|
Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,274
Last seen: 3 hours, 34 minutes
|
New Paper: Synthesis and Biological Evaluation of Tryptamines Found in Hallucinogenic Mushrooms 1
#26635481 - 04/28/20 09:45 PM (3 years, 8 months ago) |
|
|
Today I came across this new paper on the alkaloids in psilocybin mushrooms, published in February 2020.
They suggest that baeocystin might not be psychoactive according to animal and cell culture assays, though Paul Stamets says he tried 10 mg and it did something. "Baeocystin has been detected alongside psilocybin in some varieties of mushrooms in concentrations substantial enough to be potentially physiologically relevant, up to about one-third the relative concentration of psilocybin."
In this study, alkaloids were purified using flash column chromatography and tested for activity using 5-HT2A receptor functional assays.
https://pubs.acs.org/doi/10.1021/acs.jnatprod.9b01061 ;
Full text: https://sci-hub.tw/https://pubs.acs.org/doi/pdf/10.1021/acs.jnatprod.9b01061
|
Blazer420
ŦøжїϿ ÐȐȜȧƜƐȓ


Registered: 06/13/09
Posts: 4,825
|
Re: New Paper: Synthesis and Biological Evaluation of Tryptamines Found in Hallucinogenic Mushrooms [Re: Alan Rockefeller]
#26635491 - 04/28/20 09:49 PM (3 years, 8 months ago) |
|
|
Very interesting read. Thanks for the links
-------------------- ~ I used to get high on life, until I realized life was cut with morons ~ * You need 2 wake up and smell the music! * -We are all computer data in a materialistic world- |Sometimes you have to lose yourself, to find anything|
 
|
Zombi3
Bella Ciao!!



Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
|
Re: New Paper: Synthesis and Biological Evaluation of Tryptamines Found in Hallucinogenic Mushrooms [Re: Alan Rockefeller]
#26635514 - 04/28/20 09:57 PM (3 years, 8 months ago) |
|
|
Interesting I was actually just talking about this with someone the other day, this is a perfect link to show them. Great read honestly. Thanks for sharing!
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
|
24sevenZed
~Z3D3Z~



Registered: 11/05/16
Posts: 90
Loc: CYBERSPACE
Last seen: 3 years, 7 months
|
Re: New Paper: Synthesis and Biological Evaluation of Tryptamines Found in Hallucinogenic Mushrooms [Re: Zombi3]
#26635675 - 04/28/20 11:57 PM (3 years, 8 months ago) |
|
|
Seems that there are two factors that make these compounds able to both cross metabolic barriers (in humans, at least) and provide action at 5-HT2 receptors: the position 4 functionalization on the indole ring and the second amination. And - they might both play a role in both parts, when you think about DMT.
Since, according to this paper, norpsilocin (the dephosphorylated analog of baeocystin) is technically _more_ active than psilocin, at least in assay:
“Taken collectively, the HTR data and 5-HT2A functional experiments would imply that baeocystin’s lack of CNS activity is possibly related to pharmacokinetics but not lack of activity at 5-HT2A receptors.”
Put this together with the recent paper about mushrooms also producing betacarbolines and you get more evidence for a very wide variation in psychoactive effects across species... in theory? I haven’t actually read that paper though, yet.
EDIT: meant second methylation
Edited by 24sevenZed (04/29/20 12:01 AM)
|
Mycoactive
Scientist


Registered: 11/20/19
Posts: 185
Last seen: 26 days, 21 hours
|
Re: New Paper: Synthesis and Biological Evaluation of Tryptamines Found in Hallucinogenic Mushrooms [Re: 24sevenZed]
#26637615 - 04/29/20 08:27 PM (3 years, 8 months ago) |
|
|
There are also 5-HT2A receptors in the peripheral nervous system, so it's possible (as the author's mention) that norpsilocin is activating those receptors. I'm curious if activating these receptors on peripheral neurons could induce some type of psychoactive effects by influencing neurotransmission in the CNS.
|
Moria841



Registered: 07/02/18
Posts: 4,929
Loc: NJ
Last seen: 2 hours, 20 minutes
|
Re: New Paper: Synthesis and Biological Evaluation of Tryptamines Found in Hallucinogenic Mushrooms [Re: Mycoactive]
#26637668 - 04/29/20 09:01 PM (3 years, 8 months ago) |
|
|
It's also very possible that baeocystin itself is not active, but instead is a prodrug for a different trypamine that is converted by some enzyme. This would explain why 5-HT2A assays didn't detect activity, but ingestion did
|
Psilosadhu



Registered: 12/19/19
Posts: 1,887
|
Re: New Paper: Synthesis and Biological Evaluation of Tryptamines Found in Hallucinogenic Mushrooms [Re: Moria841]
#26637725 - 04/29/20 09:38 PM (3 years, 8 months ago) |
|
|
Interesting
|
HSapiensAmericanus
Stranger
Registered: 01/15/20
Posts: 337
Last seen: 4 months, 13 days
|
Re: New Paper: Synthesis and Biological Evaluation of Tryptamines Found in Hallucinogenic Mushrooms [Re: Psilosadhu]
#26638001 - 04/30/20 12:46 AM (3 years, 8 months ago) |
|
|
Be interesting to know If the results change if they figured out the pharmacokinetics problem, but then again to figure that out it likely would end up a different tryptamine altogether. Maybe if they could piggy-back it on adrenaline or something similar and fast acting to excite the CNS and receptor uptake. Skydiving on MAOI’s and baeocystine might be a trip...
I only skimmed. Forgive me if I am missing something important.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: ToxicMan, inski, Alan Rockefeller, Duggstar, TimmiT, Anglerfish, Tmethyl, Lucis, Doc9151, Land Trout 258 topic views. 1 members, 21 guests and 12 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|