, fourth pic looks a little messy but no contams I can see, the fifth pic is BEAUTIFUL from where I see you dropped spore in two spots which gave off rhizomorphic growth but turned tomentose when all the myc came together.

Your wedges look nicely cut, and it doesn't matter where you take cuts from - taking cuts is the main way of isolating genetics and assist in creating plates that look like art with round rhizomorphic growth.

You can see here in this pic i've taken cuts of the outwardly growth and took no spores with it - essentially isolating those genetics from the rest of the tomentose growth and placing them on their own plate which grew into this

You can tell that the size, shape, and placement of my transfer wedges is not defined by really anything except by how lazy I am being.
You'll get the hang of it OP!  https://www.shroomery.org/forums/showflat.php/Number/26604440/page/1





Wow! Thanks man that was great info. I’m still not quite grasping how to spot contams, it all looks like the same white stuff to me lol. I really appreciate you going through my plates like that.

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