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Offlinerumfor69
Bodhicitta Cultivator
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Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
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Re: PE spore syringe to agar....can anyone confirm? [Re: UmbraSprite]
    #26636604 - 04/29/20 11:19 AM (3 years, 9 months ago)

I'm thinking all of those look like clean healthy mycelium. The fuzzier
variety called tomentose, but healthy. If it was me I'd do some transfers
from all of those selecting the more aggressive ropy samples and I'd make
some grain jars to toss some into for experimentation. The goal here being
to continue to propagate healthy mycelium and to get something that will
get you some fruit so you can make a clone to agar.


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OfflineUmbraSprite
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Registered: 08/29/19
Posts: 36
Last seen: 3 years, 4 months
Re: PE spore syringe to agar....can anyone confirm? [Re: rumfor69]
    #26637582 - 04/29/20 08:11 PM (3 years, 9 months ago)

Yeah that B+ is cloned from fruit.  I always keep the last two plates so I have backup.

The first batch of agar I bought was very transparent.  The next few I can see particulate in it.  I wonder if the different agar are causing different growth morphology?

I have been using 1/2 pint BRF spawn jars.  As I try this PE I have been seeing a lot of people use other substrate as well as casing.  Anyone have thoughts on differences in spawn substrate and anything helpful when moving from B+ to PE?


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OfflineUmbraSprite
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Registered: 08/29/19
Posts: 36
Last seen: 3 years, 4 months
Re: PE spore syringe to agar....can anyone confirm? [Re: UmbraSprite]
    #26641502 - 05/01/20 02:55 PM (3 years, 9 months ago)

This has me thinking even that original plate may have been mycelium.  I have just never had it grow so "fuzzy" and so far up vertically that it "smushes" against the plate lid.  I am fairly certain had it not been taped it would have pushed the lid right off.


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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
Last seen: 1 hour, 4 minutes
Re: PE spore syringe to agar....can anyone confirm? [Re: UmbraSprite]
    #26642352 - 05/01/20 10:50 PM (3 years, 9 months ago)

They say a nutrient rich agar will cause that tomentose(fuzzy) mycelium
to form more. If you plate is a little on the less nutritious side is
helps with the growth of rhizomorph(ropy) mycelium.


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