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Offlinedonttouchmyhair
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Registered: 04/29/20
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Trich in my bags twice
    #26636320 - 04/29/20 09:04 AM (3 years, 9 months ago)

Hello everyone,

Newbie here. I was trying to inoculate two grain spawn bags but both bags got trich after showing some mycelium growth. Is there any reason this would happen to both? I sterilized the needle properly as a sidenote. I'm keeping them on a shelf in a dimly lit area if that's relevant. Any help is appreciated



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OfflineOm Namo Shivaya


Registered: 05/03/15
Posts: 749
Last seen: 16 days, 2 hours
Re: Trich in my bags twice [Re: donttouchmyhair]
    #26636336 - 04/29/20 09:11 AM (3 years, 9 months ago)

Did you cook these bags yourself or order them online? If you cooked them any details on the methods you followed? And how did you sterilize the needle? Did you inoculate in a SAB or in open air?


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InvisiblebodhisattaMDiscordReddit
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Re: Trich in my bags twice [Re: donttouchmyhair]
    #26636346 - 04/29/20 09:15 AM (3 years, 9 months ago)

Quote:

donttouchmyhair said:
Hello everyone,

Newbie here. I was trying to inoculate two grain spawn bags but both bags got trich after showing some mycelium growth. Is there any reason this would happen to both? I sterilized the needle properly as a sidenote. I'm keeping them on a shelf in a dimly lit area if that's relevant. Any help is appreciated





Because you inoculated them with a needle. Which implies a spore syringe. And spores are not axenic.

Spores to grain. Expect a lot of fail


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OfflinePSMM
PsychedelicsSavedMyMarriage
Trans-female


Registered: 03/27/20
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Re: Trich in my bags twice [Re: Om Namo Shivaya]
    #26636349 - 04/29/20 09:17 AM (3 years, 9 months ago)

I have had zero luck with pre-sterilized bags or jars.  Not a single one that I've bought worked for me.  Also, most of the spore syringes I have acquired have seemed to be contaminated as well.  If you purchased this bag pre-sterilized, there's a good chance it wasn't actually sterile.  If you PC'd it yourself, then something must have gotten in there afterwards.  I was getting extremely discouraged because I figured my best bet was to buy from someone who knew what they were doing, and when that failed I thought maybe this was just too difficult to do.  But then I got my own pressure cooker, and that's when things finally started happeneing!


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
Re: Trich in my bags twice [Re: PSMM]
    #26636370 - 04/29/20 09:26 AM (3 years, 9 months ago)

First off how did you clean your needle, second where did you acquire your bags, how did you prep your bags, and could perhaps be detailed on how you prepped them.
Thanks! :smbfacepalm:


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Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


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InvisiblebodhisattaMDiscordReddit
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Re: Trich in my bags twice [Re: mushhead]
    #26636516 - 04/29/20 10:42 AM (3 years, 9 months ago)

He said he sterilized the needle. Doesn't matter it's what's in the syringe.
Bags from anywhere don't just trich so who cares. Trich causes trich. They're probably premade though.


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Invisiblemushhead
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I'm a teapot


Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
Re: Trich in my bags twice [Re: donttouchmyhair]
    #26636592 - 04/29/20 11:15 AM (3 years, 9 months ago)

Quote:

donttouchmyhair said:
Hello everyone,

Newbie here. I was trying to inoculate two grain spawn bags but both bags got trich after showing some mycelium growth. Is there any reason this would happen to both? I sterilized the needle properly as a sidenote. I'm keeping them on a shelf in a dimly lit area if that's relevant. Any help is appreciated




WHelp - Here's what i'd do OP.
Get a pressure cooker - Presto 23qt works really well.
Get yourself widemouth jars and make your own spawn using some grain.
rye, wheat, and oats are the best from what I gather.
Purchase your MS from a reputable vendor, you can find a bunch on the vendor list.
Build yourself an SAB or Flow Hood - You have no idea how awesome a flow hood is until you try one.
Be clean yourself, I wash up my arms with hot soapy water before doing any kind of mushroom work, even if its just picking a flush.
Find a good tek and follow it - I'm sure bod has a few you can flush out using the search. Good luck OP!


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


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