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Offlinespyrelx
Stranger

Registered: 05/09/10
Posts: 51
Last seen: 1 year, 2 months
Quick green mold question
    #26636542 - 04/29/20 10:52 AM (3 years, 8 months ago)

I've colonized 12 jars, using two syringes of different strains.  All done at the same time with the same method, utilizing a poor man's glove box and sterilizing the needle in between jars.

It looks like one jar developed a hefty case of green mold.  All others look healthy white (see pics).  I've never had mold in a jar before, wo I have a few somewhat paranoid questions.

First question:  It's weird to me that one jar developed mold but others show no trace.  I.e., why would only one get it when procedures were all the same?  Is this just the way it goes sometimes?

Second question:  I've removed the green jar from the others and will destroy it.  I'm going to wait another few days/week before birthing the others.  If they show no signs of mold on birthing, then it should be ok to put them all in my SGFC, right?

Thanks for your help.



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OfflineBroken Veils
Something

Registered: 04/26/20
Posts: 41
Loc: Somewhere
Last seen: 3 years, 26 days
Re: Quick green mold question [Re: spyrelx]
    #26636705 - 04/29/20 12:00 PM (3 years, 8 months ago)

How did you sterilize the needle between jars?  Flame sterilizing is the way to go - avoid alcohol wipes...constantly evaluate and think through your sterile technique :syringe: We all have moments where contams sneak in :mad2:

The other jars should be fine if they look like your 2nd pic. When birthed they should come with a earthy mushroom smell...anything with an off putting or sour smell should be tossed.


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OfflineDoc9151M
Mycologist
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Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA Flag
Last seen: 1 year, 6 months
Trusted Cultivator
Re: Quick green mold question [Re: Broken Veils]
    #26636711 - 04/29/20 12:03 PM (3 years, 8 months ago)

syringes are inherently dirty,  agar is simple and easy, it allows you to have clean cultures and avoid contamination.


Edit: don't open that jar in your house or it will blow spores everywhere!!! I fill a sink or bucket with water and dawn dish soap then submerge the jar and then open under water flooding the jar and then taking outside to dump in the compost pile.  Never open contaminated tubs. jars or petri plates inside your house.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


Edited by Doc9151 (04/29/20 12:07 PM)


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Offlinespyrelx
Stranger

Registered: 05/09/10
Posts: 51
Last seen: 1 year, 2 months
Re: Quick green mold question [Re: Doc9151]
    #26637243 - 04/29/20 05:02 PM (3 years, 8 months ago)

Thanks to you both, I really appreciate the support this site gives.  To answer the question above I did sterilize between jars with a flame, so I assume it was in the substrate or the spore solution/hypodermic itself.  However, all the jars/substrate was sterilized the same way, and the jars innculated from the same hypodermic all look good, so I'm wondering how I got mold in this ONE jar and not the rest.  But I suppose mold is spores are tricky, and basically everywhere.  So maybe they just got a better hold on this jar than the others.

In any event, thanks again for the quick responses.  I really appreciate it.


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