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Roundabout
On again off again

Registered: 04/02/20
Posts: 80
Last seen: 1 year, 9 months
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Various species transfer bonanza, which looks best?
#26635677 - 04/28/20 11:58 PM (3 years, 9 months ago) |
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I got back into this after doing gourmets a decade ago and I'm on my first set of transfers. Curious what you look for in transfers. The rhizomatic grow seems faster and thus most appealing but I don't seem to have that for each species/variety.
Almost all of these transfers are 9 days old aside from the ps cyanescens which was slower and didn't get transferred until a few days ago. I also put some of the initial plate onto grain as I'm in a bit of a hurry to get wood lovers ready, and also some cubes fruited so I take another trip. I have lots more grain and bags available so will likely do another set of grain transfers and maybe some LC for the wood lovers.
Golden teacher, the rhizomatic growth on the plate on the right looks the best to me.

Burmese PE - The plate on the left has a bit of rhizo growth I could try to get, otherwise the center seems strongest and most consistent. The right is very fluffy

This is my second try with this B+ syringe, this time using a loop, looks like the syringe has a lot of bacteria though, 3 days later and it's already got those slimy dots. My first set of plates with this syringe did the same thing. I'll try the other B+ syringe I received next work session.
Tampanensis Galindoi, the one on the right seems the most consistent and fastest growing. so was planning to go for it. Or perhaps leading left edge of the right plate.
I did my first transfer of the ps cyanescens over the weekend and the right plate is looking like a winner already. Closeup of it

The azurescens is pretty lumpy, probably the left plate?

My initial plates were really clean other than the so I also transferred each to 2-3 grain jars. They seem to be growing quite slow. This is a transfer size piece of agar after 9 days on grain. Is this normal? All of them are roughly this size. They are growing just seems slow. This is on wheat which I had leftover from a decade ago, it cooked up fine though.
Another example from the Golden Teacher

Appreciate the feedback. All my prior experience was long ago, and was with gourmets where I just purchased liquid culture. I did one shiitake spore experiment but lost interest back then.
This early phase is both exciting, but also a little boring with a little bit of grain, agar, or LC prep, and then maybe 30mins of work in the hood each week and mostly just waiting for growth. I fear my plans for wood lovers is not going to be in time to catch a fall flush but we'll see. In addition to the above I'm also trying to get clean plates of B+, Rustywhyte, and Pan Cyanescens. Covered all my bases in one big order lol.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 16 hours
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Re: Various species transfer bonanza, which looks best? [Re: Roundabout]
#26635934 - 04/29/20 03:41 AM (3 years, 9 months ago) |
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I would do at least one transfer before putting to grain. Certainly never from a germination plate, the myc might have grown over bacteria or mold. Be patient, transfers only take a couple days. Better than losing a grow!
I make my first transfers as soon as I see clean, healthy growth. Usually when they are dime size or so.
Just look for the cleanest, healthiest looking mycelium that is the furthest away from any bacteria or mold colonies.
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 Amateur Mycologists United AMU Q&A
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